Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,300,524	Δ1 bp	coding (504/1005 nt)	oppF →	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,300,522	0	G	.	100.0%	249.6 / NA	56	coding (502/1005 nt)	oppF	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (30/26);  total (30/26)

GAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTG  >  NZ_CP009273/1300435‑1300605                                                                                         |                                                                                    gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCtggtggc                                                                                   <  2:2317881/90‑2 (MQ=255) gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCtggtggc                                                                                   <  1:691843/90‑2 (MQ=255) gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCtggtggc                                                                                   <  2:2526277/90‑2 (MQ=255) gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCtggtggc                                                                                   <  2:706449/90‑2 (MQ=255) gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCtggtggc                                                                                   <  2:2663168/90‑2 (MQ=255)     ttCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTg                                                                              >  1:156825/1‑90 (MQ=255)        gcgAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCa                                                                           <  1:1592790/90‑1 (MQ=255)         cgAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAg                                                                          <  1:3008123/90‑1 (MQ=255)          gAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGc                                                                         <  2:1528818/90‑1 (MQ=255)          gAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGc                                                                         <  2:4393/90‑1 (MQ=255)              gcgTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt                                                                     >  1:1570813/1‑90 (MQ=255)              gcgTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt                                                                     >  1:2394346/1‑90 (MQ=255)                 tGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCgg                                                                  >  1:507130/1‑90 (MQ=255)                        gatgatGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTgcg                                                           >  2:183219/1‑90 (MQ=255)                         atgatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTgcgc                                                          >  2:677938/1‑90 (MQ=255)                           gatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  1:2132060/90‑1 (MQ=255)                           gatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  1:647172/90‑1 (MQ=255)                           gatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  1:1806604/90‑1 (MQ=255)                           gatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  1:1541977/90‑1 (MQ=255)                           gatgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  1:1313429/90‑1 (MQ=255)                            atgCTGAAAGTCGGGTTATTGCCTAACCTGGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  2:2328045/90‑1 (MQ=255)                            atgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTg                                                       <  1:2514680/90‑1 (MQ=255)                             tgCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGc                                                      >  2:1996749/1‑90 (MQ=255)                                 gAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt                                                  <  1:2537816/90‑1 (MQ=255)                                       cGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCtt                                            <  1:2011929/90‑1 (MQ=255)                                       cGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCtt                                            <  1:1215561/90‑1 (MQ=255)                                        gggTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTg                                           >  1:755333/1‑90 (MQ=255)                                        gggTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTg                                           >  2:760050/1‑90 (MQ=255)                                                 ccTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGc                                  <  1:2784466/90‑1 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGa                                     >  1:1062417/1‑85 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGa                                     <  2:1062417/85‑1 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTg                                >  1:609762/1‑90 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTg                                >  2:909629/1‑90 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTg                                >  2:1084606/1‑90 (MQ=255)                                                   tAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTg                                >  2:2021429/1‑90 (MQ=255)                                                    aaCCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGc                                  >  2:1923689/1‑87 (MQ=255)                                                    aaCCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGc                                  <  1:1923689/87‑1 (MQ=255)                                                     aCCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAt                              >  1:2238210/1‑90 (MQ=255)                                                          gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATc                          >  2:1563218/1‑90 (MQ=255)                                                           aTTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCt                         >  1:1392416/1‑90 (MQ=255)                                                             tAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCtgt                       <  1:2282648/90‑1 (MQ=255)                                                               aCCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt                                                                     >  2:2365282/1‑42 (MQ=39)                                                               aCCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt                                                                     <  1:2365282/42‑1 (MQ=38)                                                               aCCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGtga                     <  2:1570813/90‑1 (MQ=255)                                                               aCCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGtga                     <  1:491507/90‑1 (MQ=255)                                                                 cGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGtgatg                   >  1:1856494/1‑90 (MQ=255)                                                                    tATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGc                >  2:2760947/1‑90 (MQ=255)                                                                    tATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGc                >  1:719536/1‑90 (MQ=255)                                                                    tATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGc                >  2:175459/1‑90 (MQ=255)                                                                         gCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGgtg           >  2:1673112/1‑90 (MQ=255)                                                                          cATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGgtgt          >  2:2114966/1‑90 (MQ=255)                                                                           aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc         <  2:2907053/90‑1 (MQ=255)                                                                           aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc         <  2:1325663/90‑1 (MQ=255)                                                                           aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc         <  1:1283332/90‑1 (MQ=255)                                                                            tGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCg        <  1:860349/90‑1 (MQ=255)                                                                             gAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        >  1:2886960/1‑41 (MQ=39)                                                                             gAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                        <  2:2886960/41‑1 (MQ=39)                                                                             gAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCgg       >  2:370478/1‑90 (MQ=255)                                                                               gTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGgcg     >  1:156001/1‑90 (MQ=255)                                                                                 tctcTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCt   >  2:1829900/1‑90 (MQ=255)                                                                                  ctctGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTg  >  2:963347/1‑90 (MQ=255)                                                                                         |                                                                                    GAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCT‑GATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTG  >  NZ_CP009273/1300435‑1300605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑