Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	874,237	C→T	L14F (CTT→TTT)	yliI →	aldose sugar dehydrogenase YliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	874,237	0	C	T	100.0%	342.4 / NA	85	L14F (CTT→TTT)	yliI	aldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/39);  total (46/39)

ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGG  >  NZ_CP009273/874150‑874318                                                                                        |                                                                                  aTCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGtttt                                                                                 <  1:950026/90‑1 (MQ=255)     cctccACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCTTTTTTCCTTGTGCCCCTTATTTGtttttctt                                                                             >  1:1422359/1‑90 (MQ=255)        ccACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCg                                                                          >  1:5475/1‑90 (MQ=255)        ccACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCg                                                                          <  2:2464582/90‑1 (MQ=255)           cTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctc                                                                       >  2:2208491/1‑90 (MQ=255)             gACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctc                                                                     >  1:988480/1‑90 (MQ=255)             gACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctc                                                                     >  1:605162/1‑90 (MQ=255)             gACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctc                                                                     >  1:2569522/1‑90 (MQ=255)             gACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctc                                                                     >  1:2421413/1‑90 (MQ=255)              aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGctctct                                                                    <  2:5475/90‑1 (MQ=255)                tACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTgg                                                                  >  2:2330492/1‑90 (MQ=255)                  cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGggc                                                                <  2:605162/90‑1 (MQ=255)                  cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGggc                                                                >  2:1193086/1‑90 (MQ=255)                  cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGggc                                                                >  1:2324990/1‑90 (MQ=255)                   gCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGggcg                                                               <  2:1422359/90‑1 (MQ=255)                       tAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTcc                                                                           <  2:1078558/74‑1 (MQ=255)                       tAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTcc                                                                           >  1:1078558/1‑74 (MQ=255)                       tAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTc                                                           >  2:1537415/1‑90 (MQ=255)                         aGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCt                                                         >  1:2698158/1‑90 (MQ=255)                            cAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCa                                                      <  1:1213595/90‑1 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                                    >  2:1773697/1‑90 (MQ=255)                              gagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAAc                                                    >  2:2925556/1‑90 (MQ=255)                                  cAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTa                                                <  2:1986652/90‑1 (MQ=255)                                  cAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTa                                                <  1:265362/90‑1 (MQ=255)                                  cAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTa                                                >  2:2378215/1‑90 (MQ=255)                                  cAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTa                                                <  1:1794448/90‑1 (MQ=255)                                   aaTCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaa                                               >  1:1749731/1‑90 (MQ=255)                                    aTCCGAAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaaa                                              >  1:2786193/1‑90 (MQ=255)                                     tCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAAt                                             <  2:2421413/90‑1 (MQ=255)                                         gAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCg                                         >  1:2720086/1‑90 (MQ=255)                                          aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGa                                        <  1:1773697/90‑1 (MQ=255)                                          aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGa                                        >  1:3014043/1‑90 (MQ=255)                                             cGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     <  2:1523032/90‑1 (MQ=255)                                             cGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     <  2:1608072/90‑1 (MQ=255)                                             cGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     <  1:1328906/90‑1 (MQ=255)                                             cGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     <  1:676852/90‑1 (MQ=255)                                              gTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACg                                                   <  1:2293294/75‑1 (MQ=255)                                              gTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACg                                                   >  2:2293294/1‑75 (MQ=255)                                              gTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTa                                    >  2:575013/1‑90 (MQ=255)                                              gTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTa                                    >  2:900180/1‑90 (MQ=255)                                              gTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTa                                    >  1:2397876/1‑90 (MQ=255)                                                  tGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGc                                <  1:575013/90‑1 (MQ=255)                                                  tGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGc                                <  2:2149323/90‑1 (MQ=255)                                                  tGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGc                                <  1:2907103/90‑1 (MQ=255)                                                      tCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     <  2:2323759/81‑1 (MQ=255)                                                      tCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGt                                     >  1:2323759/1‑81 (MQ=255)                                                      tCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGa                            >  2:1850804/1‑90 (MQ=255)                                                      tCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGa                            >  1:2832755/1‑90 (MQ=255)                                                        gACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACa                          >  1:475683/1‑90 (MQ=255)                                                        gACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACa                          >  1:1753725/1‑90 (MQ=255)                                                        gACAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACa                          >  2:227615/1‑90 (MQ=255)                                                         aCAATCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACaa                         >  1:2406819/1‑90 (MQ=255)                                                           aaTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAAc                       >  1:992751/1‑90 (MQ=255)                                                           aaTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAAc                       >  2:1586517/1‑90 (MQ=255)                                                           aaTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAAc                       <  1:164406/90‑1 (MQ=255)                                                            aTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTcc                                                                           >  1:1574161/1‑37 (MQ=38)                                                            aTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTcc                                                                           <  2:1574161/37‑1 (MQ=38)                                                            aTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCt                                                            <  2:2939308/52‑1 (MQ=255)                                                            aTCCTTTTTCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCt                                                            >  1:2939308/1‑52 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:2813333/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:1174361/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  2:475683/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:1586517/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:2212565/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  2:1749731/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:2925556/90‑1 (MQ=255)                                                                 ttttCCTTGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:985345/90‑1 (MQ=255)                                                                       ttGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCt           >  1:2926394/1‑90 (MQ=255)                                                                       ttGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCt           >  1:1559670/1‑90 (MQ=255)                                                                       ttGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCt           >  1:343693/1‑90 (MQ=255)                                                                       ttGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCt           >  1:50511/1‑90 (MQ=255)                                                                       ttGTGCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCt           >  2:319575/1‑90 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 >  2:2042221/1‑83 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:2042221/83‑1 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTg          <  1:1330648/90‑1 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTg          <  2:1440863/90‑1 (MQ=255)                                                                        tgtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTg          <  1:2330492/90‑1 (MQ=255)                                                                         gtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                     >  2:1305989/1‑46 (MQ=255)                                                                         gtgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                     <  1:1305989/46‑1 (MQ=255)                                                                          tgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaa                                               <  1:425722/51‑1 (MQ=255)                                                                          tgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTaa                                               >  2:425722/1‑51 (MQ=255)                                                                          tgCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTggg        >  2:430157/1‑90 (MQ=255)                                                                           gCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAGATGTCGAAGTACTGCAAGACAAACTCGAcc                 <  1:1890947/80‑1 (MQ=255)                                                                           gCCCCTTATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAGATGTCGAAGTACTGCAAGACAAACTCGAcc                 >  2:1890947/1‑80 (MQ=255)                                                                                ttATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                     <  1:2052451/39‑1 (MQ=39)                                                                                ttATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                     >  2:2052451/1‑39 (MQ=39)                                                                                ttATTTGTTTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTgg  >  1:2131609/1‑90 (MQ=255)                                                                                        |                                                                                  ATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGG  >  NZ_CP009273/874150‑874318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑