Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,062,210	T→C	G390G (GGT→GGC)	agp →	bifunctional glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,062,210	0	T	C	100.0%	298.0 / NA	79	G390G (GGT→GGC)	agp	bifunctional glucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/35);  total (44/35)

TGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCC  >  NZ_CP009273/1062123‑1062293                                                                                        |                                                                                    tgtgTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTg                                                                                   >  1:642790/1‑90 (MQ=255)  gtgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGc                                                                                  <  1:1220774/90‑1 (MQ=255)   tgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGcc                                                                                 <  1:886650/90‑1 (MQ=255)         agagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAg                                                                           <  2:14778/90‑1 (MQ=255)         agagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAg                                                                           <  2:183666/90‑1 (MQ=255)           agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGAc                                                                         <  1:2399560/90‑1 (MQ=255)           agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGAc                                                                         >  1:1838604/1‑90 (MQ=255)            gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                        >  1:1688098/1‑90 (MQ=255)            gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                        >  1:522652/1‑90 (MQ=255)            gTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACg                                                                        >  2:960083/1‑90 (MQ=255)             tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGc                                                                       <  1:2483462/90‑1 (MQ=255)             tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGc                                                                       <  2:2765201/90‑1 (MQ=255)              gCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGcc                                                                                 <  2:481690/79‑1 (MQ=255)              gCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGcc                                                                                 >  1:481690/1‑79 (MQ=255)              gCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCt                                                                      >  2:2794778/1‑90 (MQ=255)                 gAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGAt                                                                   <  2:1838604/90‑1 (MQ=255)                 gAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGAt                                                                   <  1:1235118/90‑1 (MQ=255)                 gAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGAt                                                                   <  2:349801/90‑1 (MQ=255)                     aGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGtt                                                               <  1:264600/90‑1 (MQ=255)                      gTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGttt                                                              >  1:1156414/1‑90 (MQ=255)                      gTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGttt                                                              >  2:1881084/1‑90 (MQ=255)                        tACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCt                                                            >  2:2956433/1‑90 (MQ=255)                        tACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCt                                                            >  2:1722037/1‑90 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGc                                                                                  <  1:358334/62‑1 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGc                                                                                  >  2:358334/1‑62 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      >  1:1029495/1‑90 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      >  2:2883869/1‑90 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      >  1:2268337/1‑90 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      <  1:1831409/90‑1 (MQ=255)                              aTGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      >  1:140840/1‑90 (MQ=255)                                gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                    >  2:21820/1‑90 (MQ=255)                                gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                    >  2:1063184/1‑90 (MQ=255)                                gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                    >  1:404265/1‑90 (MQ=255)                                gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                    >  2:350246/1‑90 (MQ=255)                                gCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                    >  2:92507/1‑90 (MQ=255)                                  cGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGa                                                  <  2:140840/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  1:779950/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  2:1688098/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  2:561939/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  2:2631763/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  2:2723856/90‑1 (MQ=255)                                    aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                >  1:2631763/1‑90 (MQ=255)                                      gCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAg                                              <  2:1544880/90‑1 (MQ=255)                                             cccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                       <  1:1133917/90‑1 (MQ=255)                                              ccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAg                                      <  2:2441588/90‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAGTTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  1:2232312/78‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAGTTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  1:2351517/78‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAGTTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                >  2:2351517/1‑78 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAGTTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                >  2:2232312/1‑78 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                >  1:2394888/1‑78 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATa                                                <  2:2394888/78‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                       <  2:1551689/87‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                       >  1:1551689/1‑87 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCg                                    >  1:1195359/1‑90 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCg                                    <  2:382926/90‑1 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCg                                    >  1:2711478/1‑90 (MQ=255)                                                tGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCg                                    >  1:2987013/1‑90 (MQ=255)                                                   aGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCgtgt                                 <  1:2297857/90‑1 (MQ=255)                                                      cACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTGTCTGCCCGATGGATAAGTTTGATAGCGTGTtga                              >  2:2262929/1‑90 (MQ=255)                                                         cTGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatg                           >  1:900565/1‑90 (MQ=255)                                                          tGCGCAGCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatga                          <  2:1075302/90‑1 (MQ=255)                                                                gCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAg                                                                           >  2:2412091/1‑35 (MQ=255)                                                                gCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAg                                                                           <  1:2412091/35‑1 (MQ=255)                                                                gCGTGTGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGt                    >  1:2963983/1‑90 (MQ=255)                                                                  gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGccc                                                        <  1:2688365/52‑1 (MQ=255)                                                                  gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGccc                                                        >  2:2688365/1‑52 (MQ=255)                                                                  gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCgtgt                                 >  1:2326343/1‑75 (MQ=255)                                                                  gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCgtgt                                 <  2:2326343/75‑1 (MQ=255)                                                                  gtgtGACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGa                  >  2:2187561/1‑90 (MQ=255)                                                                     tgACGCTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAAt               <  2:2493930/90‑1 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      >  1:874318/1‑46 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGa                                                      <  2:874318/46‑1 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg          >  1:2210894/1‑90 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg          >  1:1866464/1‑90 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAg          >  1:1264515/1‑90 (MQ=255)                                                                          cTGGAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGAGAGCGAGTTGAATGAAGCTGTGAAATAACAg          >  1:1943647/1‑90 (MQ=255)                                                                            ggAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGaa        >  1:900518/1‑90 (MQ=255)                                                                            ggAATTAAGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGaa        >  2:433715/1‑90 (MQ=255)                                                                                  aaGCGGCTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcc  >  2:1850440/1‑90 (MQ=255)                                                                                        |                                                                                    TGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCC  >  NZ_CP009273/1062123‑1062293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑