Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,064,259	T→G	V93G (GTG→GGG)	BW25113_RS10440 →	GTPase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,064,259	0	T	G	100.0%	337.9 / NA	89	V93G (GTG→GGG)	BW25113_RS10440	GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (48/41);  total (48/41)

TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC  >  NZ_CP009273/2064172‑2064345                                                                                        |                                                                                       tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                      <  2:1358776/90‑1 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                      >  2:1977173/1‑86 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                      <  1:1977173/86‑1 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt                                                                                  >  1:2841968/1‑90 (MQ=255)     gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt                                                                                  >  1:1810920/1‑90 (MQ=255)     gtgGCTGATTAAAGCCGATGACCATGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt                                                                                  >  1:941055/1‑90 (MQ=255)      tgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGttt                                                                                 >  1:318039/1‑90 (MQ=255)       ggCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTtg                                                                                >  1:1648802/1‑90 (MQ=255)       ggCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTtg                                                                                >  1:2375784/1‑90 (MQ=255)         cTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCt                                                                                     >  2:143720/1‑83 (MQ=255)         cTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCt                                                                                     <  1:143720/83‑1 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc                                                                  >  2:999165/1‑90 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc                                                                  >  2:493771/1‑90 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc                                                                  <  2:908228/90‑1 (MQ=255)                     gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc                                                                  >  1:1461259/1‑90 (MQ=255)                            gTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                      >  1:1498579/1‑63 (MQ=255)                            gTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGc                                                                                      <  2:1498579/63‑1 (MQ=255)                            gTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACaaaa                                                           >  2:1297213/1‑90 (MQ=255)                            gTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACaaaa                                                           >  2:2232545/1‑90 (MQ=255)                                 ctgtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAg                                                      <  1:1697659/90‑1 (MQ=255)                                  tgtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGc                                                     >  2:1941439/1‑90 (MQ=255)                                   gtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGcc                                                    >  2:985824/1‑90 (MQ=255)                                   gtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGcc                                                    >  1:2552063/1‑90 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  2:1068527/90‑1 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  1:1591506/90‑1 (MQ=255)                                    tctgtGGATGAGTATTTCTGGCGACACATCCGGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  1:1941439/90‑1 (MQ=255)                                       gtgGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGc                                                >  2:2569552/1‑90 (MQ=255)                                       gtgGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGc                                                >  2:235582/1‑90 (MQ=255)                                         ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa                                              <  2:1338250/90‑1 (MQ=255)                                         ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa                                              <  2:1648802/90‑1 (MQ=255)                                         ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa                                              <  1:2232545/90‑1 (MQ=255)                                           aTGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATg                                            >  2:929821/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTgtgg                                                                             >  1:2107918/1‑55 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTgtgg                                                                             <  2:2107918/55‑1 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAGCGGAGCCCTGCCATGaa                                          >  1:1946549/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:2067583/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:1676949/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  1:293061/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  1:2765084/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:2578914/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  1:967516/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:3025696/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:1246698/1‑90 (MQ=255)                                             gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa                                          >  2:384862/1‑90 (MQ=255)                                              aGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                         <  1:490201/90‑1 (MQ=255)                                              aGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                         <  1:235582/90‑1 (MQ=255)                                              aGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                         <  2:1431861/90‑1 (MQ=255)                                                    tCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                         >  2:2184947/1‑84 (MQ=255)                                                    tCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt                                         <  1:2184947/84‑1 (MQ=255)                                                    tCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGata                                   >  1:1221350/1‑90 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                              <  2:1126986/90‑1 (MQ=255)                                                         cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc                              <  2:2317289/90‑1 (MQ=255)                                                          gACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCg                             <  1:134349/90‑1 (MQ=255)                                                          gACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCg                             <  2:319386/90‑1 (MQ=255)                                                          gACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCg                             <  1:384862/90‑1 (MQ=255)                                                           acacATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCgg                            >  2:2381250/1‑90 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  1:112676/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  2:941055/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  2:688665/90‑1 (MQ=255)                                                                     cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  1:2103882/90‑1 (MQ=255)                                                                       gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtc                >  2:2786783/1‑90 (MQ=255)                                                                       gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtc                <  1:2578914/90‑1 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  1:35225/54‑1 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   >  2:35225/1‑54 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt                                             >  2:443859/1‑60 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt                                             <  1:443859/60‑1 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  2:536361/87‑1 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  >  1:536361/1‑87 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtct               >  2:507628/1‑90 (MQ=255)                                                                        tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtct               >  2:370155/1‑90 (MQ=255)                                                                         gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  <  1:1953557/86‑1 (MQ=255)                                                                         gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt                  >  2:1953557/1‑86 (MQ=255)                                                                         gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc              >  1:909281/1‑90 (MQ=255)                                                                         gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc              >  2:2171075/1‑90 (MQ=255)                                                                         gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGGATTCAGCCTtctc              >  1:2329958/1‑90 (MQ=255)                                                                            gACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  2:426962/50‑1 (MQ=255)                                                                            gACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   >  1:426962/1‑50 (MQ=255)                                                                              cATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgca         >  2:2048250/1‑90 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac        <  2:2129934/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac        <  1:2067583/90‑1 (MQ=255)                                                                               aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac        <  1:507628/90‑1 (MQ=255)                                                                                tCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcaca       <  1:2569552/90‑1 (MQ=255)                                                                                 cagcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   >  1:1252020/1‑45 (MQ=255)                                                                                 cagcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc                                                   <  2:1252020/45‑1 (MQ=255)                                                                                   gcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggc    <  1:2825896/90‑1 (MQ=255)                                                                                   gcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggc    <  2:909281/90‑1 (MQ=255)                                                                                   gcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcaca       >  2:59060/1‑87 (MQ=255)                                                                                   gcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcaca       <  1:59060/87‑1 (MQ=255)                                                                                     agGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggcac  <  1:370155/90‑1 (MQ=255)                                                                                        |                                                                                       TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC  >  NZ_CP009273/2064172‑2064345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑