| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,020 | 0 | A | C | 46.3% | 88.7 / 33.6 | 67 | *559E (TAA→GAA) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (32/4); new base C (0/31); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGA > NZ_CP009273/496931‑497105 | ggCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTa > 1:911151/1‑90 (MQ=255) aTTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc < 1:1636898/90‑1 (MQ=255) tttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcccc > 1:47830/1‑90 (MQ=255) ggCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTc < 1:1664726/90‑1 (MQ=255) cGATGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCaccc‑cc < 1:1125852/90‑1 (MQ=25) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCAc > 1:525491/1‑90 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCAc > 2:2860992/1‑90 (MQ=255) gCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCAc > 2:2769197/1‑90 (MQ=255) gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc < 1:2817389/72‑1 (MQ=255) gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc > 2:2817389/1‑72 (MQ=255) gACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTg > 1:1518830/1‑90 (MQ=255) aCGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTgg > 2:2767590/1‑90 (MQ=255) gCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCAACCACCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCg < 2:1443327/90‑1 (MQ=38) ggCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCGttt < 2:904309/90‑1 (MQ=255) ggCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGttt > 2:2595432/1‑90 (MQ=255) ggCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGttt > 1:83790/1‑90 (MQ=255) cgTCTTATCATGCCTACATATTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGCCCCCC‑CCTCCCCCGCCGGTGGCGTTTCcag < 2:905061/90‑1 (MQ=255) gTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCGTTTCcagc < 2:757663/90‑1 (MQ=255) gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCcagc > 2:780066/1‑90 (MQ=255) gTCTTATCATGCCTAAATTTTTTTCATTTTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCA‑CCTCACCCGCCGGTGGCGTTTCcagc < 2:1261114/90‑1 (MQ=255) gTCTTATCATGCCTAAATTTTTTTCATATTTTACACCCGGAACCCCCCGTTTCCCCCGCCACCC‑CCTCCCCCGCCGGTGGCGTTTCcagc < 2:1434840/90‑1 (MQ=255) tCTTACCATCCCTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCGTTCCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCcagca < 1:1440888/90‑1 (MQ=255) ttATCATCCCTACTTTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGt < 1:1195285/90‑1 (MQ=255) tCATGCCTACATTTTTTTAATTTTTTACATCCGGCAACCACCTTTTCCCCCGTCACCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:525491/90‑1 (MQ=255) tCATCCCTAAATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:1868870/90‑1 (MQ=255) tCACGCCTACATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTcc < 2:3020474/90‑1 (MQ=255) tcccTATATTTTTTTCATTTTTTACCTCCGACAACCCCCTTTTCCCCCGCCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGc < 1:1517407/88‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTca > 1:2547533/1‑51 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTca < 2:2547533/51‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:2786774/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 1:2251057/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:1150623/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:1156060/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 1:1870452/1‑90 (MQ=255) ccaaCATTTTTTTTAT‑TTTTCCCCCCCGCAACCCCCCTTTCCCCCCCCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:1285980/87‑1 (MQ=255) ccTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCTTTTCCCCCCCCCCCC‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:2118307/90‑1 (MQ=255) ccTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:2251057/90‑1 (MQ=255) ccTACATTTTTTTCATTTTTTACCCCCGCCACCCCCCGTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:110093/90‑1 (MQ=255) ccTACATTTTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:346170/90‑1 (MQ=255) ccTACATTTTTTTATTTTTTTACACCCGCCACCCCCCTTTCCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:972522/90‑1 (MQ=255) ccTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt > 2:1285980/1‑90 (MQ=255) tACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg > 2:1773673/1‑90 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt > 2:1073531/1‑90 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt > 2:2253265/1‑90 (MQ=255) atttttttCATTTTTTACACCCGGCACCCCCCTTTTCCCCCCCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc < 1:780066/87‑1 (MQ=255) tttttccatcttttccatcCGCCCCCCCCCTTTTCCCCCCTCCCCA‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACgg < 2:1659209/89‑1 (MQ=255) tttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg > 2:1736688/1‑90 (MQ=255) tttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg > 2:367193/1‑90 (MQ=255) tttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg > 1:2339073/1‑90 (MQ=255) ttttCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc < 1:1073531/90‑1 (MQ=255) ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc > 2:351763/1‑90 (MQ=255) ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc > 2:2944765/1‑90 (MQ=255) cATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt > 2:421337/1‑90 (MQ=255) cATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt > 1:2607428/1‑90 (MQ=255) tccATCCGGCACCCACCTTTTCCCCCGCCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 2:492241/88‑1 (MQ=255) tACATCCGGCACCCCCCGTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 1:2247774/90‑1 (MQ=255) aCATCCGGCAACCCCCGTTTCCCCCGTCCCCC‑CCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc < 2:252063/90‑1 (MQ=255) cgccGCCAACCGCTGTTTCCCCCTCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc < 1:2412653/88‑1 (MQ=255) cccGGCACCCCCCTTTTCCCCCGCCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 2:2882586/89‑1 (MQ=255) cccGGCACCCCCCGTTTCCCCCGTCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc < 2:927768/89‑1 (MQ=255) ccGGCACCCCCCTTTTCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc < 1:367193/90‑1 (MQ=255) ccGGCACCCCCCGTTTCCCCCGCCCCCC‑CCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGccc < 2:424924/90‑1 (MQ=255) cGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCa > 1:1397676/1‑90 (MQ=255) ggCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 2:1145692/1‑90 (MQ=255) ggCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt > 1:1760150/1‑90 (MQ=255) accGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc > 1:2883696/1‑90 (MQ=255) ccttttCCCCCGCCCCCC‑CCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCt < 2:1303868/87‑1 (MQ=255) gTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGa > 2:2344802/1‑90 (MQ=255) | GGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCA‑CCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGA > NZ_CP009273/496931‑497105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |