| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,032 | 0 | T | G | 46.7% | 36.6 / 57.9 | 60 | V288G (GTG→GGG) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base T (1/31); new base G (28/0); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.80e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAG > NZ_CP009273/594943‑595121 | tCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt < 1:2556948/90‑1 (MQ=255)tCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt < 1:180200/90‑1 (MQ=255) tGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGgggggggggg > 1:494831/1‑90 (MQ=255) gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggg > 2:2135506/1‑89 (MQ=255) gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggg > 2:16805/1‑89 (MQ=255) gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggggg > 2:1469158/1‑87 (MQ=255) gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGa < 1:872233/90‑1 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCTGCGGT‑CCgg > 2:1574840/1‑90 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGTCTGCGAT‑cccg > 1:732731/1‑88 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAT‑CCgg > 2:742737/1‑90 (MQ=255) ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTACCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGT‑cccg > 2:147625/1‑88 (MQ=255) gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAG‑CCGGCgaaaa > 1:1535955/1‑90 (MQ=255) aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAAc < 2:1290976/90‑1 (MQ=255) aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAAc < 2:1382707/90‑1 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGATGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAT‑CCGGCAAAAACGcc > 2:829053/1‑90 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCcaaaaaacc > 1:2843556/1‑86 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCaaaaaaacc > 2:2365925/1‑86 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCCGCcaaaaaacc > 1:320560/1‑86 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt < 2:1279165/62‑1 (MQ=255) gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGAT‑CCCGCAAAAAAGccc > 1:548901/1‑90 (MQ=255) gCCCGGAGAGGCGCCGGGGAATTCCCGACCAAACCGGC‑AAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCg < 2:769818/90‑1 (MQ=255) ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCgaga < 2:743809/90‑1 (MQ=255) ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGAT‑CCGGCAAAAAAGCCCgaga > 1:996875/1‑90 (MQ=255) gagaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCTGCGGT‑CCGGCAAAAAAGCCCGGGaa > 1:2584068/1‑90 (MQ=255) gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTCCGGT‑CCCGCAAAAAAGCCCGCGAAgg > 1:1960067/1‑89 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCCAAAAAGCCCGAGAAGGGAt > 1:492239/1‑90 (MQ=255) gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCTGCGGA‑CCCGCGAAAAAGCCCGAGAAggggt > 1:388171/1‑86 (MQ=255) cgcgGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATc < 1:2125155/90‑1 (MQ=255) ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgc < 1:2263712/90‑1 (MQ=255) ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGGT‑CCGGCAAAAACGCCCCAGAAGGGATcgccg > 2:2943864/1‑90 (MQ=255) ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGT‑CCCGCGAAAAAGCCCGAGAAGGGGTcgccg > 1:2177439/1‑90 (MQ=255) ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGT‑CCGGCAAAAAAGCCCGAGAAGGGGTcgccg > 1:2928349/1‑90 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 1:1704054/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 1:1574840/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 2:2643332/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 1:16805/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 2:705090/90‑1 (MQ=255) gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATcgccgc < 1:829053/90‑1 (MQ=255) aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCGAAAAAACCCCCGAAGGGGGCgccgcccgg > 1:2643332/1‑87 (MQ=255) aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTg > 2:149215/1‑90 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGT‑CCGGCAAAAAAGCCCGGGAAGGGAGCGCCGCCGTGa > 2:1796133/1‑90 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCCGCCAAAAACCCCCG‑GAAGGGATCgccgcccgga > 2:1704054/1‑86 (MQ=255) tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGaa < 2:1621844/90‑1 (MQ=255) gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGT‑CCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAg > 2:1342568/1‑90 (MQ=255) gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGAc < 1:2041737/90‑1 (MQ=255) gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGAT‑CCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGAc > 2:46709/1‑90 (MQ=255) cTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa < 2:492239/90‑1 (MQ=255) gcgaaggcgaagTGGCGGGCGGGGGGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa > 2:1113279/1‑90 (MQ=255) cgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAc < 2:641903/90‑1 (MQ=255) aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc < 2:2630518/90‑1 (MQ=255) aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc < 2:2843556/90‑1 (MQ=255) ggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTg < 1:621511/90‑1 (MQ=255) ggcgaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTg < 1:3002323/90‑1 (MQ=255) ggcgaagTGGCGGGCGGGGGGGGGGTCCTGCGAA‑CCGGCAAAAAAGCCCGAGAAGGGGTCGCCGCCGGGGAGGACAAACAGGAAAAGCTg > 1:2167176/1‑90 (MQ=255) gaagTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaaa < 2:294053/90‑1 (MQ=255) agTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAag < 2:940108/90‑1 (MQ=255) ggcgggcgGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag < 2:392965/90‑1 (MQ=255) ggcgggcgGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag < 2:1879665/90‑1 (MQ=255) ggcgggcgGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag < 1:1979003/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:2504691/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:2727975/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 2:1920671/90‑1 (MQ=255) cgggcgggGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTc < 1:588393/90‑1 (MQ=255) cgggcgggGGGGGGATCCTGCGAT‑CCGGCCAAAAAGCCCGAGGAGGGATCGCCGCCGTGGAGGACAAAATGGGAAAGCTGAAAAAAAGTc > 1:1869412/1‑90 (MQ=255) gcgggGGGGGGGTCCTGCGAT‑CCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGGCAAAAAGGAAAAGCTGAAAAAAAGTCTGc > 2:2572080/1‑90 (MQ=255) ggggTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCg < 1:2406503/90‑1 (MQ=255) gggTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCgg > 1:1648714/1‑90 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:732731/90‑1 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 1:411911/90‑1 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:1501394/90‑1 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:1960067/90‑1 (MQ=255) ggtggtGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:22736/90‑1 (MQ=255) tggtgATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg < 2:134504/90‑1 (MQ=255) tggtgATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg < 2:500399/90‑1 (MQ=255) | TCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAG > NZ_CP009273/594943‑595121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |