Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	601,986	A→G	F285S (TTC→TCC)	ybdK ←	YbdK family carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	601,986	0	A	G	100.0%	291.9 / NA	77	F285S (TTC→TCC)	ybdK	YbdK family carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/31);  total (46/31)

AATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATT  >  NZ_CP009273/601899‑602072                                                                                        |                                                                                       aaTCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGgaa                                                                                      <  1:715079/90‑1 (MQ=255)  aTCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAAc                                                                                     >  2:153202/1‑90 (MQ=255)      cAAGGTATCTTCCGTTAGCGGTCGCCGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 <  2:1757424/90‑1 (MQ=255)      cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 <  2:1479237/90‑1 (MQ=255)      cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 <  2:3001822/90‑1 (MQ=255)      cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 <  1:1494195/90‑1 (MQ=255)              cTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTtat                                                                         >  1:132411/1‑90 (MQ=255)                tCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAc                                                                       >  1:237571/1‑90 (MQ=255)                 ccGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAca                                                                      <  2:907093/90‑1 (MQ=255)                 ccGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAca                                                                      <  2:2521847/90‑1 (MQ=255)                  cGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcag                                                                     >  2:1503868/1‑90 (MQ=255)                   gTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcagc                                                                    >  1:887922/1‑90 (MQ=255)                    ttAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcagca                                                                   >  2:717639/1‑90 (MQ=255)                    ttAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcagca                                                                   >  1:1464699/1‑90 (MQ=255)                    ttAGCGGTCGACGATCTCCAGTGTGCGGATCGGGGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATAcagca                                                                   >  2:1935698/1‑90 (MQ=255)                        cGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTa                                                               <  1:1576594/90‑1 (MQ=255)                        cGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTa                                                               <  1:2354894/90‑1 (MQ=255)                            cgacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCt                                                           >  2:29982/1‑90 (MQ=255)                                    tcCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTg                                                   >  1:2825926/1‑90 (MQ=255)                                      cAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGAt                                                 <  2:2778769/90‑1 (MQ=255)                                        gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa                                                  <  2:2042635/87‑1 (MQ=255)                                        gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa                                                  >  1:2042635/1‑87 (MQ=255)                                        gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGt                                               >  2:1557590/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  2:1031053/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  2:2938983/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  1:957980/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  1:345433/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  1:533380/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  1:562312/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  1:2280989/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  2:2867977/1‑90 (MQ=255)                                          gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt                                             >  2:2458909/1‑90 (MQ=255)                                            gCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 >  2:1814645/1‑52 (MQ=255)                                            gCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGtt                                                                                 <  1:1814645/52‑1 (MQ=255)                                               gATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACg                                        >  1:2454012/1‑90 (MQ=255)                                                    gTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGc                                     <  1:2936570/88‑1 (MQ=255)                                                    gTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGc                                     >  2:2936570/1‑88 (MQ=255)                                                    gTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGt                                   >  1:778626/1‑90 (MQ=255)                                                      gatgaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTc                                 <  1:717639/90‑1 (MQ=255)                                                      gatgaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTc                                 <  2:2517184/90‑1 (MQ=255)                                                       atgaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTcc                                <  2:848264/90‑1 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAAc                                         <  1:692164/79‑1 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAAc                                         >  2:692164/1‑79 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTcc                                <  2:1100412/88‑1 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTcc                                >  1:1100412/1‑88 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                              >  1:1356900/1‑90 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                              >  1:909808/1‑90 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                              >  1:1439650/1‑90 (MQ=255)                                                         gaCGCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt                              >  1:722746/1‑90 (MQ=255)                                                            gCCTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAg                           <  2:2207157/90‑1 (MQ=255)                                                             ccTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAGACGGGCGTTCCGTCAGt                          >  1:1391864/1‑90 (MQ=255)                                                             ccTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGt                          >  2:803584/1‑90 (MQ=255)                                                             ccTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGt                          >  2:2354584/1‑90 (MQ=255)                                                              cTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         <  2:1624963/90‑1 (MQ=255)                                                              cTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         <  2:681852/90‑1 (MQ=255)                                                              cTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         >  1:1513079/1‑90 (MQ=255)                                                              cTTCAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         >  2:2714379/1‑90 (MQ=255)                                                                 cAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                      <  2:909808/90‑1 (MQ=255)                                                                 cAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                      <  2:2994302/90‑1 (MQ=255)                                                                 cAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                      <  2:2714154/90‑1 (MQ=255)                                                                 cAAGCCCATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAAcc                      <  1:803584/90‑1 (MQ=255)                                                                     cccATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTg                  <  1:133200/90‑1 (MQ=255)                                                                     cccATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTg                  <  2:2160425/90‑1 (MQ=255)                                                                       cATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTggg                >  2:266674/1‑90 (MQ=255)                                                                       cATAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTggg                >  2:1263016/1‑90 (MQ=255)                                                                        aTAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         <  2:2736957/80‑1 (MQ=255)                                                                        aTAGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa                         >  1:2736957/1‑80 (MQ=255)                                                                          aGCGACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCgg             <  2:132411/90‑1 (MQ=255)                                                                             gACAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAg          >  1:1853730/1‑90 (MQ=255)                                                                              aCAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGc         >  1:2440053/1‑90 (MQ=255)                                                                               cAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc        <  1:266674/90‑1 (MQ=255)                                                                               cAGGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc        <  2:2784162/90‑1 (MQ=255)                                                                                 ggCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa                                                  >  2:2176524/1‑46 (MQ=255)                                                                                 ggCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa                                                  <  1:2176524/46‑1 (MQ=255)                                                                                 ggCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTg      >  2:2022200/1‑90 (MQ=255)                                                                                 ggCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTg      >  1:815754/1‑90 (MQ=255)                                                                                    cTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAt   >  1:1380208/1‑90 (MQ=255)                                                                                     tGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAtt  >  1:1502961/1‑90 (MQ=255)                                                                                        |                                                                                       AATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACAGGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAATT  >  NZ_CP009273/601899‑602072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑