Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,980,420	A→G	G5G (GGA→GGG)	ftnB →	non‑heme ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,980,420	0	A	G	100.0%	284.7 / NA	76	G5G (GGA→GGG)	ftnB	non‑heme ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/28);  total (48/28)

CAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACA  >  NZ_CP009273/1980343‑1980506                                                                              |                                                                                       cAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCaaa                                                                            >  2:1561968/1‑90 (MQ=255)    tAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactc                                                                         >  1:2763631/1‑90 (MQ=255)       ttATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactcaac                                                                      <  1:952775/90‑1 (MQ=255)         aTGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactcaactc                                                                    <  2:2763631/90‑1 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCa                                                                              >  1:687909/1‑78 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCa                                                                              <  2:687909/78‑1 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  1:2062888/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  1:648396/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  1:788663/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  1:562407/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  2:2429430/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  2:2441377/1‑90 (MQ=255)           ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                                  >  1:125078/1‑90 (MQ=255)             tgtTTTGATTTATCTTGAACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCaa                                                                >  2:1120499/1‑90 (MQ=255)                tttGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATg                                                             >  1:2778045/1‑90 (MQ=255)                   gATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAAc                                                          >  1:218870/1‑90 (MQ=255)                    aTTTATCTTGAACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAAcc                                                         <  1:1120499/90‑1 (MQ=255)                       tATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACcgcg                                                      <  1:953869/90‑1 (MQ=255)                       tATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACcgcg                                                      <  2:2411736/90‑1 (MQ=255)                        aTCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGa                                                     >  1:737546/1‑90 (MQ=255)                        aTCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGa                                                     >  2:2253496/1‑90 (MQ=255)                             gCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGtttt                                                >  1:1167004/1‑90 (MQ=255)                               aCCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTAc                                              <  2:2778045/90‑1 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTAc                                              >  1:1649365/1‑87 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTAc                                              <  2:1649365/87‑1 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  1:1300690/1‑90 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  2:2891402/1‑90 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  2:1383108/1‑90 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  2:2043326/1‑90 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  1:1559094/1‑90 (MQ=255)                                  tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                           >  2:2471965/1‑90 (MQ=255)                                    tcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATc                                         <  1:180431/90‑1 (MQ=255)                                     ccACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATcc                                        <  2:1559094/90‑1 (MQ=255)                                     ccACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATcc                                        <  1:1921403/90‑1 (MQ=255)                                       aCTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactc                                                                         <  2:1202740/55‑1 (MQ=255)                                       aCTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactc                                                                         >  1:1202740/1‑55 (MQ=255)                                       aCTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCaa                                      >  1:755619/1‑90 (MQ=255)                                          tCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtct                                   >  2:616477/1‑90 (MQ=255)                                           cTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtctc                                  <  1:1512259/90‑1 (MQ=255)                                             ggATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGGGTTTTACGCATCCAATCTCTa                                >  1:1879442/1‑90 (MQ=255)                                             ggATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa                                >  1:68362/1‑90 (MQ=255)                                               atatAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTAcc                              >  1:106007/1‑90 (MQ=255)                                               atatAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTAcc                              >  2:249118/1‑90 (MQ=255)                                                tataAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCt                             <  2:1769548/90‑1 (MQ=255)                                                  taAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTc                           >  1:2329755/1‑90 (MQ=255)                                                  taAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTc                           >  1:2710071/1‑90 (MQ=255)                                                  taAGGATATTAGGTATGGCAACCGCTGGGATCCTTCTCAAACTCAACTCTCAAATGACCCGGGGGTTTTACCCATCCAATCTCTACCTTc                           >  2:1932351/1‑90 (MQ=255)                                                   aaGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCa                          <  1:2429430/90‑1 (MQ=255)                                                   aaGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCa                          <  1:249118/90‑1 (MQ=255)                                                       atatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTg                      >  1:2383838/1‑90 (MQ=255)                                                       atatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTg                      >  1:1272950/1‑90 (MQ=255)                                                        tatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCt                       >  1:2110819/1‑88 (MQ=255)                                                        tatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACGCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCt                       <  2:2110819/88‑1 (MQ=255)                                                         atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa                                >  1:2414789/1‑78 (MQ=255)                                                         atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa                                <  2:2414789/78‑1 (MQ=255)                                                         atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAg                    >  2:2943988/1‑90 (MQ=255)                                                         atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAg                    >  2:346003/1‑90 (MQ=255)                                                           tAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTa                  <  1:309513/90‑1 (MQ=255)                                                           tAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTa                  <  1:1471671/90‑1 (MQ=255)                                                           tAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTa                  <  1:2043326/90‑1 (MQ=255)                                                               tATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTg              >  2:2061874/1‑90 (MQ=255)                                                               tATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTg              >  1:992056/1‑90 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATc                                         <  2:1429859/62‑1 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATc                                         >  1:1429859/1‑62 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg             <  2:2062888/90‑1 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg             <  2:1959143/90‑1 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg             >  1:471607/1‑90 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg             <  2:2952076/90‑1 (MQ=255)                                                                aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg             >  2:2975265/1‑90 (MQ=255)                                                                 tGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTGACTGgt            >  1:1677672/1‑90 (MQ=255)                                                                 tGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgt            >  1:187533/1‑90 (MQ=255)                                                                  ggCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtg           <  1:486842/90‑1 (MQ=255)                                                                  ggCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtg           <  1:616477/90‑1 (MQ=255)                                                                   gCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtgt          >  1:84323/1‑90 (MQ=255)                                                                       ccGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTg      >  2:1546603/1‑90 (MQ=255)                                                                           tGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACa  <  2:994635/90‑1 (MQ=255)                                                                              |                                                                                       CAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACA  >  NZ_CP009273/1980343‑1980506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑