| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,775,223 | 0 | T | G | 68.4% | 9.7 / 15.2 | 19 | T234P (ACC→CCC) | cysE | serine O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (13/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGCGCC > NZ_CP009273/3775140‑3775310 | gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATgcgg > 2:114731/1‑90 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATgcgg > 2:416467/1‑90 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATgcgg > 2:436758/1‑90 (MQ=255) ttGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATggggc > 2:421458/1‑90 (MQ=255) gCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCg < 1:240270/90‑1 (MQ=255) tGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCAcccgg > 1:513712/1‑87 (MQ=255) atatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGGGGCGGCACCGGGTtcagg > 1:385295/1‑89 (MQ=255) tatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCGGTTgcagca > 1:213790/1‑90 (MQ=255) tatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGCTGCGGCGGCCCCCGTTtcagga > 2:320059/1‑88 (MQ=255) aTTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGGGGCACCGGTTGCAGCACCACg > 1:42930/1‑90 (MQ=255) ttGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGGGGGGGGACCCGTTGCAGCACCACgg > 2:380279/1‑90 (MQ=255) aTGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGGGGCGGCCCCCGGTGCAGCACCACGGGAc > 2:445415/1‑90 (MQ=255) tGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAAcc < 1:380279/90‑1 (MQ=255) tACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTcgcgccgc < 2:537783/90‑1 (MQ=255) gACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACgg > 1:448028/1‑62 (MQ=255) gACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACgg < 2:448028/62‑1 (MQ=255) gACAATACGAGCCGGAACGCCAGCGGCGGGGGGATGCGGCGGCACCGGGTGCAGCACCACGGGAACCGCGCCAATCTTCCCGCCGCGccc > 2:489744/1‑90 (MQ=255) aCAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCa < 2:245859/90‑1 (MQ=255) aTACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACt > 1:87552/1‑90 (MQ=255) ggtATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTcgcgcc < 1:421458/90‑1 (MQ=255) | GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATATTGCCGAGGATTTTCGCGCC > NZ_CP009273/3775140‑3775310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |