Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,848,011	T→C	T76A (ACT→GCT)	ydjE ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,848,011	0	T	C	100.0%	253.5 / NA	67	T76A (ACT→GCT)	ydjE	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/37);  total (30/37)

GGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCA  >  NZ_CP009273/1847925‑1848094                                                                                       |                                                                                    ggCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAg                                                                                  >  1:178210/1‑90 (MQ=255)   ccccTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTg                                                                                <  1:2280118/90‑1 (MQ=255)   ccccTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTg                                                                                <  1:1530788/90‑1 (MQ=255)     ccTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAg                                                                              <  2:1320428/90‑1 (MQ=255)      cTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGc                                                                             >  2:893481/1‑90 (MQ=255)        gTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCAGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCg                                                                           <  1:1457326/90‑1 (MQ=255)                aaTACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATa                                                                   >  2:2863846/1‑90 (MQ=255)                 aTACCGACGATGAGAAGATTTATGGGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATaa                                                                  >  1:1780230/1‑90 (MQ=255)                     cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCaa                                                              <  1:1490628/90‑1 (MQ=255)                     cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCaa                                                              <  2:1461870/90‑1 (MQ=255)                     cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCaa                                                              <  1:842845/90‑1 (MQ=255)                     cgacgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCaa                                                              <  1:1252722/90‑1 (MQ=255)                        cgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACa                                                           >  1:1617583/1‑90 (MQ=255)                        cgaTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACa                                                           >  1:164899/1‑90 (MQ=255)                          aTGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACAtt                                                         <  2:454424/90‑1 (MQ=255)                                agaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGcc                                                   <  2:2419333/90‑1 (MQ=255)                                 gaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCg                                                  <  1:2832294/90‑1 (MQ=255)                                 gaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCg                                                  <  1:2425880/90‑1 (MQ=255)                                 gaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCg                                                  <  1:2813816/90‑1 (MQ=255)                                 gaTTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCg                                                  <  1:167886/90‑1 (MQ=255)                                      aTGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTa                                             <  1:2415182/90‑1 (MQ=255)                                           aaaCGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAggc                                        >  1:2836329/1‑90 (MQ=255)                                                   tGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACAtt                                                         >  1:1339659/1‑65 (MQ=255)                                                   tGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACAtt                                                         <  2:1339659/65‑1 (MQ=255)                                                    gcgcCGCCCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAg                               >  1:309463/1‑90 (MQ=255)                                                       ccgccCAAAGTAGTCACCAATAAACCCCCCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            <  1:16896/90‑1 (MQ=255)                                                       ccgccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            <  2:2084539/90‑1 (MQ=255)                                                       ccgccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            <  1:2040637/90‑1 (MQ=255)                                                       ccgccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            <  2:2632008/90‑1 (MQ=255)                                                        cgccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaaa                           <  2:1690174/90‑1 (MQ=255)                                                         tcccAAAGTAGTCTCCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGGAAAGGCGGCATTGAGGAAat                          >  1:2120100/2‑90 (MQ=255)                                                         gccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAat                          >  1:956694/1‑90 (MQ=255)                                                         gccCAAAGTAGTCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAat                          >  1:2579334/1‑90 (MQ=255)                                                                 tagtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  <  2:956694/90‑1 (MQ=255)                                                                 tagtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  <  1:2817702/90‑1 (MQ=255)                                                                 tagtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  <  2:2426135/90‑1 (MQ=255)                                                                 tagtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  <  2:195030/90‑1 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                <  2:2417088/90‑1 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  1:616193/1‑90 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  1:2213571/1‑90 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  2:1903414/1‑90 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  2:1706802/1‑90 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                <  2:1651786/90‑1 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  1:1651786/1‑90 (MQ=255)                                                                   gtCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  2:2701845/1‑90 (MQ=255)                                                                    tCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccat               >  2:249022/1‑90 (MQ=255)                                                                    tCACCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccat               >  2:1250196/1‑90 (MQ=255)                                                                      aCCAATAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAccatcc             <  2:1277714/90‑1 (MQ=255)                                                                         aaTAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCAtt          <  1:249022/90‑1 (MQ=255)                                                                         aaTAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCAtt          <  2:2025729/90‑1 (MQ=255)                                                                         aaTAAACCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCAtt          <  1:1250196/90‑1 (MQ=255)                                                                             aaCCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                <  1:893830/80‑1 (MQ=255)                                                                             aaCCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAcca                >  2:893830/1‑80 (MQ=255)                                                                             aaCCCACCAGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCtt      >  2:478185/1‑90 (MQ=255)                                                                                ccaccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTAcc   <  2:2572649/90‑1 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTa                                             <  2:2015864/47‑1 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTa                                             >  1:2015864/1‑47 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            >  1:97702/1‑64 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGaa                            <  2:97702/64‑1 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTAttt                     <  2:2230409/71‑1 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTAttt                     >  1:2230409/1‑71 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  >  2:2242177/1‑74 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGAc                  <  1:2242177/74‑1 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCa  >  1:927132/1‑90 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCa  >  1:2782727/1‑90 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCa  >  2:2865415/1‑90 (MQ=255)                                                                                 caccaGCAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCa  >  1:2105572/1‑90 (MQ=255)                                                                                       |                                                                                    GGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTCACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCA  >  NZ_CP009273/1847925‑1848094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑