Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,941,604	A→C	G10G (GGT→GGG)	ruvC ←	crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,941,604	0	A	C	100.0%	317.9 / NA	85	G10G (GGT→GGG)	ruvC	crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (44/41);  total (44/41)

CCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCT  >  NZ_CP009273/1941519‑1941687                                                                                      |                                                                                    ccACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCg                                                                                 >  1:711633/1‑90 (MQ=255)  cACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCgg                                                                                <  1:623355/90‑1 (MQ=255)    cTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGAt                                                                              >  1:477116/1‑90 (MQ=255)      ttGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCa                                                                            >  1:464467/1‑90 (MQ=255)            cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  1:599201/90‑1 (MQ=255)            cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  2:2177108/90‑1 (MQ=255)            cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  2:2133904/90‑1 (MQ=255)            cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  2:1849915/90‑1 (MQ=255)            cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  2:1392261/90‑1 (MQ=255)              gATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCgag                                                                    >  1:2357967/1‑90 (MQ=255)              gATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCgag                                                                    <  2:1005648/90‑1 (MQ=255)              gATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCgag                                                                    >  1:1963228/1‑90 (MQ=255)              gATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCgag                                                                    >  2:1079301/1‑90 (MQ=255)                  cATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGGGaata                                                                >  1:1916870/1‑90 (MQ=255)                  cATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGaata                                                                >  1:375460/1‑90 (MQ=255)                     ccGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAataata                                                             <  1:2299207/90‑1 (MQ=255)                       gCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGc                                                           >  1:2783628/1‑90 (MQ=255)                           cccAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATc                                                       <  2:1816588/90‑1 (MQ=255)                           cccAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATc                                                       <  1:1929875/90‑1 (MQ=255)                           cccAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATc                                                       <  1:2735068/90‑1 (MQ=255)                               ggTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgc                                                   >  1:2600102/1‑90 (MQ=255)                               ggTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgc                                                   <  1:1317942/90‑1 (MQ=255)                               ggTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgc                                                   <  2:505540/90‑1 (MQ=255)                                gTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgcg                                                  >  1:1470987/1‑90 (MQ=255)                                    gACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtctc                                              >  2:2622998/1‑90 (MQ=255)                                    gACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtctc                                              >  2:69557/1‑90 (MQ=255)                                    gACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtctc                                              >  2:54316/1‑90 (MQ=255)                                       aGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGt                                           <  1:2614035/90‑1 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  1:2085333/62‑1 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  1:788256/62‑1 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      >  2:788256/1‑62 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      >  2:2085333/1‑62 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                          >  1:1024026/1‑90 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                          >  2:1369964/1‑90 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                          >  2:1494431/1‑90 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                          >  1:1016439/1‑90 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                          >  1:1120471/1‑90 (MQ=255)                                        gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGCCTCCGtt                                          >  1:1375845/1‑90 (MQ=255)                                         ttGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtctc                                              <  1:1181261/85‑1 (MQ=255)                                         ttGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtctc                                              >  2:1181261/1‑85 (MQ=255)                                         ttGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGttt                                         >  1:2694656/1‑90 (MQ=255)                                         ttGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGttt                                         >  2:1992953/1‑90 (MQ=255)                                            cctacctaCCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGc                                      <  2:2954883/90‑1 (MQ=255)                                             ctacctacCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  1:111378/57‑1 (MQ=255)                                             ctacctacCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      >  2:111378/1‑57 (MQ=255)                                             ctacctacCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCt                                     >  1:1149454/1‑90 (MQ=255)                                              tacctaccTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTg                                    >  2:2503438/1‑90 (MQ=255)                                              tacctaccTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTg                                    <  1:1306007/90‑1 (MQ=255)                                               acctacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGt                                   <  1:2951146/90‑1 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      >  1:1407634/1‑52 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  2:1407634/52‑1 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  2:554804/1‑90 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  1:801952/1‑90 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  1:926689/1‑90 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  1:2664211/1‑90 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  2:819802/1‑90 (MQ=255)                                                  tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa                                >  1:1556930/1‑90 (MQ=255)                                                    cctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGc                              <  2:2108967/90‑1 (MQ=255)                                                    cctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGc                              <  1:713812/90‑1 (MQ=255)                                                    cctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGc                              <  2:2600102/90‑1 (MQ=255)                                                      tGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAg                            <  1:821517/90‑1 (MQ=255)                                                       ggCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      <  1:1828607/47‑1 (MQ=255)                                                       ggCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                      >  2:1828607/1‑47 (MQ=255)                                                        gCGGATGACGCCGTAGCCGGTCACGCGCGCCCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc                          <  1:1521783/90‑1 (MQ=255)                                                        gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc                          <  2:1470987/90‑1 (MQ=255)                                                        gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc                          <  1:1871112/90‑1 (MQ=255)                                                        gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc                          <  1:923472/90‑1 (MQ=255)                                                         cGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGcc                         >  2:2776312/1‑90 (MQ=255)                                                          ggATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCt                        >  2:975572/1‑90 (MQ=255)                                                          ggATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCt                        >  2:1616179/1‑90 (MQ=255)                                                           gATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCtc                       <  2:1512719/90‑1 (MQ=255)                                                              gACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAACAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCtcatc                    >  1:1549685/1‑90 (MQ=255)                                                                cGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCag                  <  2:2500626/90‑1 (MQ=255)                                                                cGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCag                  <  1:2241405/90‑1 (MQ=255)                                                                  ccGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGc                                      <  2:2220322/68‑1 (MQ=255)                                                                  ccGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGc                                      >  1:2220322/1‑68 (MQ=255)                                                                     tAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGt             <  2:430553/90‑1 (MQ=255)                                                                          ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg        <  2:2335266/90‑1 (MQ=255)                                                                          ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg        >  2:3000445/1‑90 (MQ=255)                                                                          ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg        <  1:2321555/90‑1 (MQ=255)                                                                           gTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGc       >  1:1248996/1‑90 (MQ=255)                                                                               cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc   <  2:2184639/90‑1 (MQ=255)                                                                               cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc   <  1:1992953/90‑1 (MQ=255)                                                                               cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc   <  2:682139/90‑1 (MQ=255)                                                                                gcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTCGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCt  >  1:2049774/1‑90 (MQ=255)                                                                                      |                                                                                    CCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCT  >  NZ_CP009273/1941519‑1941687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑