Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,131,004	A→G	intergenic (‑280/‑379)	wza ← / → yegH	polysaccharide export protein Wza/TerC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,131,004	0	A	G	100.0%	235.6 / NA	62	intergenic (‑280/‑379)	wza/yegH	polysaccharide export protein Wza/TerC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/35);  total (27/35)

TGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGG  >  NZ_CP009273/2130917‑2131088                                                                                        |                                                                                     tGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGgaa                                                                                    >  2:446618/1‑90 (MQ=255)      ttttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAAc                                                                               <  2:1528893/90‑1 (MQ=255)          aGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTcc                                                                           <  1:229884/90‑1 (MQ=255)          aGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTcc                                                                           <  1:1890776/90‑1 (MQ=255)           gCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCg                                                                          <  1:1570672/90‑1 (MQ=255)            cTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGa                                                                         >  1:2061985/1‑90 (MQ=255)             tACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGaa                                                                        >  2:2814762/1‑90 (MQ=255)                  aTACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATaa                                                                   <  1:1216783/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                <  2:2061985/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                <  2:2613282/90‑1 (MQ=255)                      acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                               <  1:1137962/90‑1 (MQ=255)                        tGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTc                                                                            >  2:466286/1‑75 (MQ=255)                        tGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTc                                                                            <  1:466286/75‑1 (MQ=255)                        tGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                               >  2:439457/1‑88 (MQ=255)                        tGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                               <  1:439457/88‑1 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGTCa                                                            >  2:1343928/1‑90 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  1:1699964/1‑90 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  2:2682488/1‑90 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  1:54031/1‑90 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                            >  1:2458824/1‑90 (MQ=255)                           tttAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACAtt                                                          <  1:446618/90‑1 (MQ=255)                            ttAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTa                                                         >  2:63587/1‑90 (MQ=255)                            ttAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTa                                                         >  1:758022/1‑90 (MQ=255)                              aGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATg                                                       >  1:1150511/1‑90 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAAtt                                                                                  <  1:462979/58‑1 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAAtt                                                                                  >  2:462979/1‑58 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACAtt                                                          >  2:1562069/1‑82 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACAtt                                                          <  1:1562069/82‑1 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAt                                                  >  1:1579957/1‑90 (MQ=255)                                    aTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGTCATTATGGTAAtt                                                 <  1:1343928/90‑1 (MQ=255)                                    aTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAtt                                                 <  1:136110/90‑1 (MQ=255)                                    aTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAtt                                                 <  2:2511403/90‑1 (MQ=255)                                              cccTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGc                                       >  1:462058/1‑90 (MQ=255)                                              cccTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGc                                       >  1:908755/1‑90 (MQ=255)                                                ctctCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                <  1:1306043/63‑1 (MQ=255)                                                ctctCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                >  2:1306043/1‑63 (MQ=255)                                                 tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCttt                                    >  2:2245314/1‑90 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaat                               <  1:1612009/90‑1 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaat                               <  1:282919/90‑1 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaat                               <  1:1866136/90‑1 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaat                               <  2:54031/90‑1 (MQ=255)                                                        aTGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaataa                             >  2:2418381/1‑90 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCg                                                                          <  2:923257/43‑1 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCg                                                                          >  1:923257/1‑43 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                >  2:2130977/1‑53 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                                <  1:2130977/53‑1 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                           >  2:1764981/1‑90 (MQ=255)                                                               cGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                               <  2:2172612/49‑1 (MQ=255)                                                               cGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                               >  1:2172612/1‑49 (MQ=255)                                                               cGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagga                      <  2:1747202/90‑1 (MQ=255)                                                               cGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagga                      <  1:1280558/90‑1 (MQ=255)                                                                    atgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg                 <  2:758022/90‑1 (MQ=255)                                                                       agaAAGGGTTATTACGGGAATTAACTTCCGAATATAAgg                                                                 >  2:2531148/1‑39 (MQ=39)                                                                       agaAAGGGTTATTACGGGAATTAACTTCCGAATATAAgg                                                                 <  1:2531148/39‑1 (MQ=39)                                                                             ggTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGc        >  2:1464855/1‑90 (MQ=255)                                                                               ttattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa      <  2:1805338/90‑1 (MQ=255)                                                                               ttattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa      <  2:1699964/90‑1 (MQ=255)                                                                               ttattaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa      <  1:1817219/90‑1 (MQ=255)                                                                                 attaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAAc    <  2:1380685/90‑1 (MQ=255)                                                                                 attaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAAc    <  2:1864863/90‑1 (MQ=255)                                                                                  ttaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAggagga                   <  2:123206/74‑1 (MQ=255)                                                                                  ttaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAggagga                   >  1:123206/1‑74 (MQ=255)                                                                                   taCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACgg  <  2:2193123/90‑1 (MQ=255)                                                                                        |                                                                                     TGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGG  >  NZ_CP009273/2130917‑2131088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑