| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,905 | T→G | L28R (CTC→CGC) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,905 | 0 | T | G | 96.0% | 79.1 / ‑3.2 | 25 | L28R (CTC→CGC) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (10/14); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01 | |||||||||||
CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49828‑49991 | cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaa < 1:342130/90‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGGTTaaa > 2:273265/1‑90 (MQ=255) tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCTATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACg > 2:533405/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCaa < 1:23057/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAac < 2:619997/90‑1 (MQ=255) gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACAcc > 1:418650/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt < 2:229092/90‑1 (MQ=255) cggcgTTAGCGGTAGACCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt < 2:468522/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt > 2:632238/1‑90 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt > 2:561883/1‑90 (MQ=255) gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTta < 1:588879/90‑1 (MQ=255) aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaaataa < 1:416598/90‑1 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCTATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt < 1:533405/90‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa > 2:38664/1‑60 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa < 1:38664/60‑1 (MQ=255) aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc < 1:561883/90‑1 (MQ=255) cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg > 2:47684/1‑90 (MQ=255) cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa < 2:418650/90‑1 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:228616/90‑1 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt < 2:382086/53‑1 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCt > 1:382086/1‑53 (MQ=255) ccATGCCGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaat > 2:524579/1‑90 (MQ=255) cctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt < 2:187750/47‑1 (MQ=255) cctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt > 1:187750/1‑47 (MQ=255) aTCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc < 2:591818/90‑1 (MQ=255) | CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49828‑49991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |