| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,673,542 | A→G | K287E (AAG→GAG) | ydgH → | DUF1471 family protein YdgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,542 | 0 | A | G | 98.7% | 241.8 / ‑5.8 | 78 | K287E (AAG→GAG) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/52); total (25/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
ATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATA > NZ_CP009273/1673455‑1673627 | atgatgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCggag < 2:316638/90‑1 (MQ=255) gatgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCggagaa < 2:2981346/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 2:882429/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:1483545/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:2986481/90‑1 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg < 1:379690/90‑1 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg > 2:1042178/1‑90 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg < 1:2344894/90‑1 (MQ=255) tCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTg > 1:2464787/1‑90 (MQ=255) cccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGc < 2:1817196/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 1:2453640/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:1005177/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:1609313/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa > 2:1905886/1‑90 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:2256885/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 2:489980/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:184754/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:2321365/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:1319752/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:2745164/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:36580/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:598835/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa > 2:958270/1‑90 (MQ=255) tCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGt < 1:1042178/90‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt < 2:1169554/90‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt < 2:2571693/90‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt < 1:378154/90‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt > 1:138926/1‑90 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt > 1:2994699/1‑90 (MQ=255) gCATCAAATTCTCTGGCAGCTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAcc < 1:1663772/90‑1 (MQ=255) gCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAcc > 1:697689/1‑90 (MQ=255) gCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAcc > 1:2616184/1‑90 (MQ=255) gCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTAcc > 1:2802019/1‑90 (MQ=255) ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:578405/72‑1 (MQ=255) ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa > 2:578405/1‑72 (MQ=255) ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCg > 2:1809690/1‑90 (MQ=255) tctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGc > 1:596481/1‑90 (MQ=255) tctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGc > 2:361774/1‑90 (MQ=255) cTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCa < 2:1500341/90‑1 (MQ=255) cGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 1:492271/58‑1 (MQ=255) cGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt > 2:492271/1‑58 (MQ=255) aaCTTGACCAAGGTCCCCTATAAGGTTGCAAACCGCGCCGGGGAAAAAGGTGCTAAGTTTTACCACACCCCCCGCCAGTGGCAGGAACGt < 2:2450726/90‑1 (MQ=255) aaCATGCCCGAAGTCTCCTATCGGGTTGCAAACCGGGCGGGGGAGAAAGGTGAAAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGt < 1:1640051/90‑1 (MQ=255) aaCATGCCCGAAGTCCCCTATCAGGTTGCAAAACGTGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGt < 2:2221306/90‑1 (MQ=255) aaCATGACCGAGGTCTCCTATCGGGTTGCGAAACGGGCCGGGGAAAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGt < 1:916799/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCGGGTTGCGAAACGGGCCGGGGAAAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGt < 1:766143/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGGGGAAAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGt < 1:984346/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCAAAACGTGCCGGGGAAAAAGGTGCAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGt < 1:284306/90‑1 (MQ=255) aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt > 1:2587301/1‑51 (MQ=255) aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 2:2587301/51‑1 (MQ=255) tGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGGGGAGAAGGGTGCTAATTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGta < 2:2578620/90‑1 (MQ=255) aCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGgaat > 2:1428684/1‑90 (MQ=255) cGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGtaataa < 2:68588/90‑1 (MQ=255) aaGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAAcc < 2:596481/90‑1 (MQ=255) aGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa > 2:335736/1‑45 (MQ=255) aGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 1:335736/45‑1 (MQ=255) ctcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAc > 1:105626/1‑90 (MQ=255) tcCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGAcc > 1:1900318/1‑90 (MQ=255) ccTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGGGCTAAGTATTACCACATCACCCGCCAGTGGCAGGCACGTGGTAATAAACTTACCg > 2:2112691/1‑90 (MQ=255) tATCGGGTTGCGAACCGTGCCGGGGAAAAAGGTGCTAAGTATTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:2023515/90‑1 (MQ=255) tATCGGGTTGCAAAACGTGCGGAAAAAAAAGGTGCTAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 1:2441746/90‑1 (MQ=255) tATCAGGTTGCGAAAGGTGCGGGGGAGAAAGGTGCAAAGTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 1:3006276/90‑1 (MQ=255) tATCAGGTTGCGAAACGTGCCGGGGAGAAAGGTGCTAAGTTTTACCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 1:1631151/90‑1 (MQ=255) tATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc < 2:105626/90‑1 (MQ=255) ggTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAAACTGACCGTCAgcgc > 1:2475798/1‑90 (MQ=255) ggTTGCGAAACGTGCCGCGGAAAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:380991/90‑1 (MQ=255) ggTTGCAAACCGTGCGGGGGAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:1136479/90‑1 (MQ=255) ggTTGCAAACCGGGCCGCGGAAAAGGGTGTTAAGTTTTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 1:1905886/90‑1 (MQ=255) ggTTGCAAAACGGGCCGCGGAGAAAGGTGCTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 1:272425/90‑1 (MQ=255) gTTGCGAAACGTGCTGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa > 1:1136479/1‑90 (MQ=255) gTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGTAATAACCTGACCGTCAGCGCa > 2:1965197/1‑90 (MQ=255) tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGa > 2:2835553/1‑90 (MQ=255) tGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAGCGCAGa > 1:2119443/1‑90 (MQ=255) cGAAAGGTGCCGGGGAAAAAGGTGCAAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:2119443/90‑1 (MQ=255) cGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:804515/90‑1 (MQ=255) cGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:298349/90‑1 (MQ=255) cGAAACGTGCCGCGGAGAAAGGTGCAAAGTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 2:2066872/90‑1 (MQ=255) gAAACGTGCCGCGGAAAAAGGTGCTAATTTTTCCCCCATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCt < 2:884497/90‑1 (MQ=255) tGCCGGGGAAAAAGGTGCAAATTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataa < 2:2994699/90‑1 (MQ=255) cgcgGAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaata < 2:379470/90‑1 (MQ=255) | ATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATA > NZ_CP009273/1673455‑1673627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |