| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,978,066 | 0 | T | G | 53.3% | 10.6 / 14.3 | 15 | T296P (ACC→CCC) | yqeF | acetyl‑CoA C‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAAC > NZ_CP009273/2977977‑2978154 | cGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGt < 2:225216/90‑1 (MQ=255) ccTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATaca > 1:362706/1‑90 (MQ=255) tgtgCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACAcc < 1:27431/90‑1 (MQ=255) aaaCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATAAACCCgcgccaa > 1:261805/1‑87 (MQ=255) aTTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCaa < 2:464650/90‑1 (MQ=255) ccTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCg < 1:433060/90‑1 (MQ=255) gACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCAATTCCCCTCAAAGCCGGGGCTACACCAAc > 1:372915/1‑90 (MQ=255) gACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCAAATCCCCTCCAAACCGGGTCTACACCAAc > 1:501915/1‑90 (MQ=255) aCTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCCCCAATCCCCTCCAAACCCGGTCTACACCAACCCCGGGaaaag > 2:546153/1‑88 (MQ=255) acGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCAATTCCCCTCAATTCCGGGTCTACACCCACCCCGGCAAAAACGCGGAAGCgggg > 1:303444/1‑89 (MQ=255) cTCCAGGCAACGGCGGGTCGCATACACCGGCGCCATTCTCCACAATCCCGGTCTTACACCCACCCCGGGAAAAGCGCGAAAGGGGGGCAg > 1:201408/1‑90 (MQ=255) ccAGGCAACGGCGGGGCGCATACACCCGCGCAAATCCCATCAAAGCCCGGTCTACACCAACCCCGGCAAAAGCGCGGAAGCGGGCCCGCa > 2:220125/1‑90 (MQ=255) cAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCAc < 1:403438/90‑1 (MQ=255) cggcggGGCGCATACACCCGCGCAATTCCCCTCAAAGCCGGGTCTACACCAACGCCGGCAAAAGCGCGAATGCGGGGCAGCACGGGGaaa > 1:392641/1‑90 (MQ=255) gcggGGCGCATACACCCGCGCCATTCCCCTCAATACCCGGTCTACACCCACGCCGGCAAATGCGCGAAAGCGGGGCCGCACGGGGaaaat > 2:204796/1‑88 (MQ=255) gTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAAc < 2:392641/90‑1 (MQ=255) | CGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAAC > NZ_CP009273/2977977‑2978154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |