Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,331,016	A→G	Y310C (TAT→TGT)	acnA →	aconitate hydratase AcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,331,016	0	A	G	100.0%	227.2 / NA	61	Y310C (TAT→TGT)	acnA	aconitate hydratase AcnA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/38);  total (23/38)

GAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAG  >  NZ_CP009273/1330933‑1331101                                                                                    |                                                                                      gAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGc                                                                                 <  1:119018/90‑1 (MQ=255) gAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGc                                                                                 >  2:1347147/1‑90 (MQ=255) gAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGc                                                                                 <  2:2744021/90‑1 (MQ=255)  aaaTTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGcc                                                                                <  1:2398848/90‑1 (MQ=255)   aaTTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCa                                                                               >  2:2315797/1‑90 (MQ=255)        gtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCtgt                                                                          <  1:2315797/90‑1 (MQ=255)        gtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCtgt                                                                          <  1:1917099/90‑1 (MQ=255)            aaTTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGct                                                                      <  2:2163078/90‑1 (MQ=255)            aaTTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGct                                                                      <  1:2238920/90‑1 (MQ=255)            aaTTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAACATGTCGCCAGAATGTGGTGCCACCTGTGGct                                                                      <  2:2261462/90‑1 (MQ=255)              ttttATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGcttc                                                                    <  1:1536202/90‑1 (MQ=255)                  aTGGTGATGGTCTGGATTCACTACCGTTTGCGGATCGCGCCACCATTGCAAATATGTCGCCTGAAGGTGGTGCCTTCCGTGGCTTGTTcc                                                                <  1:3008604/90‑1 (MQ=255)                       gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGct                                                                      >  1:2611706/1‑79 (MQ=255)                       gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGct                                                                      <  2:2611706/79‑1 (MQ=255)                       gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                                           >  2:1895501/1‑90 (MQ=255)                          ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  1:2573293/90‑1 (MQ=255)                          ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  1:2338709/90‑1 (MQ=255)                          ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  1:22750/90‑1 (MQ=255)                          ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  2:674276/90‑1 (MQ=255)                          ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  2:2879894/90‑1 (MQ=255)                               ggATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                   >  1:2787976/1‑90 (MQ=255)                                gATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                  <  2:2295826/90‑1 (MQ=255)                                 aTTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        <  2:2502797/83‑1 (MQ=255)                                 aTTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                        >  1:2502797/1‑83 (MQ=255)                                 aTTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTaa                                                 <  1:207287/90‑1 (MQ=255)                                  ttCACTACCGTTGGCGGATCGCGCCACCTTTGCCAATATGCCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAc                                                <  1:1430705/90‑1 (MQ=255)                                   tCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAcc                                               <  1:744061/90‑1 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                                           <  2:1040886/77‑1 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATc                                                           >  1:1040886/1‑77 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGa                                                         >  2:1558743/1‑79 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGa                                                         <  1:1558743/79‑1 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAccc                                              >  1:924873/1‑90 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAccc                                              >  1:1408185/1‑90 (MQ=255)                                    cACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAccc                                              >  2:1054760/1‑90 (MQ=255)                                     aCTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                             <  1:2030199/90‑1 (MQ=255)                                     aCTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                             <  1:160159/90‑1 (MQ=255)                                      cTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTc                                            <  2:2787976/90‑1 (MQ=255)                                         ccGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAt                                         >  1:211642/1‑90 (MQ=255)                                         ccGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAt                                         >  2:2201564/1‑90 (MQ=255)                                           gTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTa                                       >  1:2790515/1‑90 (MQ=255)                                             tGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                     <  2:2499783/90‑1 (MQ=255)                                             tGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                     <  1:1250640/90‑1 (MQ=255)                                             tGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                     <  1:2465962/90‑1 (MQ=255)                                              ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                     >  1:503345/1‑89 (MQ=255)                                              ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACa                                     <  2:503345/89‑1 (MQ=255)                                              ggCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACAt                                    <  2:566928/90‑1 (MQ=255)                                                    tCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGttt                              >  2:2930450/1‑90 (MQ=255)                                                    tCGCGCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGttt                              >  2:776123/1‑90 (MQ=255)                                                     cgcgCCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTa                             >  1:1741438/1‑90 (MQ=255)                                                        gcCACCATTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc                          <  2:2067567/90‑1 (MQ=255)                                                              aTTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                    <  1:2239523/90‑1 (MQ=255)                                                              aTTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                    <  2:264441/90‑1 (MQ=255)                                                              aTTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                    <  2:456977/90‑1 (MQ=255)                                                              aTTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                    <  1:1465560/90‑1 (MQ=255)                                                              aTTGCCAATATGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                    <  1:1338858/90‑1 (MQ=255)                                                                     atatGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAg             >  1:1898690/1‑90 (MQ=255)                                                                        tGTCGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc          <  2:1234661/90‑1 (MQ=255)                                                                           cGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAgg       >  1:808962/1‑90 (MQ=255)                                                                           cGCCAGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAgg       >  1:160188/1‑90 (MQ=255)                                                                               aGAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGa   >  2:1793691/1‑90 (MQ=255)                                                                                gAATGTGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAg  >  2:469472/1‑90 (MQ=255)                                                                                    |                                                                                      GAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAG  >  NZ_CP009273/1330933‑1331101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑