BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,345,414	T→G	H147P (CAC→CCC)	glpT ←	glycerol‑3‑phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,345,414	0	T	G	100.0%	457.5 / NA	119	H147P (CAC→CCC)	glpT	glycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (58/61); total (58/61)

Only 100 of 119 total aligned reads displayed.

AACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTC  >  NZ_CP009273/2345327‑2345500

aaCAGCAGCGGCGGAATACCCCCCCCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:2734535/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:3017361/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:1224466/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:278417/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  2:434057/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:1026006/90‑1 (MQ=255)

aaCAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  2:1225845/90‑1 (MQ=255)

   agcagcGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  2:762090/90‑1 (MQ=255)

   agcagcGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  1:2009140/90‑1 (MQ=255)

      agcGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATa                                                                                <  2:2565099/90‑1 (MQ=255)

        cggcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGt                                                                              >  1:1300702/1‑90 (MQ=255)

         ggcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTa                                                                             >  2:2245228/1‑90 (MQ=255)

         ggcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTa                                                                             >  2:1370358/1‑90 (MQ=255)

              aaTACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGAcc                                                                        <  1:885623/90‑1 (MQ=255)

                    acctccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcgg                                                                  >  2:3014798/5‑90 (MQ=255)

                    accaccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcgg                                                                  >  2:2707994/1‑90 (MQ=255)

                       accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTAc                                                                            >  1:2875079/1‑77 (MQ=255)

                       accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTAc                                                                            <  2:2875079/77‑1 (MQ=255)

                       accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggcgg                                                               >  1:633270/1‑90 (MQ=255)

                       accGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggcgg                                                               >  2:1504601/1‑90 (MQ=255)

                        ccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACggcggc                                                              <  2:2491386/90‑1 (MQ=255)

                             gTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   >  1:196745/1‑64 (MQ=255)

                             gTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  2:196745/64‑1 (MQ=255)

                               tgtgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTAc                                                                            <  2:2976096/69‑1 (MQ=255)

                                gtgCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCAt                                                      <  1:360345/90‑1 (MQ=255)

                                  gcgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   >  2:1422976/1‑59 (MQ=255)

                                  gcgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  1:1422976/59‑1 (MQ=255)

                                  gcgcACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGcc                                                             >  1:148574/1‑81 (MQ=255)

                                       caGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTgg                                               <  2:1780711/90‑1 (MQ=255)

                                         gTTCCACACTGACTCAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaa                                             >  2:604588/1‑90 (MQ=255)

                                         gTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      >  2:622203/1‑49 (MQ=255)

                                         gTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  1:622203/49‑1 (MQ=255)

                                          ttCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaac                                            >  2:1440588/1‑90 (MQ=255)

                                          ttCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaac                                            >  2:94394/1‑90 (MQ=255)

                                            ccACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaacca                                          <  1:1440588/90‑1 (MQ=255)

                                                 cTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGc                                     >  2:2651825/1‑90 (MQ=255)

                                                 cTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGc                                     >  1:2933448/1‑90 (MQ=255)

                                                 cTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGc                                     >  1:2101973/1‑90 (MQ=255)

                                                  tGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCCCCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    <  1:2154623/90‑1 (MQ=255)

                                                  tGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    <  2:1092379/90‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGc                                                                                      <  2:1130596/39‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   >  2:180490/1‑42 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  1:180490/42‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   >  2:86262/1‑42 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCAcc                                                                                   <  1:86262/42‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGt                                                                              >  1:634401/1‑47 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGt                                                                              <  2:634401/47‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggc                                                                 <  2:2415241/60‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACAcggc                                                                 >  1:2415241/1‑60 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCa                                                       >  1:305771/1‑70 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaacca                                          <  2:421278/83‑1 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGaacca                                          >  1:421278/1‑83 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:594567/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:350005/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:2398603/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:1246439/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:1315911/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:2177322/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:1377933/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:1962511/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:2242961/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:1459673/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:398976/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  1:2492483/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:2924884/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                                   >  2:718219/1‑90 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    >  2:1915011/1‑89 (MQ=255)

                                                   gACACAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    <  1:1915011/89‑1 (MQ=255)

                                                    acacAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCaga                                  <  2:1232662/90‑1 (MQ=255)

                                                    acacAATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCaga                                  <  1:96915/90‑1 (MQ=255)

                                                       caATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGa                               <  2:108374/90‑1 (MQ=255)

                                                       caATGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGa                               <  1:765931/90‑1 (MQ=255)

                                                         aTGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaac                             >  1:1038151/1‑90 (MQ=255)

                                                         aTGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaac                             <  2:594567/90‑1 (MQ=255)

                                                         aTGCCGCCACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaac                             >  2:2956969/1‑90 (MQ=255)

                                                            ccgccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          <  1:659470/90‑1 (MQ=255)

                                                            ccgccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          <  1:2398603/90‑1 (MQ=255)

                                                            ccgccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          <  2:2242961/90‑1 (MQ=255)

                                                            ccgccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          <  2:214431/90‑1 (MQ=255)

                                                             cgccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAacaaca                         >  1:2362883/1‑90 (MQ=255)

                                                              gccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAg                        <  2:2933448/90‑1 (MQ=255)

                                                              gccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAg                        >  2:407272/1‑90 (MQ=255)

                                                              gccACGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAg                        >  2:432075/1‑90 (MQ=255)

                                                                cACGTTCTTTCTGCGCCCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTa                      <  1:1807635/90‑1 (MQ=255)

                                                                 aCGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    >  2:2515144/1‑75 (MQ=255)

                                                                 aCGTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCa                                    <  1:2515144/75‑1 (MQ=255)

                                                                   gTTCTTTCTGCGACCACCAGGGCCCCATAGTACGACCACACGGCGGCCCCCCCACCCCCTGGAACCAACCGCAGAGGAACAACAGTACaa                   <  1:2245228/90‑1 (MQ=255)

                                                                   gTTCTTTCTGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACaa                   <  1:894372/90‑1 (MQ=255)

                                                                          ctGCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCAc            <  1:2924884/90‑1 (MQ=255)

                                                                           tGCGACCACCAGGGCACCATAGTACGACCACACGGCGGcc                                                             >  2:2660459/1‑40 (MQ=255)

                                                                           tGCGACCACCAGGGCACCATAGTACGACCACACGGCGGcc                                                             <  1:2660459/40‑1 (MQ=255)

                                                                            gCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          >  1:2239945/1‑74 (MQ=255)

                                                                            gCGACCACCAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                          <  2:2239945/74‑1 (MQ=255)

                                                                             cGACCACCAGGGCACCATAGTACGACCACACGGCGGcc                                                             <  1:685635/38‑1 (MQ=39)

                                                                             cGACCACCAGGGCACCATAGTACGACCACACGGCGGcc                                                             >  2:685635/1‑38 (MQ=39)

                                                                              gACCACCAGGGCACCATAGTACGACCACACGGCGGCCAccc                                                         <  2:2715956/41‑1 (MQ=255)

                                                                               accaccAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa       <  2:2492483/90‑1 (MQ=255)

                                                                               accaccAGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCaa       <  1:946488/90‑1 (MQ=255)

                                                                                ccaccaGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAt      >  1:905490/1‑90 (MQ=255)

                                                                                    caGGGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTc  <  2:913/90‑1 (MQ=255)

AACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTC  >  NZ_CP009273/2345327‑2345500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`