Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	566,779	G→T	W144L (TGG→TTG)	ybcL →	Raf kinase inhibitor‑like protein YbcL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	566,779	0	G	T	100.0%	301.0 / NA	75	W144L (TGG→TTG)	ybcL	Raf kinase inhibitor‑like protein YbcL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/28);  total (47/28)

AAGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAA  >  NZ_CP009273/566692‑566865                                                                                        |                                                                                       aaGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATtgg                                                                                      >  1:1470358/1‑90 (MQ=255)  aGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGc                                                                                     >  1:2543336/1‑90 (MQ=255)  aGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGc                                                                                     >  1:1021203/1‑90 (MQ=255)  aGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGc                                                                                     >  2:1593212/1‑90 (MQ=255)  aGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGc                                                                                     >  1:884861/1‑90 (MQ=255)  aGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGc                                                                                     >  1:448606/1‑90 (MQ=255)       gAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTaa                                                                                >  1:2687915/1‑90 (MQ=255)       gAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTaa                                                                                >  2:1517062/1‑90 (MQ=255)       gAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTaa                                                                                >  1:2239735/1‑90 (MQ=255)       gAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTaa                                                                                >  2:2606548/1‑90 (MQ=255)        aaaTGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTaaa                                                                               <  1:1985973/90‑1 (MQ=255)             aTTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTg                                                                          >  1:2951385/1‑90 (MQ=255)              ttttGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGa                                                                         >  1:1911907/1‑90 (MQ=255)                ttGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGaaa                                                                       <  1:243865/90‑1 (MQ=255)                  ggCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAg                                                                     <  2:109732/90‑1 (MQ=255)                    cTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGAt                                                                   <  2:1698918/90‑1 (MQ=255)                      aTGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTc                                                                 >  2:324322/1‑90 (MQ=255)                      aTGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTc                                                                 >  1:2132150/1‑90 (MQ=255)                          tGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGt                                                             <  1:2255528/90‑1 (MQ=255)                           gggTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTa                                                            >  1:312417/1‑90 (MQ=255)                           gggTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTa                                                            >  1:115116/1‑90 (MQ=255)                            ggTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAg                                                           >  2:2732993/1‑90 (MQ=255)                            ggTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAg                                                           <  2:448606/90‑1 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCTCATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  1:203597/1‑90 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTc                                                                 <  1:1712492/80‑1 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTc                                                                 >  2:1712492/1‑80 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  2:1467801/1‑90 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  2:2327881/1‑90 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  1:381520/1‑90 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  1:1751751/1‑90 (MQ=255)                                tggtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTc                                                       >  1:1633392/1‑90 (MQ=255)                                  gtggCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTa                                                     <  2:1021203/90‑1 (MQ=255)                                     gcgcATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACt                                                  >  1:2312570/1‑90 (MQ=255)                                     gcgcATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACt                                                  >  2:2658236/1‑90 (MQ=255)                                     gcgcATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACt                                                  >  1:1677600/1‑90 (MQ=255)                                      cgcATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTc                                                 >  1:2407497/1‑90 (MQ=255)                                       gcATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTcc                                                >  1:2569777/1‑90 (MQ=255)                                            tcctccTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCgg                                           <  1:48615/90‑1 (MQ=255)                                              ctcctAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAg                                         >  2:2386954/1‑90 (MQ=255)                                              ctcctAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAg                                         <  2:1036281/90‑1 (MQ=255)                                              ctcctAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAg                                         >  2:184356/1‑90 (MQ=255)                                               tcctAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGc                                        <  1:1944496/90‑1 (MQ=255)                                                   aaaGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTa                                    >  2:3024934/1‑90 (MQ=255)                                                     aGGAGATAAACCACATCATTACCAGTTTCAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGt                                  <  1:2606548/90‑1 (MQ=255)                                                     aGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGt                                  <  1:1114443/90‑1 (MQ=255)                                                     aGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGt                                  <  2:1714645/90‑1 (MQ=255)                                                     aGGAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGt                                  <  1:3024934/90‑1 (MQ=255)                                                      ggAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 <  1:2536392/90‑1 (MQ=255)                                                      ggAGATAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 <  1:2131323/90‑1 (MQ=255)                                                        agaTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTgg                               >  1:625333/1‑90 (MQ=255)                                                        agaTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTgg                               >  2:2251801/1‑90 (MQ=255)                                                         gaTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 <  2:955716/87‑1 (MQ=255)                                                         gaTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 >  1:955716/1‑87 (MQ=255)                                                         gaTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGt                              >  1:1234485/1‑90 (MQ=255)                                                          aTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGtt                             >  2:1052634/1‑90 (MQ=255)                                                          aTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGtt                             >  2:575735/1‑90 (MQ=255)                                                          aTAAACCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGtt                             >  2:469433/1‑90 (MQ=255)                                                             aaCCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTtata                          <  1:1593212/90‑1 (MQ=255)                                                             aaCCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTtata                          <  2:1315936/90‑1 (MQ=255)                                                             aaCCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTtata                          <  1:2125143/90‑1 (MQ=255)                                                             aaCCACATCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTtata                          <  1:2327881/90‑1 (MQ=255)                                                                 acaTCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCt                      >  1:1511624/1‑90 (MQ=255)                                                                 acaTCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCt                      >  1:1457010/1‑90 (MQ=255)                                                                 acaTCATTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCt                      >  1:763761/1‑90 (MQ=255)                                                                  catcatTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCtt                     <  2:2239735/90‑1 (MQ=255)                                                                      atTACCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATg                 >  2:1010567/1‑90 (MQ=255)                                                                         aCCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 >  1:3007246/1‑71 (MQ=255)                                                                         aCCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGtt                                 <  2:3007246/71‑1 (MQ=255)                                                                         aCCAGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCta              >  2:2735000/1‑90 (MQ=255)                                                                            aGTTTAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCtaata           >  1:478164/1‑90 (MQ=255)                                                                               ttAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGc                                        <  1:717578/58‑1 (MQ=255)                                                                               ttAAAGTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGc                                        >  2:717578/1‑58 (MQ=255)                                                                                    gTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCa   <  2:2312570/90‑1 (MQ=255)                                                                                    gTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCa   >  1:1391742/1‑90 (MQ=255)                                                                                    gTATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCa   <  2:2132150/90‑1 (MQ=255)                                                                                     tATTGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCaa  <  1:2892051/90‑1 (MQ=255)                                                                                        |                                                                                       AAGGCCGAAATGATTTTGGCTATGCTGGGTTTGGTGGCGCATGTCCTCCTAAAGGAGATAAACCACATCATTACCAGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAA  >  NZ_CP009273/566692‑566865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑