| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,966,986 | G→C | intergenic (‑38/+107) | tar ← / ← cheW | methyl‑accepting chemotaxis protein II/chemotaxis protein CheW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,966,986 | 0 | G | C | 97.6% | 275.3 / NA | 83 | intergenic (‑38/+107) | tar/cheW | methyl‑accepting chemotaxis protein II/chemotaxis protein CheW |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (49/31); total (49/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAT > NZ_CP009273/1966899‑1967069 | aCCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCa < 2:2457775/90‑1 (MQ=255) ccccAGCACCATTACCAACAGGGGGACTAGCCGGTTACGGTTAATCATAAGGCCCCTTCCGGAAAAAAAGTTGTTCTCGTTATCGCCagg > 2:2887668/1‑88 (MQ=255) ccccAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAag > 2:1464276/1‑90 (MQ=255) ccccAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAag > 1:200554/1‑90 (MQ=255) ccAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAagga > 1:2409580/1‑90 (MQ=255) cAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAaggag < 1:2151549/90‑1 (MQ=255) gCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAggg > 2:2218954/1‑90 (MQ=255) ccATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAgggggg > 1:2960530/1‑90 (MQ=255) cATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAaggag < 2:2690159/84‑1 (MQ=255) cATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAaggag > 1:2690159/1‑84 (MQ=255) cATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGa > 1:1242831/1‑90 (MQ=255) cATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGa > 2:2085447/1‑90 (MQ=255) cATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGa > 2:2542283/1‑90 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:2575918/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:354042/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa > 1:3018020/1‑90 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:479521/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 2:2576254/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 2:69535/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:1935304/90‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa > 2:61936/1‑90 (MQ=255) cAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑ttt > 2:259806/1‑90 (MQ=255) cAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑ttt > 2:544247/1‑90 (MQ=255) aaCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTa > 2:143450/1‑90 (MQ=255) aaCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTa > 1:1150722/1‑90 (MQ=255) aCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTAt < 1:2733839/90‑1 (MQ=255) aCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTAt < 2:1072990/90‑1 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAaggag < 2:1923096/75‑1 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAaggag > 1:1923096/1‑75 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa > 2:1818924/1‑82 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa > 2:1640001/1‑82 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:1818924/82‑1 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa > 1:571822/1‑82 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 1:1640001/82‑1 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGaa < 2:571822/82‑1 (MQ=255) cAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTAtt > 1:2300130/1‑90 (MQ=255) gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑Gc > 2:2057987/1‑90 (MQ=255) gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑Gc > 2:524939/1‑90 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCt < 2:1749456/90‑1 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCt < 2:1186487/90‑1 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCt < 1:982045/90‑1 (MQ=255) tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGa > 1:46068/1‑90 (MQ=255) aCTACGCGGATACGGTTAATCAAAAGCCCCCTTCCTAAAAAAAAGTTGATCTCGTTATCGCAAAGGAGGGGGGAAAC‑TTTATT‑GCTGATg < 1:2160583/90‑1 (MQ=255) aCGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCa > 1:1058073/1‑90 (MQ=255) aCGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGA‑GGGGGAAAC‑TTTATT‑GCTGATGCCAc > 1:1838678/1‑90 (MQ=255) gcgGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCAcc > 2:634770/1‑90 (MQ=255) gcgGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCAcc > 1:765725/1‑90 (MQ=255) gcgGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCAcc > 2:1479955/1‑90 (MQ=255) aTACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACccgcc > 1:2429644/1‑90 (MQ=255) gTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGaa > 1:2917586/1‑90 (MQ=255) aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAAtt > 2:2002404/1‑90 (MQ=255) aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAAtt > 2:2495827/1‑90 (MQ=255) tAAGGCACCTTCCTGAAAACTAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAAt > 2:309368/1‑90 (MQ=255) tAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAAt > 2:393355/1‑90 (MQ=255) tAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAAt > 1:263002/1‑90 (MQ=255) aGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATaa > 1:562486/1‑90 (MQ=255) aGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATaa > 1:2844221/1‑90 (MQ=255) ggCACCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATaaa < 2:2300130/90‑1 (MQ=255) aCCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATaaaaaa > 2:712622/1‑90 (MQ=255) aCCTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATaaaaaa > 1:2677190/1‑90 (MQ=255) ccTTCCTGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAAc > 2:732451/1‑90 (MQ=255) ttCCTAAAAACAAGTTGATCTCTTTATC‑GCAAAGGGGGGGGAAACTTTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAAccc < 2:2967371/90‑1 (MQ=255) ttCCTAAAAACAAGTTGATCTCGTTACCGCAAAGGGGGGGGAAAAC‑TTTTTT‑GCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAAccc < 1:61936/90‑1 (MQ=255) ccTAAAAACAAGTTGATCTCTTTACCGCCAGGGGGGGGGGAAAT‑TTTTTT‑GCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGa < 2:2773018/90‑1 (MQ=255) ccTAAAAAAAAGTTGCTCTCTTTACCGCGAGGGGGGGGGAAAAC‑TTTATT‑CCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGa < 1:2896393/90‑1 (MQ=255) taaaaaCAAGTTAATCTCGTTACCGCCAAGGAGGGGGAAAAC‑TTTATT‑GCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATg < 2:2429644/88‑1 (MQ=255) gAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATgc > 1:1733508/1‑90 (MQ=255) gAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATgc > 1:1769325/1‑90 (MQ=255) gAAAAAAAGTTGATCCCTTACGCGCCAAGGGGGGGGAAAAT‑TTTATT‑GCGGAACCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATgc < 1:1464276/90‑1 (MQ=255) aaaCAAGTTCATCCCGTTA‑CCGCAAAGGGGGGGGAAAT‑TTTTTTGGCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATgcgc < 1:1741409/90‑1 (MQ=255) aaCAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCa > 2:1400567/1‑90 (MQ=255) aaCAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCa > 1:1820343/1‑90 (MQ=255) aaCAAGTTGATCTCGTTATCGCAAAGGAGGGGGAAAAT‑TTTATT‑GCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCa < 1:524939/90‑1 (MQ=255) aaCAAGTGGATTTCGTTACCGCCAGGGAGGGGGAAAAT‑TTTATT‑GCGGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCa < 1:1659610/90‑1 (MQ=255) aaaaGTTAATCTCGTTATCGCCAGGGGGGGGGAAAAT‑TTTTTT‑GCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAg < 1:934514/88‑1 (MQ=255) aTCTCGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCgg > 1:2812486/1‑90 (MQ=255) ctctTTATCGCCAGGGGGGGGGGAATT‑TTTATT‑GCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGt < 2:1500411/90‑1 (MQ=255) ctcGTTATCGCCAGGGGGGGGGAAATC‑TTTTTT‑GCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGt < 2:2443735/90‑1 (MQ=255) ctcGTTATCGCAAAGGGGGGGGGAAAC‑TTTATT‑GCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGt < 2:434376/90‑1 (MQ=255) ctcGTTATCGAAAGGGAGGGGGAAATC‑TTTTTT‑GCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGt < 1:2972672/90‑1 (MQ=255) tcGTTATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGtt > 2:1817459/1‑90 (MQ=255) ttATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAt > 2:47377/1‑90 (MQ=255) ttATCGCCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAt > 1:1913245/1‑90 (MQ=255) | ACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAAC‑TTTATT‑GCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAT > NZ_CP009273/1966899‑1967069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |