Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,503,965	T→C	D67D (GAT→GAC)	hicB →	type II toxin‑antitoxin system antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,503,965	0	T	C	100.0%	211.6 / NA	56	D67D (GAT→GAC)	hicB	type II toxin‑antitoxin system antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

GGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG  >  NZ_CP009273/1503878‑1504049                                                                                        |                                                                                     ggCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCa                                                                                    <  1:2225635/90‑1 (MQ=255)          tGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGa                                                                           <  1:1571173/90‑1 (MQ=255)           gCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGaa                                                                          >  1:2622381/1‑90 (MQ=255)            cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg                                                                         <  2:1410059/90‑1 (MQ=255)            cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg                                                                         <  1:91179/90‑1 (MQ=255)            cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg                                                                         <  2:1746919/90‑1 (MQ=255)              ttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTa                                                                       <  2:2806182/90‑1 (MQ=255)                   gACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCttt                                                                  >  2:1997639/1‑90 (MQ=255)                   gACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCttt                                                                  >  1:2125265/1‑90 (MQ=255)                   gACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCttt                                                                  >  2:908024/1‑90 (MQ=255)                     ccGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGa                                                                >  1:1576619/1‑90 (MQ=255)                     ccGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGa                                                                >  1:29206/1‑90 (MQ=255)                     ccGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGa                                                                >  1:1847825/1‑90 (MQ=255)                        cATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCg                                                             >  1:1755520/1‑90 (MQ=255)                          tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  2:1708014/90‑1 (MQ=255)                          tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  1:377916/90‑1 (MQ=255)                          tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  1:2894698/90‑1 (MQ=255)                          tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                           <  1:1323851/90‑1 (MQ=255)                           ttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          <  1:126746/90‑1 (MQ=255)                             gATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                        <  1:2215945/90‑1 (MQ=255)                             gATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                        <  2:884992/90‑1 (MQ=255)                             gATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                        <  1:59070/90‑1 (MQ=255)                                  ttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          >  2:1414477/1‑83 (MQ=255)                                  ttATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          <  1:1414477/83‑1 (MQ=255)                                          aaGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGc                                           <  2:1311475/90‑1 (MQ=255)                                                   aGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATg                                  >  2:248443/1‑90 (MQ=255)                                                    gCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGc                                 <  1:908024/90‑1 (MQ=255)                                                       tATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCttt                              >  1:1820316/1‑90 (MQ=255)                                                            cTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc                         <  2:1685362/90‑1 (MQ=255)                                                            cTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc                         <  1:560551/90‑1 (MQ=255)                                                            cTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc                         <  2:29206/90‑1 (MQ=255)                                                             tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                        <  2:2469998/58‑1 (MQ=255)                                                             tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                        >  1:2469998/1‑58 (MQ=255)                                                             tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACa                        >  1:1258382/1‑90 (MQ=255)                                                             tttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACa                        >  1:287295/1‑90 (MQ=255)                                                              ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          <  1:1856999/55‑1 (MQ=255)                                                              ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                          >  2:1856999/1‑55 (MQ=255)                                                               tACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGt                      <  1:172474/90‑1 (MQ=255)                                                                   ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGa                  >  1:2180214/1‑90 (MQ=255)                                                                   ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGa                  >  1:2640280/1‑90 (MQ=255)                                                                   ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGa                  >  2:2357990/1‑90 (MQ=255)                                                                   ttCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGa                  >  1:695352/1‑90 (MQ=255)                                                                    tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 >  1:3012243/1‑90 (MQ=255)                                                                    tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 >  1:2107251/1‑90 (MQ=255)                                                                    tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 >  2:316903/1‑90 (MQ=255)                                                                    tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 >  2:889802/1‑90 (MQ=255)                                                                    tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGaa                 >  1:1802105/1‑90 (MQ=255)                                                                         aTTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCAc            <  1:1254500/90‑1 (MQ=255)                                                                         aTTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCAc            <  2:526800/90‑1 (MQ=255)                                                                          ttAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACt           >  1:372233/1‑90 (MQ=255)                                                                          ttAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACt           <  2:572051/90‑1 (MQ=255)                                                                               tAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCa      <  2:287295/90‑1 (MQ=255)                                                                               tAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCa      <  1:2360447/90‑1 (MQ=255)                                                                                 gTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGAATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAag    >  1:1353101/1‑90 (MQ=255)                                                                                   cACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAagag  >  2:2714931/1‑90 (MQ=255)                                                                                   cACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAagag  >  2:2955920/1‑90 (MQ=255)                                                                                        |                                                                                     GGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAG  >  NZ_CP009273/1503878‑1504049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑