Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,900,885	A→G	S145P (TCC→CCC)	rlmA ←	23S rRNA (guanine(745)‑N(1))‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,900,885	0	A	G	100.0%	307.0 / NA	81	S145P (TCC→CCC)	rlmA	23S rRNA (guanine(745)‑N(1))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/23);  total (58/23)

CCCAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAAC  >  NZ_CP009273/1900798‑1900969                                                                                        |                                                                                     cccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaa                                                                                    >  1:879119/1‑90 (MQ=255)  ccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaaa                                                                                   <  1:1072299/90‑1 (MQ=255)  ccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaaa                                                                                   <  1:2303063/90‑1 (MQ=255)  ccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaaa                                                                                   <  2:482745/90‑1 (MQ=255)  ccAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGgaaa                                                                                   <  1:143901/90‑1 (MQ=255)    aGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACg                                                                                 >  1:1609963/1‑90 (MQ=255)    aGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACg                                                                                 >  2:23173/1‑90 (MQ=255)    aGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACg                                                                                 >  2:2694476/1‑90 (MQ=255)         ccgGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCaaa                                                                            >  2:65696/1‑90 (MQ=255)                      ctCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAg                                                               >  2:2561793/1‑90 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             <  1:1010095/90‑1 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             >  2:1838426/1‑90 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             <  1:2312595/90‑1 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             >  2:797630/1‑90 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             <  2:211751/90‑1 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             <  1:2098464/90‑1 (MQ=255)                        cGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             >  1:2001949/1‑90 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             >  2:1826064/1‑88 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCg                                                             <  1:1826064/88‑1 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  2:1478600/1‑90 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  1:727333/1‑90 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  1:133673/1‑90 (MQ=255)                          tGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  2:2924228/1‑90 (MQ=255)                             tAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                        <  2:1609963/90‑1 (MQ=255)                               aTTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACaaa                                                      >  2:155361/1‑90 (MQ=255)                                    tctGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTg                                                 >  1:1973171/1‑90 (MQ=255)                                       gCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGAcc                                              <  1:2965633/90‑1 (MQ=255)                                        cTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCt                                             <  2:169350/90‑1 (MQ=255)                                         tttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTg                                            <  1:432635/90‑1 (MQ=255)                                          ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGc                                           >  1:2046512/1‑90 (MQ=255)                                          ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGc                                           >  2:2558084/1‑90 (MQ=255)                                            acacGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCgg                                         >  2:1600872/1‑90 (MQ=255)                                              acGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGAt                                       <  1:1623373/90‑1 (MQ=255)                                               cGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATa                                      >  1:550356/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCaa                                                                             >  2:1081095/1‑49 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCaa                                                                             <  1:1081095/49‑1 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  2:903878/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  2:1187589/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  2:1839506/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  2:2105582/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  1:1353506/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  1:1483813/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  1:2139172/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  1:2769069/1‑90 (MQ=255)                                                 gcgcGTAAATACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGc                                    >  1:325291/1‑90 (MQ=255)                                                        aaTACGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgc                             <  1:2105582/90‑1 (MQ=255)                                                           aCGTATTATGGCGTCCGTACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  1:1746324/1‑57 (MQ=255)                                                           aCGTATTATGGCGTCCGTACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           <  2:1746324/57‑1 (MQ=255)                                                           aCGTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgc                          >  1:2018816/1‑90 (MQ=255)                                                             gTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           >  1:1719338/1‑55 (MQ=255)                                                             gTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGac                                                           <  2:1719338/55‑1 (MQ=255)                                                             gTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgccg                        >  2:1984589/1‑90 (MQ=255)                                                             gTATTATGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgccg                        >  2:1782700/1‑90 (MQ=255)                                                              tattatGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTcgccgccgc                       >  2:1118382/1‑90 (MQ=255)                                                               attatGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCt                      >  2:815270/1‑90 (MQ=255)                                                                ttatGGCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCtt                     >  1:563698/1‑90 (MQ=255)                                                                    ggCGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGGGACCTGCGGATAGCGTTTCGCCGCCGCTTTTAt                 >  2:1348468/1‑90 (MQ=255)                                                                      cGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCg               >  2:1020663/1‑90 (MQ=255)                                                                      cGTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCg               >  2:3007842/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                        >  1:25400/1‑48 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGAcaca                                                        <  2:25400/48‑1 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  2:2320208/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  2:1083177/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  1:45967/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  2:1357454/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  2:1839264/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  1:3002450/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              <  1:2657555/90‑1 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  1:2211581/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  1:9435/1‑90 (MQ=255)                                                                       gTCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGGGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGc              >  1:1387233/1‑90 (MQ=255)                                                                        tCCATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCt             <  2:229587/90‑1 (MQ=255)                                                                         ccATACTGGTATCGGGAAACGGCAATCGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTa            <  2:259821/90‑1 (MQ=255)                                                                         ccATACTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTa            >  2:801361/1‑90 (MQ=255)                                                                             aCTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt        <  2:187294/90‑1 (MQ=255)                                                                             aCTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt        <  1:2227552/90‑1 (MQ=255)                                                                              cTGGTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTc       >  2:2950802/1‑90 (MQ=255)                                                                                ggTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTg                                            <  2:351771/51‑1 (MQ=255)                                                                                ggTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTg                                            >  1:351771/1‑51 (MQ=255)                                                                                ggTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa     >  2:366715/1‑90 (MQ=255)                                                                                 gTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa    <  1:2924228/90‑1 (MQ=255)                                                                                 gTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa    >  1:3022130/1‑90 (MQ=255)                                                                                 gTATCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa    >  2:2198198/1‑90 (MQ=255)                                                                                   aTCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAAc  <  2:1973171/90‑1 (MQ=255)                                                                                   aTCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAAc  >  1:210001/1‑90 (MQ=255)                                                                                   aTCGGGAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAAc  >  2:1367690/1‑90 (MQ=255)                                                                                        |                                                                                     CCCAGCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAAC  >  NZ_CP009273/1900798‑1900969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑