Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,396,099	G→C	A413G (GCC→GGC)	nuoC ←	NADH‑quinone oxidoreductase subunit C/D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,396,099	0	G	C	100.0%	242.6 / NA	64	A413G (GCC→GGC)	nuoC	NADH‑quinone oxidoreductase subunit C/D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (37/27);  total (37/27)

GCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCA  >  NZ_CP009273/2396013‑2396181                                                                                       |                                                                                   gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  1:797908/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  1:698371/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  2:466244/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  2:2704865/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  2:1324925/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  2:393889/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  2:1421873/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  1:1978806/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  1:2729201/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                                 >  1:1965662/1‑90 (MQ=255)   gcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCa                                                                               <  2:1333778/90‑1 (MQ=255)    ccTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCaa                                                                              <  1:2552095/90‑1 (MQ=255)    ccTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCaa                                                                              <  1:702758/90‑1 (MQ=255)     cTTCCGCACGTCGAAGTCGATCCCGGTAGCCCGCCGGCCCGCGCCCGTGGTGCCCAACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                                             <  1:1875162/90‑1 (MQ=255)     cTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                                             <  2:2840803/90‑1 (MQ=255)     cTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                                             <  1:750460/90‑1 (MQ=255)     cTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                                             <  1:2567926/90‑1 (MQ=255)            aCGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTgg                                                                      >  2:455574/1‑90 (MQ=255)            aCGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTgg                                                                      >  2:2079913/1‑90 (MQ=255)            aCGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTgg                                                                      >  1:2607881/1‑90 (MQ=255)            aCGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTgg                                                                      >  1:2475204/1‑90 (MQ=255)              gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGa                                                                    >  1:1050886/1‑90 (MQ=255)              gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGa                                                                    >  1:1952309/1‑90 (MQ=255)              gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGa                                                                    >  1:1866425/1‑90 (MQ=255)                cGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAAc                                                                  <  1:475715/90‑1 (MQ=255)                     tCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCt                                                             >  2:77765/1‑90 (MQ=255)                       gATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCttt                                                           >  2:413974/1‑90 (MQ=255)                       gATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCttt                                                           >  1:448382/1‑90 (MQ=255)                       gATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCttt                                                           >  2:1164750/1‑90 (MQ=255)                       gATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCttt                                                           >  2:788593/1‑90 (MQ=255)                       gATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCttt                                                           >  1:989955/1‑90 (MQ=255)                        aTCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                                          <  1:2406320/90‑1 (MQ=255)                        aTCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                                          <  1:393889/90‑1 (MQ=255)                        aTCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                                          <  1:1421873/90‑1 (MQ=255)                           ccGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGa                                                       >  2:873764/1‑90 (MQ=255)                             ggTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     <  2:2475204/90‑1 (MQ=255)                             ggTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     <  2:978963/90‑1 (MQ=255)                               tAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATgg                                                   <  2:1533050/90‑1 (MQ=255)                                      cAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGccc                                                                         <  1:1979000/61‑1 (MQ=255)                                      cAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGccc                                                                         >  2:1979000/1‑61 (MQ=255)                                      cAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTg                                            >  1:2205336/1‑90 (MQ=255)                                       aGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGc                                           >  1:858984/1‑90 (MQ=255)                                        ggCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCaa                                                                              <  1:1979928/54‑1 (MQ=255)                                        ggCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCaa                                                                              >  2:1979928/1‑54 (MQ=255)                                            cgcgCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAgcgc                                      <  1:1164750/90‑1 (MQ=255)                                              cgcCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCGACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGcgccg                                    >  1:1230191/1‑90 (MQ=255)                                                 cAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCtt                                 <  1:873764/90‑1 (MQ=255)                                                  aGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCttt                                >  1:1003752/1‑90 (MQ=255)                                                  aGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCttt                                >  2:1605770/1‑90 (MQ=255)                                                  aGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCttt                                >  1:951072/1‑90 (MQ=255)                                                  aGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCttt                                >  2:257305/1‑90 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCa                                                                               >  1:1229845/1‑37 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCa                                                                               <  2:1229845/37‑1 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATgg                                                   >  2:1367940/1‑65 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATgg                                                   <  1:1367940/65‑1 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTa                          >  1:2958263/1‑90 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTa                          >  1:1600787/1‑90 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTa                          >  2:2272329/1‑90 (MQ=255)                                                        gCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTa                          >  2:1655755/1‑90 (MQ=255)                                                          cccACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     <  1:324312/61‑1 (MQ=255)                                                          cccACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     >  2:324312/1‑61 (MQ=255)                                                             aCTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     >  1:2709919/1‑58 (MQ=255)                                                             aCTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                                     <  2:2709919/58‑1 (MQ=255)                                                             aCTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACg                     >  2:1794785/1‑90 (MQ=255)                                                             aCTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACg                     >  1:1152074/1‑90 (MQ=255)                                                                 cAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAg                 <  2:488866/90‑1 (MQ=255)                                                                  aGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGa                >  1:1697828/1‑90 (MQ=255)                                                                  aGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGa                >  1:623798/1‑90 (MQ=255)                                                                     gcCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGt             <  2:1697828/90‑1 (MQ=255)                                                                       ctctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGttt           >  2:1354131/1‑90 (MQ=255)                                                                        tctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTc          >  2:513011/1‑90 (MQ=255)                                                                             cgcgCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGTAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCAt     <  2:1050886/90‑1 (MQ=255)                                                                             cgcgCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCAt     <  1:413974/90‑1 (MQ=255)                                                                                gcCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCa  <  1:788593/90‑1 (MQ=255)                                                                                       |                                                                                   GCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCA  >  NZ_CP009273/2396013‑2396181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑