| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,031 | 0 | T | G | 53.3% | 11.7 / 15.7 | 15 | V211G (GTT→GGT) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATG > NZ_CP009273/699961‑700110 | ggTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGCGACATTAAc > 2:550434/1‑90 (MQ=38) tCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTCCCCGGGGGCATTAACCg > 2:129224/1‑90 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCCCGGGGGCATTAACCGCTTCTATGCCggg > 2:103616/1‑89 (MQ=255) gAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACCGGGACATTTACCGCTTCTATGCCTGtct > 2:382148/1‑88 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 1:157879/90‑1 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 1:378554/90‑1 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 2:44091/90‑1 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGGGGCATTAACCGCCTCTATGGCGGTggcggcc > 2:309137/1‑89 (MQ=255) ccTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCgg < 2:391016/90‑1 (MQ=255) tCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGGGGCATTAAACCCTTCTATGCCGGGGGCGGGACCGCGGGGgtgt > 1:296161/1‑90 (MQ=255) cACCAACGCGGCGGGGACGGGTTTCCACGGGGACATTAACCGCTTCTATGCCGGGGGCGGCACCGCGGGGGTGTTCATGTCCGGcttctt > 2:201618/1‑90 (MQ=255) cAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTccc < 1:309137/90‑1 (MQ=255) aCGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGGTGTTCATGTCCGGCTTCTTCCCGa > 1:549058/1‑90 (MQ=255) gcggcgGGGACGGGTTTTCACGGGGACATTAACCGCTTCTATGCCGGGGGCGGGCCCGCGGGGGGGGTTATGTCCCGCTTCTTCCCGATc > 2:366759/1‑90 (MQ=255) gcggGGACGGGTTTTCACGGGGACATTAACCGCTTCTATGCCGGGGACGGCACCGCGGGGGTGTTCATGTCCGGCTTCTTCCCCATCatg > 2:216165/1‑90 (MQ=255) | GGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATG > NZ_CP009273/699961‑700110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |