Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,761,952	G→C	H331D (CAT→GAT)	ydiJ ←	FAD‑binding and (Fe‑S)‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,761,952	0	G	C	100.0%	331.5 / NA	87	H331D (CAT→GAT)	ydiJ	FAD‑binding and (Fe‑S)‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (45/42);  total (45/42)

GCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGG  >  NZ_CP009273/1761865‑1762036                                                                                        |                                                                                     gcgcCTCATCATCACCAGCAAATTCCCTAAGGTTCAGGCCGGGCAACTTTTGGTAAGGCACATCGGTAATCAACTCGCTGACGGAATccc                                                                                    <  1:2378311/90‑1 (MQ=255) gcgcCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATccc                                                                                    <  1:788711/90‑1 (MQ=255)  cgcCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCa                                                                                   >  1:2293297/1‑90 (MQ=255)  cgcCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCa                                                                                   >  2:1534666/1‑90 (MQ=255)   gcCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAg                                                                                  <  2:900385/90‑1 (MQ=255)    ccTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGa                                                                                 <  2:2720844/90‑1 (MQ=255)    ccTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGa                                                                                 >  1:630628/1‑90 (MQ=255)     cTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGAc                                                                                <  1:998757/90‑1 (MQ=255)     cTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGAc                                                                                <  2:786292/90‑1 (MQ=255)       catcatcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACag                                                                              >  2:896470/1‑89 (MQ=255)       catcatcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACaa                                                                              >  2:2400729/1‑90 (MQ=255)       catcatcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACaa                                                                              >  1:619815/1‑90 (MQ=255)       catcatcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACaa                                                                              >  1:442123/1‑90 (MQ=255)         tcatcaCCAGCAAATTCCACAATGTTTAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAata                                                                            >  2:1045864/1‑90 (MQ=255)          catcaCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAatat                                                                           <  2:570919/90‑1 (MQ=255)             caCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCtt                                                                        >  1:1840785/1‑90 (MQ=255)              aCCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTc                                                                       >  1:2608839/1‑90 (MQ=255)                 aGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCcg                                                                    >  2:1129686/1‑90 (MQ=255)                 aGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCcg                                                                    >  1:1136148/1‑90 (MQ=255)                  gCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCcgc                                                                   >  1:1509708/1‑90 (MQ=255)                     aaTTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGcc                                                                >  2:2355424/1‑90 (MQ=255)                      aTTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCa                                                               <  2:2933659/90‑1 (MQ=255)                       ttCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAg                                                              >  2:550240/1‑90 (MQ=255)                         ccACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGAt                                                            >  2:2795063/1‑90 (MQ=255)                           acaATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTc                                                          <  1:1818002/90‑1 (MQ=255)                           acaATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTc                                                          <  2:1840785/90‑1 (MQ=255)                            caATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCa                                                         >  2:775405/1‑90 (MQ=255)                             aaTGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAg                                                        <  2:1361680/90‑1 (MQ=255)                              aTGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGc                                                       >  2:1818636/1‑90 (MQ=255)                              aTGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGc                                                       <  2:1685466/90‑1 (MQ=255)                                gTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCAc                                                     <  1:1393314/90‑1 (MQ=255)                                gTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCAc                                                     >  2:1565137/1‑90 (MQ=255)                                gTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCAc                                                     >  1:2149838/1‑90 (MQ=255)                                  tCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACtt                                                   <  2:31020/90‑1 (MQ=255)                                   cAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACttt                                                  >  2:1368269/1‑90 (MQ=255)                                   cAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACttt                                                  >  2:2755818/1‑90 (MQ=255)                                        cGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGt                                             >  2:1786182/1‑90 (MQ=255)                                        cGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGt                                             >  1:2325032/1‑90 (MQ=255)                                         gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTc                                            <  2:2854978/90‑1 (MQ=255)                                         gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTc                                            >  1:2451242/1‑90 (MQ=255)                                         gAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTc                                            >  1:1400159/1‑90 (MQ=255)                                          aGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTcc                                           >  1:595863/1‑90 (MQ=255)                                          aGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTcc                                           >  1:559043/1‑90 (MQ=255)                                          aGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTcc                                           >  2:1325761/1‑90 (MQ=255)                                           gCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCa                                          <  2:293407/90‑1 (MQ=255)                                            cATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAc                                         <  1:1799623/90‑1 (MQ=255)                                            cATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAc                                         <  1:1234898/90‑1 (MQ=255)                                             aTCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAcc                                        <  1:1138526/90‑1 (MQ=255)                                             aTCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAcc                                        <  2:2293297/90‑1 (MQ=255)                                             aTCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAcc                                        <  1:2795063/90‑1 (MQ=255)                                             aTCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAcc                                        <  1:627086/90‑1 (MQ=255)                                             aTCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCAcc                                        <  1:820471/90‑1 (MQ=255)                                               ctctTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCAc                                                     <  1:100132/75‑1 (MQ=255)                                               ctctTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCAc                                                     >  2:100132/1‑75 (MQ=255)                                                 ctTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGtct                                    <  1:1684099/90‑1 (MQ=255)                                                   tGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTctct                                  >  2:1448395/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATccc                                                                                    <  2:1503004/35‑1 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  1:1992917/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  1:521243/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  1:862852/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  2:844660/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  1:894223/1‑90 (MQ=255)                                                        aGGCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGa                             >  1:293311/1‑90 (MQ=255)                                                         ggCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGaa                            >  1:581793/1‑90 (MQ=255)                                                         ggCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGaa                            >  1:2436975/1‑90 (MQ=255)                                                         ggCACATCGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGaa                            >  2:1476489/1‑90 (MQ=255)                                                                cGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGccc                     <  2:2957696/90‑1 (MQ=255)                                                                cGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGccc                     <  2:2325032/90‑1 (MQ=255)                                                                cGGTAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGccc                     <  2:1400159/90‑1 (MQ=255)                                                                   tAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCa                                          <  1:2418433/66‑1 (MQ=255)                                                                   tAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCa                                          >  2:2418433/1‑66 (MQ=255)                                                                   tAATCAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCcgcg                  >  2:936102/1‑90 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:2451242/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  1:2400729/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1874801/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1722766/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1562372/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1509708/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1436031/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  2:1186203/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  1:936102/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  1:2135254/90‑1 (MQ=255)                                                                       cAACTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTc              <  1:2258651/90‑1 (MQ=255)                                                                        aaCTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCa             <  2:894223/90‑1 (MQ=255)                                                                         aCTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCaa            >  1:2998543/1‑90 (MQ=255)                                                                          cTCGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAAc           >  1:949301/1‑90 (MQ=255)                                                                            cGCTGACGGAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCa         >  1:344196/1‑90 (MQ=255)                                                                                   ggAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACgg  <  1:2355424/90‑1 (MQ=255)                                                                                   ggAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACgg  <  1:1476489/90‑1 (MQ=255)                                                                                   ggAATCCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACgg  <  2:2755319/90‑1 (MQ=255)                                                                                        |                                                                                     GCGCCTCATCATCACCAGCAAATTCCACAATGTTCAGCCCGAGCATCTCTTGGTCAGGCACATCGGTAATCAACTCGCTGACGGAATGCCAGACAATATCTTCCCGCGCCAGATTCAGCACTTTTGAGTCCACCGTCTCTACCGAAAGCGCCCGCGCCTCAACCATAAACGG  >  NZ_CP009273/1761865‑1762036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑