Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,761,086	A→C	R619R (CGT→CGG)	ydiJ ←	FAD‑binding and (Fe‑S)‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,761,086	0	A	C	100.0%	337.8 / NA	89	R619R (CGT→CGG)	ydiJ	FAD‑binding and (Fe‑S)‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/53);  total (36/53)

GGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAC  >  NZ_CP009273/1761001‑1761171                                                                                      |                                                                                      gggCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCa                                                                                   >  2:1130269/1‑90 (MQ=255) gggCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCa                                                                                   >  2:1984443/1‑90 (MQ=255)    cAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGcc                                                                                <  1:2493653/90‑1 (MQ=255)    cAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGcc                                                                                <  2:551802/90‑1 (MQ=255)    cAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGcc                                                                                <  2:2210538/90‑1 (MQ=255)       ttAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCAtt                                                                             <  1:1914165/90‑1 (MQ=255)        tAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTc                                                                            <  1:154196/90‑1 (MQ=255)          aCGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACTGCAGCCATTCAc                                                                          <  2:1024841/90‑1 (MQ=255)          aCGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          <  1:2282458/90‑1 (MQ=255)          aCGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          <  1:706576/90‑1 (MQ=255)            gTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGc                                                                        <  2:1474457/90‑1 (MQ=255)            gTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGc                                                                        <  2:2068722/90‑1 (MQ=255)              ccGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAc                                                                      >  2:2825357/1‑90 (MQ=255)              ccGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAc                                                                      >  1:1193534/1‑90 (MQ=255)              ccGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAc                                                                      >  2:2755057/1‑90 (MQ=255)              ccGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAc                                                                      >  2:802266/1‑90 (MQ=255)              ccGCAAACTGACGCCGGATTCAGGCAATTCTTGTTCCAGTTTGATTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAc                                                                      >  1:2435481/1‑90 (MQ=255)               cGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCAcc                                                                     <  2:3023843/90‑1 (MQ=255)                gCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCa                                                                    >  1:2462858/1‑90 (MQ=255)                  aaaCTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGc                                                                  <  2:2173705/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  2:2598456/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  1:752408/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  2:747691/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  1:313233/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  1:2825357/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  2:2755448/90‑1 (MQ=255)                         cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                           <  1:1984443/90‑1 (MQ=255)                           ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                         <  1:384830/90‑1 (MQ=255)                           ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                         <  1:874407/90‑1 (MQ=255)                           ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                         <  1:769540/90‑1 (MQ=255)                               aTTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCtt                                                     <  1:1626167/90‑1 (MQ=255)                               aTTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCtt                                                     <  1:1450095/90‑1 (MQ=255)                                 tCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTc                                                   <  2:2462858/90‑1 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          <  2:2277685/65‑1 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          >  1:539961/1‑65 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          <  2:539961/65‑1 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCAc                                                                          >  1:2277685/1‑65 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCgg                                                 <  2:1848202/90‑1 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCgg                                                 >  1:2769271/1‑90 (MQ=255)                                   aGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCgg                                                 >  1:1116474/1‑90 (MQ=255)                                        gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaat                                            >  1:1079367/1‑90 (MQ=255)                                        gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaat                                            >  1:2527551/1‑90 (MQ=255)                                        gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaat                                            >  1:2523899/1‑90 (MQ=255)                                        gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaat                                            >  1:2473088/1‑90 (MQ=255)                                        gTTCTTGTTCCAGGTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCCCGCACCAGCGTTGCGCGCCCTTTCGGtgaat                                            >  1:2650232/1‑90 (MQ=255)                                         ttCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaatg                                           <  2:1900659/90‑1 (MQ=255)                                             tGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATc                                       <  1:1101371/90‑1 (MQ=255)                                             tGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATc                                       <  2:2502758/90‑1 (MQ=255)                                              gTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATcc                                      <  2:855860/90‑1 (MQ=255)                                              gTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATcc                                      >  2:107878/1‑90 (MQ=255)                                                tCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCgg                                    >  2:75617/1‑90 (MQ=255)                                                  cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                                  >  2:885493/1‑90 (MQ=255)                                                  cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                                  >  2:2337328/1‑90 (MQ=255)                                                  cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                                  >  1:2395868/1‑90 (MQ=255)                                                  cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                                  >  2:2943201/1‑90 (MQ=255)                                                  cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                                  >  2:852435/1‑90 (MQ=255)                                                   aGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc                                 <  1:2745227/90‑1 (MQ=255)                                                   aGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc                                 >  1:668331/1‑90 (MQ=255)                                                     tttGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTg                               <  2:2523899/90‑1 (MQ=255)                                                     tttGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTg                               <  2:1079367/90‑1 (MQ=255)                                                        gAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGt                            >  1:1635056/1‑90 (MQ=255)                                                          gTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGa                          <  2:2287996/90‑1 (MQ=255)                                                            ggATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATc                        >  2:873906/1‑90 (MQ=255)                                                             gATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc                                 <  1:1330135/80‑1 (MQ=255)                                                             gATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTc                                 >  2:1330135/1‑80 (MQ=255)                                                               tCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTc                     <  2:335196/90‑1 (MQ=255)                                                                 aaCGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCAt                   <  1:1029993/90‑1 (MQ=255)                                                                  aCGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATc                  >  1:2972339/1‑90 (MQ=255)                                                                  aCGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGGGAATGAATCCGGTTCTGGGTGATCTTCATc                  >  1:2215672/1‑90 (MQ=255)                                                                   cgccgcGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCg                 <  1:1129344/90‑1 (MQ=255)                                                                    gccgcGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGa                <  1:542940/90‑1 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                         >  2:1596159/1‑46 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                         <  1:1596159/46‑1 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACgg             >  1:749319/1‑90 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACgg             >  1:722963/1‑90 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACgg             >  2:524145/1‑90 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACgg             >  1:1532391/1‑90 (MQ=255)                                                                       gcgATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACgg             >  2:1999387/1‑90 (MQ=255)                                                                         gATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGac           <  2:1864876/90‑1 (MQ=255)                                                                         gATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGac           <  1:622959/90‑1 (MQ=255)                                                                         gATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGac           <  1:2738244/90‑1 (MQ=255)                                                                         gATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGac           <  2:2002515/90‑1 (MQ=255)                                                                             cGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt       <  1:1630544/90‑1 (MQ=255)                                                                             cGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACAt       <  2:2648168/90‑1 (MQ=255)                                                                              gCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc      <  2:1949229/90‑1 (MQ=255)                                                                              gCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc      <  1:614741/90‑1 (MQ=255)                                                                              gCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATc      <  1:885493/90‑1 (MQ=255)                                                                                caacaaCCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCgg    <  2:2695407/90‑1 (MQ=255)                                                                                  acaaCCGCAGCCATTCACGCACCAGCGATGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAc  <  1:740264/90‑1 (MQ=255)                                                                                      |                                                                                      GGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGAC  >  NZ_CP009273/1761001‑1761171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑