Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,467	T→C	I193V (ATT→GTT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,467	0	T	C	100.0%	371.4 / NA	97	I193V (ATT→GTT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (65/32);  total (65/32)

TTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGA  >  NZ_CP009273/3786384‑3786553                                                                                    |                                                                                       ttCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGaa                                                                                  <  1:3010950/90‑1 (MQ=255)           aaTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAAcc                                                                        >  1:1534553/1‑90 (MQ=255)              ttATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCtgt                                                                     <  2:2640051/90‑1 (MQ=255)              ttATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCtgt                                                                     <  2:1452752/90‑1 (MQ=255)                ataGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCtgttg                                                                   >  2:53044/1‑90 (MQ=255)                  aGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTgttgtt                                                                 <  2:2572213/90‑1 (MQ=255)                  aGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTgttgtt                                                                 <  2:550561/90‑1 (MQ=255)                  aGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTgttgtt                                                                 <  1:1619379/90‑1 (MQ=255)                  aGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCACCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTgttgtt                                                                 <  1:248581/90‑1 (MQ=255)                    aaTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTga                                                               <  2:256645/90‑1 (MQ=255)                     aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  2:788595/1‑90 (MQ=255)                     aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  1:87006/1‑90 (MQ=255)                     aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  2:1215025/1‑90 (MQ=255)                     aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  2:1394921/1‑90 (MQ=255)                     aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  2:1560792/1‑90 (MQ=255)                      ttCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgaga                                                             <  2:14435/90‑1 (MQ=255)                        cTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtg                                                           >  2:2265654/1‑90 (MQ=255)                           tCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                        <  1:1215025/90‑1 (MQ=255)                           tCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                        <  1:1394921/90‑1 (MQ=255)                               tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                        >  2:2918582/1‑86 (MQ=255)                               tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                        <  1:2918582/86‑1 (MQ=255)                               tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGt                                                    <  2:1338374/90‑1 (MQ=255)                                  aTGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAg                                                 >  1:510128/1‑90 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               >  2:818217/1‑90 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               <  2:1158285/90‑1 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               <  1:1390903/90‑1 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               <  1:1584981/90‑1 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               <  1:2242719/90‑1 (MQ=255)                                    gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               >  2:2486809/1‑90 (MQ=255)                                      tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCt                                             >  2:1709742/1‑90 (MQ=255)                                      tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCt                                             <  2:2175721/90‑1 (MQ=255)                                      tgtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCt                                             <  1:53044/90‑1 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGc                                                >  2:1586896/1‑86 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGc                                                <  1:1586896/86‑1 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                            >  2:418606/1‑90 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                            >  2:2998730/1‑90 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                            >  1:2516732/1‑90 (MQ=255)                                       gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                            >  2:1029170/1‑90 (MQ=255)                                        tgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCttt                                           >  1:2349980/1‑90 (MQ=255)                                              aacaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTtat                                     >  2:432728/1‑90 (MQ=255)                                              aacaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTtat                                     >  2:317003/1‑90 (MQ=255)                                               acaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCt                                                                       >  2:2983739/1‑55 (MQ=255)                                               acaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCt                                                                       <  1:2983739/55‑1 (MQ=255)                                                   ttGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGa                                >  2:1624689/1‑90 (MQ=255)                                                   ttGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGa                                >  2:1303108/1‑90 (MQ=255)                                                     gcgcCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGaaa                              >  1:1288219/1‑90 (MQ=255)                                                         ccccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGAt                          <  1:594805/90‑1 (MQ=255)                                                          cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTga                                                               <  2:49668/52‑1 (MQ=255)                                                          cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTga                                                               >  1:49668/1‑52 (MQ=255)                                                          cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                         <  2:2788360/90‑1 (MQ=255)                                                           ccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGc                        <  2:540076/90‑1 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               >  2:711304/1‑58 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                               <  1:711304/58‑1 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  2:1184987/1‑90 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  1:176644/1‑90 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  1:2801712/1‑90 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  1:2255967/1‑90 (MQ=255)                                                                    tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  2:1557257/1‑90 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              >  2:649737/1‑41 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                              <  1:649737/41‑1 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGa                           >  2:356504/1‑76 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGa                           <  1:356504/76‑1 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc             >  1:3009107/1‑90 (MQ=255)                                                                      taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc             >  1:791557/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATTAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  2:676035/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  2:2438137/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  2:886852/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  2:1928522/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  1:1404634/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  1:2305178/1‑90 (MQ=255)                                                                        cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat           >  1:1091564/1‑90 (MQ=255)                                                                         aTGATTGCCAACCATGAAATTAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatc          <  1:676035/90‑1 (MQ=255)                                                                         aTGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCC‑TTTTTTATAGTGAAAAGATGCATCTTACTTtcatca         >  2:2211947/1‑90 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGccc                                              <  2:2843839/53‑1 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGccc                                              >  1:2843839/1‑53 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca         >  1:1150951/1‑90 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca         >  1:1548869/1‑90 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca         >  1:2037403/1‑90 (MQ=255)                                                                          tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca         >  2:1043947/1‑90 (MQ=255)                                                                           gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa        >  1:1997705/1‑90 (MQ=255)                                                                           gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa        >  2:635653/1‑90 (MQ=255)                                                                           gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTACAGTGAAAAGATGCATCTTACTTTCATCaa        >  2:2021956/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAGAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  1:767912/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  1:2814503/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  1:1771029/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  1:1639709/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  2:1198728/1‑90 (MQ=255)                                                                             ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt      >  2:1693285/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               >  2:943554/1‑79 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt               <  1:943554/79‑1 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:405311/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:694449/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:2454178/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:1201405/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:1137007/1‑90 (MQ=255)                                                                               gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg    >  2:893115/1‑90 (MQ=255)                                                                                 cAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGa  <  2:2294738/90‑1 (MQ=255)                                                                                    |                                                                                       TTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGA  >  NZ_CP009273/3786384‑3786553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑