| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 172,755 | 0 | A | G | 55.6% | 9.2 / 20.5 | 18 | T388A (ACG→GCG) | clcA | H(+)/Cl(‑) exchange transporter ClcA |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base G (10/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGC‑GCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACC > NZ_CP009273/172676‑172841 | ggCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTAt < 1:56902/90‑1 (MQ=255) tGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTTCTATTTCCg > 1:304598/1‑90 (MQ=255) gtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATgggg < 1:394514/90‑1 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGGGGTTGCTTTTTCCGGGATgggggga > 2:394514/1‑88 (MQ=255) cTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTGCCGGGATtggggga > 1:434896/1‑88 (MQ=255) gggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTTTTGCCGGGATgggggggtt > 1:275536/1‑86 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTTTTATTTCCGGGATGGGGGGATTACTgg > 1:555168/1‑90 (MQ=255) ccGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTTTTGTCGGGATGGGGGGATTACTgg > 2:337548/1‑90 (MQ=255) cGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTggc < 2:425160/90‑1 (MQ=255) ttGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGC‑GCCGt < 1:432067/90‑1 (MQ=255) gAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGAGTTTTCTTTTGCCGGAATGGGGGGATTTCTTGCGGGACCTTTTTGCGC‑Gcccgtt > 2:432067/1‑86 (MQ=255) gTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTAtttc > 2:525555/1‑40 (MQ=255) gTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGc < 1:525555/42‑1 (MQ=255) gTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGC‑GCCGTTAACggg < 1:337548/90‑1 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTTCCGGGATGGGGGGATTACTTGCGGCATCTATTTGCGC‑GCCGGTTACggggg > 2:80394/1‑89 (MQ=255) ttCCGCAATATCACCTTGAGGCGGGGGCGGTTGCTATTGCCGGGATGGGGGGATTTCTGGCGGCATCTATTTGCGCGGCGGTTAAGgggg > 1:368761/1‑90 (MQ=255) ggggGCGTTTGCTATTGCCGGAATGGGGGGATTACTGGCGGCATCTATTCGCGC‑GCCGTTAACGGGGGTCATTCTGGGTCTGGGGATGAc > 2:252107/1‑90 (MQ=255) gggACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGC‑GCCGTTAACGGGGATCATTCTGGTTCTGGAGATGAcc < 1:176514/90‑1 (MQ=255) | GGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGCATTACTGGCGGCATCTATTCGCGC‑GCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACC > NZ_CP009273/172676‑172841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |