Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,670,359	T→C	G454G (GGA→GGG)	yphE ←	sugar ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,670,359	0	T	C	100.0%	278.0 / NA	73	G454G (GGA→GGG)	yphE	sugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/28);  total (45/28)

GAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAA  >  NZ_CP009273/2670273‑2670445                                                                                       |                                                                                       gagaACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCt                                                                                     >  1:701067/1‑90 (MQ=255)           cgtgcTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                           <  2:701067/90‑1 (MQ=255)           cgtgcTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                           <  2:479446/90‑1 (MQ=255)               cTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTc                                                                       <  2:1822673/90‑1 (MQ=255)                tGTAATAACAGGATGTGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                      <  2:1125069/90‑1 (MQ=255)                tGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                      <  2:661017/90‑1 (MQ=255)                tGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                      <  1:808715/90‑1 (MQ=255)                   aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                                   <  2:1814759/90‑1 (MQ=255)                      aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagc                                                                             >  2:2363375/1‑77 (MQ=255)                      aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagc                                                                             <  1:2363375/77‑1 (MQ=255)                      aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACa                                                                >  2:2159064/1‑90 (MQ=255)                      aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACa                                                                >  2:23716/1‑90 (MQ=255)                      aaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACa                                                                >  1:1501067/1‑90 (MQ=255)                        cAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAAt                                                              >  2:2530006/1‑90 (MQ=255)                        cAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAAt                                                              <  2:1501067/90‑1 (MQ=255)                         aGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                           >  2:257975/1‑76 (MQ=255)                         aGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                           <  1:257975/76‑1 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           <  1:673594/90‑1 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  2:2427739/1‑90 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  2:2205834/1‑90 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  2:2715961/1‑90 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  2:570030/1‑90 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           <  2:700155/90‑1 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  2:856526/1‑90 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCAAGCTCACGGACAATACg                                                           <  2:1712805/90‑1 (MQ=255)                           gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAACCAGCTCACGGACAATACg                                                           >  2:284175/1‑90 (MQ=255)                             tGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGt                                                         <  2:1310910/90‑1 (MQ=255)                                ggTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTaaa                                                      <  1:1413186/90‑1 (MQ=255)                                         ccAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGtt                                             >  1:265573/1‑90 (MQ=255)                                           aGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTg                                           >  2:435055/1‑90 (MQ=255)                                           aGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTg                                           >  1:1848677/1‑90 (MQ=255)                                               gCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                       <  2:762024/90‑1 (MQ=255)                                               gCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                       >  2:2233465/1‑90 (MQ=255)                                               gCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                       >  1:424761/1‑90 (MQ=255)                                                 aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                      >  1:1644226/1‑57 (MQ=255)                                                 aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                      <  2:1644226/57‑1 (MQ=255)                                                 aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCg                                     >  2:72900/1‑90 (MQ=255)                                                 aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCg                                     <  1:2604931/90‑1 (MQ=255)                                                 aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCg                                     >  1:27123/1‑90 (MQ=255)                                                       tccACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           <  2:2553013/62‑1 (MQ=255)                                                       tccACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                           >  1:2553013/1‑62 (MQ=255)                                                       tccACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCg                               >  1:1149117/1‑90 (MQ=255)                                                            ttCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgcc                          <  1:302499/90‑1 (MQ=255)                                                            ttCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgcc                          <  1:2434795/90‑1 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  1:336046/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  1:1109519/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         <  1:856526/90‑1 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  1:2629318/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  2:1021084/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  2:330035/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  1:474866/1‑90 (MQ=255)                                                             tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                         >  2:1097336/1‑90 (MQ=255)                                                               aCTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcg                       >  2:519367/1‑90 (MQ=255)                                                               aCTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcg                       >  2:1237865/1‑90 (MQ=255)                                                                cTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcgc                      >  1:2841988/1‑90 (MQ=255)                                                                    agaTAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTg                  <  1:435055/90‑1 (MQ=255)                                                                     gaTAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTgg                 >  2:2732046/1‑90 (MQ=255)                                                                     gaTAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTgg                 >  2:2969958/1‑90 (MQ=255)                                                                       tAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCt               >  2:1216294/1‑90 (MQ=255)                                                                       tAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCt               >  2:687782/1‑90 (MQ=255)                                                                         aaCACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcg             >  2:2302783/1‑90 (MQ=255)                                                                            accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcgtcg          >  2:1708543/1‑90 (MQ=255)                                                                            accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcgtcg          >  1:871511/1‑90 (MQ=255)                                                                             ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGt                                                         <  2:2535583/42‑1 (MQ=255)                                                                             ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGt                                                         >  1:2535583/1‑42 (MQ=255)                                                                             ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                       >  1:675967/1‑60 (MQ=255)                                                                             ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCtt                                       <  2:675967/60‑1 (MQ=255)                                                                              cacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAg        <  1:128140/90‑1 (MQ=255)                                                                              cacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAg        <  2:809278/90‑1 (MQ=255)                                                                               acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTa                                                        >  1:1766442/1‑41 (MQ=255)                                                                               acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTa                                                        <  2:1766442/41‑1 (MQ=255)                                                                               acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc       >  1:162425/1‑90 (MQ=255)                                                                               acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc       >  1:917263/1‑90 (MQ=255)                                                                                    tttcCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaacaa  >  1:1752405/1‑90 (MQ=255)                                                                                       |                                                                                       GAGAACGTGCCGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAA  >  NZ_CP009273/2670273‑2670445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑