| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,372,536 | T→A | I26N (ATC→AAC) | epmA → | elongation factor P‑‑(R)‑beta‑lysine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,372,536 | 0 | T | A | 81.8% | 51.8 / 4.2 | 22 | I26N (ATC→AAC) | epmA | elongation factor P‑‑(R)‑beta‑lysine ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base A (9/9); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGAGCGAAACGGCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGATCCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGTATGAGCCAGGCGACGGTAACCGATATTCATTTGGTCC > NZ_CP009273/4372461‑4372622 | tGAGCGAAACGGCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGATCCGTCGTTTCTTTg > 1:300264/1‑90 (MQ=255)tGAGCGAAACGGCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAAAAACGCGGTTTCtttt > 2:69459/1‑89 (MQ=255) ggCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTgg < 1:624548/90‑1 (MQ=255) tGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTg < 2:177261/90‑1 (MQ=255) aTCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGcc < 2:399521/90‑1 (MQ=255) tacATTCCTAACATACAAAAACGAGCGGCGATTATGGCGCAGAACCGTTGTTTCTTTGCAAATCGTGGAGTGCTGGAGGTGGAGACGCCt < 2:318914/88‑1 (MQ=255) tCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGATCCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCt < 2:95373/90‑1 (MQ=255) tCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCt < 1:213266/90‑1 (MQ=255) aTTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGt > 1:107288/1‑90 (MQ=255) ttCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTg > 1:317735/1‑63 (MQ=255) ttCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTg < 2:317735/63‑1 (MQ=255) tattaAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTgg > 1:524189/1‑55 (MQ=255) tattaAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTgg < 2:524189/55‑1 (MQ=255) attaAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGGGGAGACGCCTTGTATGAGCCAGGc > 1:594538/1‑90 (MQ=255) taAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTTCCGATCGTGGAGGGCTGGGGGGGGGGACGCCTTGTATGAGCCAGGCGa > 2:181159/1‑90 (MQ=255) taAAACGCGCGGCGATTATGGCGGAGAACCGTCGTTTCTTTTCCGATCGTGGAGGGCTGGAGGGGGGGACGCCTTGTATGAGCCAGGCGa > 2:178064/1‑90 (MQ=255) cgcgcgGCGATTATGGCGGAGATCCGTCGTTTCTTTGCCGATCGTgggg > 2:327426/1‑47 (MQ=255) cgcgcgGCGATTATGGCGGAGATCCGTCGTTTCTTTGCCGATCGTGGAg < 1:327426/49‑1 (MQ=255) cgcgcgGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGt < 1:619403/59‑1 (MQ=255) cgcgcgGCGATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGt > 2:619403/1‑59 (MQ=255) ggcgATTATGGCGGAGAACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGTATGAGCCAGGCGACGGTAACCGa < 1:181159/90‑1 (MQ=255) agaACCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGTATGAGCCAGGCGACGGTAACCGATATTCATTTGGTcc > 1:100049/1‑90 (MQ=255) | TGAGCGAAACGGCATCCTGGCAGCCGAGCGCATCCATTCCTAACTTATTAAAACGCGCGGCGATTATGGCGGAGATCCGTCGTTTCTTTGCCGATCGTGGAGTGCTGGAGGTGGAGACGCCTTGTATGAGCCAGGCGACGGTAACCGATATTCATTTGGTCC > NZ_CP009273/4372461‑4372622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |