Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,084,653	A→G	G358G (GGT→GGC)	frvB ←	PTS fructose‑like transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,084,653	0	A	G	100.0%	323.2 / NA	98	G358G (GGT→GGC)	frvB	PTS fructose‑like transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/43);  total (55/43)

TGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGA  >  NZ_CP009273/4084566‑4084738                                                                                        |                                                                                      tGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGcc                                                                                     <  1:321485/90‑1 (MQ=255)    gACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCccgc                                                                                  >  1:650223/1‑90 (MQ=255)    gACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCccgc                                                                                  >  2:793195/1‑90 (MQ=255)    gACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCccgc                                                                                  >  2:1930508/1‑90 (MQ=255)     aCTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCccgcc                                                                                 <  1:688738/90‑1 (MQ=255)       tgtgGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCcgccgc                                                                               >  1:1917469/1‑90 (MQ=255)        gtgGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCcgcg                                                                              >  2:349383/1‑90 (MQ=255)        gtgGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCcgcg                                                                              >  2:2847144/1‑90 (MQ=255)         tgGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGa                                                                             >  1:808334/1‑90 (MQ=255)          ggCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  1:236536/90‑1 (MQ=255)          ggCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  1:1903147/90‑1 (MQ=255)          ggCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  2:1045704/90‑1 (MQ=255)          ggCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  1:793195/90‑1 (MQ=255)          ggCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  2:2582707/90‑1 (MQ=255)          ggCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  1:1227343/90‑1 (MQ=255)            cACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACgg                                                                          <  2:3000575/90‑1 (MQ=255)              ccGACCACAATGGCACTACTGGCAGCCTCTTTTCCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggc                                                                        <  1:2055046/90‑1 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  1:162867/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:2504743/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:2438906/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  1:1472382/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:734554/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  1:2235097/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:484048/1‑90 (MQ=255)                gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAACGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                      >  2:2699590/1‑90 (MQ=255)                  ccACAATGGCACTCCTGGCAGCCTCTTTTTCCTGTGGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCa                                                                    <  1:3014550/90‑1 (MQ=255)                  ccACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCa                                                                    <  2:2320026/90‑1 (MQ=255)                  ccACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCa                                                                    >  2:1698110/1‑90 (MQ=255)                    acaATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATc                                                                  >  1:1899066/1‑90 (MQ=255)                    acaATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATc                                                                  <  1:2046739/90‑1 (MQ=255)                     caATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            <  1:1047874/79‑1 (MQ=255)                     caATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGAc                                                                            >  2:1047874/1‑79 (MQ=255)                     caATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCa                                                                 >  2:2227541/1‑90 (MQ=255)                       aTGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAgc                                                               <  2:2531512/90‑1 (MQ=255)                          gCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGcgcg                                                            >  1:2609287/1‑90 (MQ=255)                          gCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGcgcg                                                            >  1:778939/1‑90 (MQ=255)                              tactGGCAGCCTCTTTTTCCTGTGGGGAAAACAGTTTGGGCGCAGTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACaa                                                        <  2:1902575/90‑1 (MQ=255)                              tactGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggc                                                                        <  1:2621835/74‑1 (MQ=255)                              tactGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggc                                                                        >  2:2621835/1‑74 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:125944/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  1:970216/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:1641114/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  2:7272/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  1:1139030/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  1:1452862/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  1:1728466/1‑90 (MQ=255)                               actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                       >  1:2442120/1‑90 (MQ=255)                                  ggCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGcc                                                    >  1:2709391/1‑90 (MQ=255)                                  ggCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGcc                                                    >  1:1828516/1‑90 (MQ=255)                                  ggCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGcc                                                    >  1:1982619/1‑90 (MQ=255)                                    cAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcga                                                  >  1:2611026/1‑90 (MQ=255)                                    cAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcga                                                  >  1:356683/1‑90 (MQ=255)                                     acccTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                                 <  2:2678591/88‑1 (MQ=255)                                     aGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                                 <  2:2782041/90‑1 (MQ=255)                                     aGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                                 >  1:1819358/1‑90 (MQ=255)                                       ccTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgacga                                               >  2:674152/1‑90 (MQ=255)                                       ccTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgacga                                               <  2:562398/90‑1 (MQ=255)                                        ctttttttCCTGGCGGGTAAACAGTTTGGGGGCAATGAACGTCGAAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                              <  1:1329165/87‑1 (MQ=255)                                             tttCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGt                                         >  2:1491810/1‑90 (MQ=255)                                               tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                       <  2:970216/90‑1 (MQ=255)                                               tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                       <  1:497651/90‑1 (MQ=255)                                               tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                       <  1:7272/90‑1 (MQ=255)                                                ccTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAcc                                      <  1:1491810/90‑1 (MQ=255)                                                 cTGTCGGGTAACAAGTTTGGGAGCAAGGAACGTCGCAAGGCCCGCCGCGACGGGCGGCTTCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  2:1326845/90‑1 (MQ=255)                                                 cTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  2:2913185/90‑1 (MQ=255)                                                 cTGTCGGGTAAACAGTTTGGGCGCAAGGAACGTCCCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  1:2085983/90‑1 (MQ=255)                                                 cTGTCGGGTAAACAGTTTGGGCGCAACGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  1:2699590/90‑1 (MQ=255)                                                  tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     >  1:372493/1‑89 (MQ=255)                                                  tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                     <  2:372493/89‑1 (MQ=255)                                                    tCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                  >  2:2128570/1‑90 (MQ=255)                                                    tCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                  >  1:381701/1‑90 (MQ=255)                                                    tCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                                  >  2:950140/1‑90 (MQ=255)                                                               gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       <  1:2227541/90‑1 (MQ=255)                                                               gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       >  1:1508769/1‑90 (MQ=255)                                                               gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       <  2:1452862/90‑1 (MQ=255)                                                               gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       >  1:1519131/1‑90 (MQ=255)                                                               gTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtt                       >  2:1379303/1‑90 (MQ=255)                                                                 ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                       <  1:335466/72‑1 (MQ=255)                                                                 ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                       >  2:335466/1‑72 (MQ=255)                                                                 ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtttt                     >  1:487898/1‑90 (MQ=255)                                                                 ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtttt                     >  2:1423483/1‑90 (MQ=255)                                                                 ttGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGtttt                     >  2:1215605/1‑90 (MQ=255)                                                                       gcAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               >  1:2957825/1‑90 (MQ=255)                                                                       gcAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               >  1:2096466/1‑90 (MQ=255)                                                                       gcAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               <  1:1973476/90‑1 (MQ=255)                                                                       gcAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               >  1:1857440/1‑90 (MQ=255)                                                                       gcAATGAACGCCGAAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               <  1:1379303/90‑1 (MQ=255)                                                                       gaaaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc               <  1:2481877/88‑1 (MQ=255)                                                                         aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag             >  2:783176/1‑90 (MQ=255)                                                                            gAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTga          <  2:109772/90‑1 (MQ=255)                                                                              aCGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGc                                                     >  2:656516/1‑45 (MQ=255)                                                                              aCGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGc                                                     <  1:656516/45‑1 (MQ=255)                                                                              aCGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTgaga        <  1:1215605/90‑1 (MQ=255)                                                                               cGTCGCAAGGCCCTCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGaa       <  1:2612216/90‑1 (MQ=255)                                                                               cGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGaa       <  1:2791923/90‑1 (MQ=255)                                                                                  cGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                              <  1:678471/48‑1 (MQ=255)                                                                                  cGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                              >  2:678471/1‑48 (MQ=255)                                                                                    cAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCaga  <  2:136067/90‑1 (MQ=255)                                                                                        |                                                                                      TGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGA  >  NZ_CP009273/4084566‑4084738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑