Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,434,377	A→G	F222S (TTC→TCC)	nifJ ←	pyruvate:ferredoxin (flavodoxin) oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,434,377	0	A	G	100.0%	360.7 / NA	94	F222S (TTC→TCC)	nifJ	pyruvate:ferredoxin (flavodoxin) oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (63/31);  total (63/31)

CCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCG  >  NZ_CP009273/1434290‑1434460                                                                                        |                                                                                    ccTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGgaa                                                                                   >  1:2637621/1‑90 (MQ=255)   tGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 <  2:527439/90‑1 (MQ=255)   tGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 <  1:2074742/90‑1 (MQ=255)           cAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAg                                                                         >  1:394566/1‑90 (MQ=255)              agaAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGAt                                                                      <  2:2642735/90‑1 (MQ=255)                  aTCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGc                                                                  <  2:1946591/90‑1 (MQ=255)                            gCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACcg                                                        >  1:2561721/1‑90 (MQ=255)                             ccTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          <  1:602900/87‑1 (MQ=255)                             ccTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          >  2:602900/1‑87 (MQ=255)                                gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa                                                    >  1:750609/1‑90 (MQ=255)                                gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa                                                    >  2:1920826/1‑90 (MQ=255)                                gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGGACCGCGGa                                                    >  1:1197824/1‑90 (MQ=255)                                gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCAAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa                                                    <  2:177438/90‑1 (MQ=255)                                        tGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  1:1041597/90‑1 (MQ=255)                                        tGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  2:2331465/90‑1 (MQ=255)                                        tGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  1:1026583/90‑1 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 <  2:2157577/51‑1 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 >  1:2157577/1‑51 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          >  1:2684740/1‑74 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          <  2:2684740/74‑1 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg                                          >  2:1770558/1‑90 (MQ=255)                                          gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg                                          >  2:1223072/1‑90 (MQ=255)                                             aTAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTc                                       <  2:1432146/90‑1 (MQ=255)                                             aTAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTc                                       <  2:729122/90‑1 (MQ=255)                                              tAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTcc                                      <  1:122814/90‑1 (MQ=255)                                               aGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCg                                     >  1:2196946/1‑90 (MQ=255)                                                  ccGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGt                                  >  1:1813791/1‑90 (MQ=255)                                                  ccGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGt                                  >  1:2862292/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATaa                                                                                <  2:2709189/42‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATaa                                                                                >  1:2709189/1‑42 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 >  2:1764216/1‑41 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa                                                                                 <  1:1764216/41‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGa                                                               <  1:988618/59‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGa                                                               >  2:988618/1‑59 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  1:1680350/78‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            >  2:1680350/1‑78 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg                                          <  1:2985353/80‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg                                          >  2:2985353/1‑80 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:1234680/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                <  2:2564768/90‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:1975325/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:1553783/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:1511258/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:2731463/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:1772808/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:2076236/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                <  2:1553783/90‑1 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:950919/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:1500165/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:1367121/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:2816763/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:1045465/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:395832/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:740954/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  2:691639/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:540383/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:2564768/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:644458/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:2458236/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg                                >  1:912695/1‑90 (MQ=255)                                                    gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGGTg                                >  2:2484675/1‑90 (MQ=255)                                                           aCCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGccc                         <  1:2221667/90‑1 (MQ=255)                                                           aCCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGccc                         <  1:2283804/90‑1 (MQ=255)                                                              aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          <  1:661407/54‑1 (MQ=255)                                                              aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAc                                                          >  2:661407/1‑54 (MQ=255)                                                              aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCggg                      >  2:1636042/1‑90 (MQ=255)                                                              aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCggg                      >  2:42138/1‑90 (MQ=255)                                                                 ggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgcgc                   >  2:2862256/1‑90 (MQ=255)                                                                 ggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGACCGGgcgc                   >  1:2252676/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGATGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  1:171043/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            >  1:2591520/1‑63 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  2:2591520/63‑1 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgc                     <  1:1331421/86‑1 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgc                     >  2:1331421/1‑86 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  2:939510/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  1:1624747/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  2:773988/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  1:2031288/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  2:2325146/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  1:824005/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  2:661701/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGGTGAGTGCCCGGGCGCGa                 >  1:427946/1‑90 (MQ=255)                                                                   ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTAGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa                 >  2:1833998/1‑90 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            >  2:2306680/1‑61 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa                                            <  1:2306680/61‑1 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGt                                  >  2:583441/1‑71 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGt                                  <  1:583441/71‑1 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATg               >  1:1699615/1‑90 (MQ=255)                                                                     ggtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATg               >  1:2590183/1‑90 (MQ=255)                                                                      gtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGa              <  1:42138/90‑1 (MQ=255)                                                                        ggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGc            >  2:65574/1‑90 (MQ=255)                                                                           ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc         >  2:1539260/1‑90 (MQ=255)                                                                           ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc         <  1:1975325/90‑1 (MQ=255)                                                                                gagaCTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAAttt    <  1:919433/90‑1 (MQ=255)                                                                                  gaCTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCg  >  2:819437/1‑90 (MQ=255)                                                                                        |                                                                                    CCTGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCG  >  NZ_CP009273/1434290‑1434460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑