Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,494,123	T→C	E195G (GAA→GGA)	ydcK ←	YdcK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,494,123	0	T	C	100.0%	235.0 / NA	62	E195G (GAA→GGA)	ydcK	YdcK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

GCATCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTG  >  NZ_CP009273/1494036‑1494208                                                                                        |                                                                                      gCATCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCa                                                                                     >  1:1479279/1‑90 (MQ=255)   aTCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAAt                                                                                   >  1:1146855/1‑90 (MQ=255)            tATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaa                                                                          >  1:1981360/1‑90 (MQ=255)            tATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaa                                                                          <  2:1919512/90‑1 (MQ=255)                  aGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaa                                                                    >  1:126132/1‑90 (MQ=255)                   gCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaaa                                                                   >  1:802306/1‑90 (MQ=255)                   gCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaaa                                                                   >  1:337327/1‑90 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         >  1:62012/1‑90 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         >  1:1166077/1‑90 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         >  1:567244/1‑90 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         <  2:785261/90‑1 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         >  2:1123518/1‑90 (MQ=255)                             gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc                                                         >  2:172100/1‑90 (MQ=255)                              cgcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGcgcg                                                        >  2:492897/1‑90 (MQ=255)                               gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGa                                                       <  1:649872/90‑1 (MQ=255)                               gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGa                                                       <  2:1146855/90‑1 (MQ=255)                                cgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTTCCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGAt                                                      <  2:720952/90‑1 (MQ=255)                                   gACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGtt                                                   <  1:2690520/90‑1 (MQ=255)                                   gACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGtt                                                   >  1:1081306/1‑90 (MQ=255)                                    aCCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTc                                                  >  2:823271/1‑90 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATGCTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               <  1:1557483/90‑1 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               >  2:361525/1‑90 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               <  2:1705534/90‑1 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               >  2:1390034/1‑90 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               >  1:1771845/1‑90 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               >  1:96612/1‑90 (MQ=255)                                       gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               <  1:592047/90‑1 (MQ=255)                                           aCTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATaaaa                                           <  2:55742/90‑1 (MQ=255)                                             tttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGc                                         <  2:2186002/90‑1 (MQ=255)                                              ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCa                                        >  2:944658/1‑90 (MQ=255)                                              ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCa                                        >  1:193622/1‑90 (MQ=255)                                               tAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               >  1:2170343/1‑82 (MQ=255)                                               tAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                               <  2:2170343/82‑1 (MQ=255)                                                      aTCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgac                                >  2:743115/1‑90 (MQ=255)                                                      aTCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgac                                >  1:1924272/1‑90 (MQ=255)                                                               ccAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                       <  1:114992/90‑1 (MQ=255)                                                               ccAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                       <  1:1390034/90‑1 (MQ=255)                                                               ccAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                       <  1:2186331/90‑1 (MQ=255)                                                                  gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGcc                    >  1:2440612/1‑90 (MQ=255)                                                                  gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGcc                    >  2:1080346/1‑90 (MQ=255)                                                                   aCATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCa                   <  2:1166077/90‑1 (MQ=255)                                                                     aTTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATa                 <  2:78200/90‑1 (MQ=255)                                                                      ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa                >  2:621379/1‑90 (MQ=255)                                                                      ttGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATaa                <  1:1260177/90‑1 (MQ=255)                                                                        gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt              >  1:460437/1‑90 (MQ=255)                                                                             ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                       <  2:2160596/76‑1 (MQ=255)                                                                             ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                       >  1:2160596/1‑76 (MQ=255)                                                                             ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg         <  1:836824/90‑1 (MQ=255)                                                                             ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg         >  2:1142506/1‑90 (MQ=255)                                                                              ttCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGc        >  1:836609/1‑90 (MQ=255)                                                                              ttCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGc        >  1:268074/1‑90 (MQ=255)                                                                                 aTTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtg     <  2:2440612/90‑1 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGt                                                    >  1:393443/1‑42 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGt                                                    <  2:393443/42‑1 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    >  2:851097/1‑90 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    <  1:540304/90‑1 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    >  1:176867/1‑90 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    >  2:2321883/1‑90 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    >  2:2044654/1‑90 (MQ=255)                                                                                  ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg    >  1:2856392/1‑90 (MQ=255)                                                                                   tACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggt   <  1:2282079/90‑1 (MQ=255)                                                                                    aCCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggtg  <  2:1223723/90‑1 (MQ=255)                                                                                        |                                                                                      GCATCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTG  >  NZ_CP009273/1494036‑1494208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑