Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,903 T→A D27E (GAT→GAA)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9030TA100.0% 383.5 / NA 104D27E (GAT→GAA) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (52/52);  total (52/52)

Only 100 of 104 total aligned reads displayed.

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA  >  NZ_CP009273/49820‑49987
                                                                                   |                                                                                    
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATTGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                <  1:1527/90‑1 (MQ=255)
tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                <  1:1018236/90‑1 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                               >  1:1343067/1‑90 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                               <  1:1755795/90‑1 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                               <  1:187769/90‑1 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                               <  2:1425447/90‑1 (MQ=255)
 cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCt                                                                               <  1:2306880/90‑1 (MQ=255)
     gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGtt                                                                           <  2:1343067/90‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGGAAACCCCACGCCGTGGAACCTGCCTGCCGAACTCGCCTGGATTaa                                                                        >  1:2882047/1‑90 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                <  2:1635102/82‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                <  2:2931240/82‑1 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                >  1:1635102/1‑82 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                >  1:2931240/1‑82 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa                                                                        >  1:413914/1‑90 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa                                                                        >  2:1431065/1‑90 (MQ=255)
        cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa                                                                        >  1:17006/1‑90 (MQ=255)
         aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                                       >  2:2487429/1‑90 (MQ=255)
         aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                                       >  1:255499/1‑90 (MQ=255)
          gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAAc                                                                      >  2:2534458/1‑90 (MQ=255)
          gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAAc                                                                      <  1:2939974/90‑1 (MQ=255)
          gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAAc                                                                      >  2:1374328/1‑90 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  2:1542361/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  1:1374328/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  2:2682371/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  2:17006/90‑1 (MQ=255)
              gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  1:2534458/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 <  1:1854026/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 <  1:320613/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 <  1:2597709/90‑1 (MQ=255)
               aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 >  2:802631/1‑90 (MQ=255)
                  gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACAcc                                                              >  1:932555/1‑90 (MQ=255)
                  gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACAcc                                                              >  2:2196564/1‑90 (MQ=255)
                  gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACAcc                                                              >  2:2590665/1‑90 (MQ=255)
                   cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             <  2:2013395/90‑1 (MQ=255)
                   cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             <  2:1240753/90‑1 (MQ=255)
                   cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             <  2:512215/90‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  >  1:1281300/1‑84 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  1:978030/84‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  2:1281300/84‑1 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                            >  2:328836/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                            >  1:2901780/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                            >  2:2271017/1‑90 (MQ=255)
                    ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                            >  2:1237002/1‑90 (MQ=255)
                        ttAGCGGTAGATCGCGTTATCGGCATTGGAAACGCCATGCCGGGGAACCTGCCTGCCGGACTCGCCTGGGGTAAACGCAACACCTtaaaa                                                        >  1:1728285/1‑89 (MQ=255)
                         tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaata                                                       <  1:174603/90‑1 (MQ=255)
                         tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaata                                                       <  1:2542596/90‑1 (MQ=255)
                         tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTCCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaata                                                       <  1:2196564/90‑1 (MQ=255)
                          aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaataa                                                      <  1:663969/90‑1 (MQ=255)
                            cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                    <  2:2514827/90‑1 (MQ=255)
                              gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                                  <  1:1437789/90‑1 (MQ=255)
                                aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                <  2:2901780/90‑1 (MQ=255)
                                aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                                <  1:587965/90‑1 (MQ=255)
                                 gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  >  2:899476/1‑71 (MQ=255)
                                 gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                                  <  1:899476/71‑1 (MQ=255)
                                   tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGGACCGGCCTGGCGAACTCGCCTGGTTTAAACGCAACACCCTAAATAAACCCGTggt                                             >  1:2026588/1‑88 (MQ=255)
                                    cgcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAtt                                            >  1:1171900/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAaca                                                                >  1:114778/1‑69 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAaca                                                                <  2:114778/69‑1 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                           >  2:625483/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                           >  2:1657096/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                           >  2:204012/1‑90 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGAACTCGCCTGGTTTAAACGCAac                                                                 <  1:1436924/68‑1 (MQ=255)
                                     gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGAACTCGCCTGGTTTAAACGCAac                                                                 >  2:1436924/1‑68 (MQ=255)
                                        ttATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                        <  2:2519414/90‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                <  2:48335/50‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc                                                                                >  1:48335/1‑50 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACAcc                                                              >  1:2330518/1‑68 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACAcc                                                              <  2:2330518/68‑1 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                        >  1:2519414/1‑90 (MQ=255)
                                        ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                        >  1:887013/1‑90 (MQ=255)
                                         tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                       <  2:255499/90‑1 (MQ=255)
                                          aTCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                      <  1:10041/90‑1 (MQ=255)
                                            cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                                    >  2:2265534/1‑90 (MQ=255)
                                              gCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc                                  <  1:575660/90‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTg                                                                              >  2:1192572/1‑44 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTg                                                                              <  1:1192572/44‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             >  2:611381/1‑61 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             <  1:611381/61‑1 (MQ=255)
                                                aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                <  1:1657096/90‑1 (MQ=255)
                                                    aaacCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                            <  2:2616410/90‑1 (MQ=255)
                                                    aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                            <  1:92203/90‑1 (MQ=255)
                                                    aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                            <  1:99907/90‑1 (MQ=255)
                                                    aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                            >  1:1159254/1‑90 (MQ=255)
                                                    aaaaCGCCATGCCGTGGAACCCGCCTGCCGAACTCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                            <  1:2897707/90‑1 (MQ=255)
                                                        cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                        >  1:3020639/1‑90 (MQ=255)
                                                            aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 <  2:1167876/45‑1 (MQ=255)
                                                            aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                                 >  1:1167876/1‑45 (MQ=255)
                                                            aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                    >  1:2879510/1‑90 (MQ=255)
                                                            aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                    >  2:2140592/1‑90 (MQ=255)
                                                            aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                    >  1:519196/1‑90 (MQ=255)
                                                             tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             >  1:2080817/1‑48 (MQ=255)
                                                             tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                             <  2:2080817/48‑1 (MQ=255)
                                                             tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                   >  2:1067722/1‑90 (MQ=255)
                                                             tGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                   >  1:1959440/1‑90 (MQ=255)
                                                                 gTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg               >  1:1087591/1‑90 (MQ=255)
                                                                         tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc         <  2:2232997/88‑1 (MQ=255)
                                                                         tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc         >  1:2232997/1‑88 (MQ=255)
                                                                         tgcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt       >  2:675705/1‑90 (MQ=255)
                                                                              gccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa  >  1:2988641/1‑90 (MQ=255)
                                                                              gccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa  >  2:2332912/1‑90 (MQ=255)
                                                                                   |                                                                                    
TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCA  >  NZ_CP009273/49820‑49987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑