Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	130,698	A→T	*866L (TAA→TTA)	acnB →	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	130,698	0	A	T	100.0%	381.1 / NA	93	*866L (TAA→TTA)	acnB	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (55/38);  total (55/38)

CCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAT  >  NZ_CP009273/130611‑130780                                                                                        |                                                                                   ccGTTGGTACGTCCCGCTATCTGATCTTCAACCAGCTTTCTCAGTACACCGGGAAAGCCCATGGGGTGATTTGCCAGACTGCGGTTTtaa                                                                                  <  1:1234610/90‑1 (MQ=255) ccGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTtaa                                                                                  >  1:2117445/1‑90 (MQ=255)     tGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGt                                                                              >  1:2097735/1‑90 (MQ=255)         aCTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAgc                                                                          <  2:1996305/90‑1 (MQ=255)            tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCa                                                                       >  2:1892720/1‑90 (MQ=255)              ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAcg                                                                     <  2:470369/90‑1 (MQ=255)               cGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAcgc                                                                    <  1:1280240/90‑1 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  <  2:985739/90‑1 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  <  1:519599/90‑1 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  <  2:2316293/90‑1 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  >  1:2098912/1‑90 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  <  2:984978/90‑1 (MQ=255)                 ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc                                                                  <  1:1475263/90‑1 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:2853011/1‑90 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 <  1:262455/90‑1 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:262455/1‑90 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:504926/1‑90 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:2509344/1‑90 (MQ=255)                  tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 <  1:957994/90‑1 (MQ=255)                   aTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTg                                                                >  1:2894966/1‑90 (MQ=255)                   aTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTg                                                                >  1:1345552/1‑90 (MQ=255)                       gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 <  1:1914604/85‑1 (MQ=255)                       gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:1914604/1‑85 (MQ=255)                       gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCa                                                            <  2:784186/90‑1 (MQ=255)                       gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCa                                                            <  1:2610629/90‑1 (MQ=255)                       gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCa                                                            <  1:2862548/90‑1 (MQ=255)                        aaCTTCAACCAGCTTTCTCAGTTCACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCAt                                                           >  1:1524937/1‑90 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  1:1480398/1‑80 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 <  2:1480398/80‑1 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       <  2:1312091/90‑1 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       <  1:1050829/90‑1 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       <  2:148804/90‑1 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       >  2:881121/1‑90 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       <  2:2439388/90‑1 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc                                                       >  2:257504/1‑90 (MQ=255)                            tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGA‑TTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACg                                                      >  2:1650325/1‑90 (MQ=255)                             cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACg                                                      <  1:1892720/90‑1 (MQ=255)                                 cAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                  <  2:407558/90‑1 (MQ=255)                                  aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGAcaca                                                 <  2:482053/90‑1 (MQ=255)                                    cTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGa                                                     >  1:423257/1‑84 (MQ=255)                                    cTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGa                                                     <  2:423257/84‑1 (MQ=255)                                      ttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGc                                             >  1:205387/1‑90 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  1:2241526/90‑1 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  2:2834503/90‑1 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  2:1711824/90‑1 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  2:2251672/90‑1 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  1:718563/90‑1 (MQ=255)                                        ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  1:2783537/90‑1 (MQ=255)                                         tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                          <  2:2723024/90‑1 (MQ=255)                                            gTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGTCCGGTg                                       >  2:73331/1‑90 (MQ=255)                                            gTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTg                                       >  2:1554714/1‑90 (MQ=255)                                            gTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTg                                       >  1:1920079/1‑90 (MQ=255)                                             tACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGa                                      >  2:1913118/1‑90 (MQ=255)                                             tACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGa                                      >  2:2886688/1‑90 (MQ=255)                                             tACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGa                                      >  1:848588/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAcgc                                                                    >  1:598564/1‑56 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAcgc                                                                    <  2:598564/56‑1 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:2725319/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:171818/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:867042/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:422398/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  1:1238795/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:1318021/1‑90 (MQ=255)                                                 ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  1:652201/1‑90 (MQ=255)                                                  cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                 <  2:468488/90‑1 (MQ=255)                                                    agaAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAt                               >  1:793698/1‑90 (MQ=255)                                                    agaAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAt                               >  1:579299/1‑90 (MQ=255)                                                      aaaGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc                             >  1:3003691/1‑90 (MQ=255)                                                      aaaGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc                             >  1:1987575/1‑90 (MQ=255)                                                           cGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCggg                        >  2:2689077/1‑90 (MQ=255)                                                            gATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGc                       >  1:2915206/1‑90 (MQ=255)                                                             aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCa                      >  2:1976479/1‑90 (MQ=255)                                                             aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCa                      >  1:2659832/1‑90 (MQ=255)                                                             aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCa                      >  2:1208286/1‑90 (MQ=255)                                                                 ggTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAtttt                  >  2:1770176/1‑90 (MQ=255)                                                                  gTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 <  2:565568/42‑1 (MQ=255)                                                                  gTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  1:565568/1‑42 (MQ=255)                                                                  gTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAttttt                 >  2:710891/1‑90 (MQ=255)                                                                   tGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAtttttt                >  2:2111127/1‑90 (MQ=255)                                                                   tGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAtttttt                >  1:384322/1‑90 (MQ=255)                                                                    gATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAttttttt               >  1:1841180/1‑90 (MQ=255)                                                                     aTTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTa              <  2:848588/90‑1 (MQ=255)                                                                          ccAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCt         <  1:2515171/90‑1 (MQ=255)                                                                          ccAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCt         >  1:1213247/1‑90 (MQ=255)                                                                           cAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTa        >  2:754286/1‑90 (MQ=255)                                                                            aGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTaa       >  1:2354645/1‑90 (MQ=255)                                                                              aCTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAc     >  1:1781541/1‑90 (MQ=255)                                                                              aCTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAc     >  1:1050821/1‑90 (MQ=255)                                                                               cTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAcc    <  2:2553113/90‑1 (MQ=255)                                                                               cTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAcc    <  1:1253425/90‑1 (MQ=255)                                                                               cTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAcc    <  2:1841180/90‑1 (MQ=255)                                                                               cTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAcc    >  1:971042/1‑90 (MQ=255)                                                                                 gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAt  >  2:2180303/1‑90 (MQ=255)                                                                                 gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAt  >  2:1849132/1‑90 (MQ=255)                                                                                 gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAt  >  1:2838456/1‑90 (MQ=255)                                                                                        |                                                                                   CCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAT  >  NZ_CP009273/130611‑130780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑