| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,791 | G→A | intergenic (+160/‑32) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,791 | 0 | G | A | 95.5% | 76.1 / ‑3.5 | 22 | intergenic (+160/‑32) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (13/8); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAA > NZ_CP009273/49707‑49873 | agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg > 1:468522/1‑90 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGAc > 1:245215/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAt > 2:406399/1‑90 (MQ=255) cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAtttttttt > 2:287075/1‑90 (MQ=255) cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAtttttttt > 2:235332/1‑90 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa < 2:463267/90‑1 (MQ=255) aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaaa < 1:287075/90‑1 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:482806/90‑1 (MQ=255) tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg < 1:272601/90‑1 (MQ=255) gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa > 1:497861/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:418915/1‑84 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:418915/84‑1 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 2:228616/1‑90 (MQ=255) gacgacGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGAt < 1:406399/90‑1 (MQ=255) gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa > 1:303417/1‑90 (MQ=255) ttACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATc < 1:411823/90‑1 (MQ=255) tttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATg > 2:265230/1‑90 (MQ=255) tttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATg > 2:265231/1‑90 (MQ=255) ttACGTATAATGGCGACAATTTTTTTTATCGGGaa < 2:531467/35‑1 (MQ=38) ttACGTATAATGGCGACAATTTTTTTTATCGGGaa > 1:531467/1‑35 (MQ=38) ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg > 2:416598/1‑90 (MQ=255) aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa < 1:527868/90‑1 (MQ=255) | AGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAA > NZ_CP009273/49707‑49873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |