Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,156,712	C→G	G99A (GGC→GCC)	fhuE ←	ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,156,712	0	C	G	100.0%	239.9 / NA	64	G99A (GGC→GCC)	fhuE	ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (33/31);  total (33/31)

TCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGC  >  NZ_CP009273/1156625‑1156795                                                                                        |                                                                                    tCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGgcc                                                                                   <  1:93838/90‑1 (MQ=255)  cGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCa                                                                                  >  2:2706789/1‑90 (MQ=255)   gATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAg                                                                                 <  2:2938105/90‑1 (MQ=255)     tCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCg                                                                               >  2:400373/1‑90 (MQ=255)      cTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGt                                                                              <  2:2326377/90‑1 (MQ=255)        ggAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGttt                                                                            <  2:552622/90‑1 (MQ=255)              cgcgGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAAct                                                                      <  1:2995953/90‑1 (MQ=255)               gcgGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActg                                                                     >  2:1186327/1‑90 (MQ=255)                cgGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgc                                                                    >  1:2972758/1‑90 (MQ=255)                cgGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgc                                                                    >  2:1868707/1‑90 (MQ=255)                  ggAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  <  1:751571/90‑1 (MQ=255)                  ggAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  >  2:1065317/1‑90 (MQ=255)                  ggAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  <  1:2815371/90‑1 (MQ=255)                  ggAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  <  1:1558283/90‑1 (MQ=255)                       aataaAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  >  1:1530125/1‑85 (MQ=255)                       aataaAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAActgctg                                                                  <  2:1530125/85‑1 (MQ=255)                            agagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATc                                                        >  2:2663296/1‑90 (MQ=255)                            agagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATc                                                        >  2:2427354/1‑90 (MQ=255)                             gagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTa                                                                         <  2:2794056/72‑1 (MQ=255)                             gagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTa                                                                         >  1:2794056/1‑72 (MQ=255)                             gagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATcc                                                       >  2:1563787/1‑90 (MQ=255)                             gagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATcc                                                       >  2:1578766/1‑90 (MQ=255)                             gagCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATcc                                                       >  2:353320/1‑90 (MQ=255)                               gCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCgc                                                     >  1:2046350/1‑90 (MQ=255)                               gCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCgc                                                     >  2:348383/1‑90 (MQ=255)                                  cGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCgctgc                                                  <  2:1320973/90‑1 (MQ=255)                                  cGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCgctgc                                                  >  1:1876370/1‑90 (MQ=255)                                  cGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCgctgc                                                  <  1:906406/90‑1 (MQ=255)                                     tCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTgg                                               <  1:2222750/90‑1 (MQ=255)                                     tCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTgg                                               <  1:2203563/90‑1 (MQ=255)                                     tCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTgg                                               <  1:97579/90‑1 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAAct                                                                      >  2:760750/1‑65 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAAct                                                                      <  1:760750/65‑1 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATc                                                               <  2:2686741/72‑1 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATc                                                               >  1:2686741/1‑72 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCt                                             <  1:1219593/90‑1 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCt                                             <  1:1345581/90‑1 (MQ=255)                                       ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCt                                             >  1:2693359/1‑90 (MQ=255)                                         aaTCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTaa                                           >  1:676816/1‑90 (MQ=255)                                         aaTCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTaa                                           <  1:400373/90‑1 (MQ=255)                                         aaTCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTaa                                           <  2:2849088/90‑1 (MQ=255)                                             cGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCCCTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAAt                                       <  1:1792965/90‑1 (MQ=255)                                              gCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATa                                      >  1:2971611/1‑90 (MQ=255)                                              gCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATa                                      >  2:2670787/1‑90 (MQ=255)                                                 tGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTg                                   >  2:335195/1‑90 (MQ=255)                                                    cTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGAcc                                <  2:2898395/90‑1 (MQ=255)                                                    cTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGAcc                                <  1:1578766/90‑1 (MQ=255)                                                       ttGCTGATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGAc                             <  2:1670200/90‑1 (MQ=255)                                                            gATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAAt                                       >  2:1430763/1‑75 (MQ=255)                                                            gATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAAt                                       <  1:1430763/75‑1 (MQ=255)                                                            gATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAgg                        <  2:1876370/90‑1 (MQ=255)                                                            gATCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAgg                        >  1:2614484/1‑90 (MQ=255)                                                              tCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGaa                      >  1:240831/1‑90 (MQ=255)                                                              tCCCCAGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGaa                      >  1:612732/1‑90 (MQ=255)                                                                   aGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCa                 <  1:1868707/90‑1 (MQ=255)                                                                   aGCGTGTTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCa                 >  1:2534030/1‑90 (MQ=255)                                                                      gtgtTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAatat                   >  1:1510564/1‑85 (MQ=255)                                                                      gtgtTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAatat                   <  2:1510564/85‑1 (MQ=255)                                                                      gtgtTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGt              >  2:2509484/1‑90 (MQ=255)                                                                        gtTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTg            >  1:769545/1‑90 (MQ=255)                                                                        gtTTTCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGGGACCGACTGAGGAATATCACGTTg            >  1:1717554/1‑90 (MQ=255)                                                                          tttCCATCACTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAg          <  2:2521210/90‑1 (MQ=255)                                                                                  aCTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTaa                                           >  2:1345429/1‑49 (MQ=255)                                                                                  aCTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTaa                                           <  1:1345429/49‑1 (MQ=255)                                                                                  aCTTCGGCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGc  <  1:2509484/90‑1 (MQ=255)                                                                                        |                                                                                    TCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGC  >  NZ_CP009273/1156625‑1156795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑