| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,661 | 0 | A | C | 50.0% | 112.6 / 14.9 | 76 | G142G (GGT→GGG) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base A (37/1); new base C (0/38); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAT > NZ_CP009273/3571579‑3571748 | ggCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCa > 2:793611/1‑90 (MQ=255) cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACt > 2:145337/1‑90 (MQ=255) cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACt > 2:629515/1‑90 (MQ=255) cGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACt > 2:2527139/1‑90 (MQ=255) gTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACtt > 1:124238/1‑90 (MQ=255) gTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACtt > 2:617543/1‑90 (MQ=255) gTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACtt > 2:2037210/1‑90 (MQ=255) tgctgcTTAAAATTTATAAAAC‑CCGCCGGCAATACCAAGGCATGGCGTTTTGCTGTCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTc < 1:537682/90‑1 (MQ=255) ctgctAAAAATTTAAAAAAC‑CCGACGGCGATATAAAGGCTTGGCGCCTTGCTGCCCATCATTCCCCCCAGGGA‑AATCCCCGCCACTTCaa < 2:211776/90‑1 (MQ=255) gctTCAAATTTAAAAAAC‑CGGGCGGAAAAATCAAGGAAGGGCCCTTTGTTGTCCATCATTCCCCCCAGGGA‑AATCCCCGCCACTTCAatc < 1:2505382/90‑1 (MQ=255) gctTAAAATTTATAAAAC‑CCGGGGGCGATATAAAGGCAGGGCGATTTGCTGCCCATCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAatc < 2:348275/90‑1 (MQ=255) gctTAAAATTTATAAAAC‑CCGACGGCGATACAAAGGCAGGGCGACTTGCTGCCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAatc < 2:1715200/90‑1 (MQ=255) gctaaaaaTTAACAAAACCCGCGGGCAATACAAAGGCAGGGCGTTTTGCTTTCCATCATTCCCCCCAGGGA‑AATCCCCGCCACTTCAatc < 2:2573614/86‑1 (MQ=255) ttCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAatcat > 2:33854/1‑90 (MQ=255) ttCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAatcat > 1:787471/1‑90 (MQ=255) ttCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAatcat > 1:1510955/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 2:2842343/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 2:1136756/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 1:2183480/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 1:2505461/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 1:422108/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 2:501031/1‑90 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg > 1:660257/1‑90 (MQ=255) aaaaaaaa‑CCGACGGCAAAAAAAAGGCATGGCGCTTTGCTGCCCATCA‑TTCCCCCCCGGGAAATCCCCGCCACTTCAATCATCTTTAAgg < 2:2008524/86‑1 (MQ=255) aacccgccgGCGATAACAGGGCAGGGCGTTTTGCTCCCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 2:2253861/87‑1 (MQ=255) aacccgccgGCAATACAAAGCCAGGGCGCCTTCCTGCCCATCAGCCCCCCCAGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:1922804/87‑1 (MQ=255) aacccGGCGGCGATATCAGGGCGGGGCGTTTTGCTCTCCATCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 1:2819798/87‑1 (MQ=255) aacccGACGGCGATATAAAGGCAGGGCGACTTGCTGCCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 2:887578/87‑1 (MQ=255) aacccGACGGCAATACAAAGGCAGGGCGACTTGCTGTCCATCATTCCCCCCAGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTgcgc < 2:1020160/87‑1 (MQ=255) acccGACGGCGATATCAAGGCAGGGCGCCTTGCTGTCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGcgcg < 2:1108571/88‑1 (MQ=255) cccgccgGCAATAAAAAGGCATGGCGTCTTGCTGCCCACCAGTCCCCCCACGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 2:294431/89‑1 (MQ=255) cccgccgGCAATAAAAAGGCAGGGCGTCTTGCTCCCCATCATTCCCCCCGGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:586574/89‑1 (MQ=255) cccGGCGGCAATACCAAGGCATGGCGTTTTGCTGCCCACCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:458381/89‑1 (MQ=255) cccGGCGGCAAAATCAGGGCAGGGCGCTTTGCTGTCCATCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:408052/89‑1 (MQ=255) ccGACGGCGATATCAAGGCGTGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCgcggc > 1:2432431/1‑90 (MQ=255) cggcGGCGATATAAAGGCAGGGCGTCTTGCTGCCCATCAGTCCCACCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCgcggcg < 2:2432431/87‑1 (MQ=255) aCGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtg > 2:1026409/1‑90 (MQ=255) cGGCAATATCAGGGCATGGCGTTTTGTTCTCCATCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtgt < 1:721481/90‑1 (MQ=255) aatatCAGGGCGTGGCGCCTTGCTTTCCATCAGTCCCCCCGGGGA‑AACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGt < 1:2899258/89‑1 (MQ=255) atatCAGGGCAGGGCGCTTTGCTGTCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTa < 1:1157766/90‑1 (MQ=255) atatAAAGGCATGGCGTCTTGCTGCCCATCAGTCCCCCCGCGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTa < 2:1049171/90‑1 (MQ=255) acAAGGCATGGCGCCTTGCTGCCCATCATTCCCCCCACGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGt < 2:124238/89‑1 (MQ=255) agggCAGGGCGTCTTGCTGCCCATCATTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 2:2578839/88‑1 (MQ=255) agggCAGGGCGCCTTGCTGTCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 2:1912915/88‑1 (MQ=255) aaGGCGGGGCGCTTTGCTGCCCACCGGTCCCCCCGGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 2:1966715/90‑1 (MQ=255) aaGGCGGGGCGATTTGTTGTCCCTCATTCCCACCACGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 2:1717702/90‑1 (MQ=255) aaGCCAGGGCGCCTTTCTGTCCATCACTCCCCCCGGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 1:1811474/90‑1 (MQ=255) cATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg > 2:55120/1‑90 (MQ=255) cATGGCGACTTGCTGCCCATCATTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg < 1:119326/90‑1 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:2034618/1‑90 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:2027899/1‑90 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 1:1947241/1‑90 (MQ=255) tGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg > 2:2732643/1‑90 (MQ=255) cTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAg > 2:2263485/1‑90 (MQ=255) ttGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAgg > 2:2096007/1‑90 (MQ=255) cctttCCACCAGCCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc < 1:2203362/86‑1 (MQ=255) tCCATCATTCCCCCCGGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCg < 1:1136756/90‑1 (MQ=255) cccATCA‑TTCCCCCCCGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCg < 2:112993/89‑1 (MQ=255) catcatTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:881007/90‑1 (MQ=255) catcatTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:489434/90‑1 (MQ=255) catcatTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:33854/90‑1 (MQ=255) cATCAGCCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:250038/90‑1 (MQ=255) tCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTGCGTTCGGTGAGGATCAGGCCGTCGatat > 1:1903960/1‑90 (MQ=255) gTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc > 1:1645790/1‑90 (MQ=255) tCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg > 1:2429395/1‑90 (MQ=255) tCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg > 2:1612819/1‑90 (MQ=255) tCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg > 2:2359251/1‑90 (MQ=255) cccccagGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg < 2:943146/87‑1 (MQ=255) acGGG‑AATACGCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGTTACGTTCGTTGAGGATCAGGCGATCGATATTCCAGGagaga > 2:2221688/1‑89 (MQ=255) ggg‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgtgt > 2:739634/1‑44 (MQ=255) ggg‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 2:2415301/1‑90 (MQ=255) ggg‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 1:40720/1‑90 (MQ=255) g‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTGCGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATgg < 2:1903960/90‑1 (MQ=255) aTACCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 2:778393/35‑1 (MQ=255) aTACCCGCCACTTCAATCATCTTTAAGGTGCgcgg > 1:778393/1‑35 (MQ=255) aTACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAt > 1:1630069/1‑90 (MQ=255) aCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg > 1:2060424/1‑52 (MQ=255) aCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg < 2:2060424/52‑1 (MQ=255) | GGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAT > NZ_CP009273/3571579‑3571748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |