| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,505,430 | A→G | K391E (AAA→GAA) | ydcR → | PLP‑dependent aminotransferase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,505,430 | 0 | A | G | 97.1% | 234.6 / NA | 69 | K391E (AAA→GAA) | ydcR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (34/33); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505343‑1505518 | gCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGgaa > 2:2640403/1‑90 (MQ=255)gCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGgaa > 1:2212925/1‑90 (MQ=255) ccATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAAt < 1:350622/90‑1 (MQ=255) cATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAAtt < 1:945820/90‑1 (MQ=255) tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca < 2:22990/90‑1 (MQ=255) tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca < 2:786456/90‑1 (MQ=255) tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca < 2:887385/90‑1 (MQ=255) tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca < 2:895265/90‑1 (MQ=255) ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc < 2:2685325/90‑1 (MQ=255) gtcgCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATa < 1:1462152/90‑1 (MQ=255) ccTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTTGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGa < 2:444377/90‑1 (MQ=255) ccTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGa < 2:1627272/90‑1 (MQ=255) cTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGAc < 2:1500676/90‑1 (MQ=255) gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg < 2:2538139/90‑1 (MQ=255) gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg > 1:2250012/1‑90 (MQ=255) cGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGt < 1:2026829/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc > 2:1044165/1‑90 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 2:2250012/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 2:1967790/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 2:41028/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 1:262333/90‑1 (MQ=255) aCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGtt < 1:2934841/90‑1 (MQ=255) aaaCAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAg < 1:2826306/90‑1 (MQ=255) aacaacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGc < 2:906612/90‑1 (MQ=255) acaacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCt < 2:1745613/90‑1 (MQ=255) aacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcc > 1:2862322/1‑90 (MQ=255) aacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcc > 2:290513/1‑90 (MQ=255) cGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcccc > 2:2070367/1‑90 (MQ=255) cGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcccc > 2:991456/1‑90 (MQ=255) gTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCg > 2:1523020/1‑90 (MQ=255) gTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCg > 2:1074426/1‑90 (MQ=255) tGCCTGGCAGGCCCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGa < 2:608614/90‑1 (MQ=255) tGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGa > 1:1631708/1‑90 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg > 1:1216808/1‑90 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg > 1:862205/1‑90 (MQ=255) ccTGGCAGGCACTGCTGCTTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGc < 2:1918783/90‑1 (MQ=255) ccTGGCAGGCACTGCTGCGTTTTCTGCCTGGGGAAGGGGAAATTCATCATAATGACGGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGc < 1:711534/90‑1 (MQ=255) ccTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGc < 2:1216808/90‑1 (MQ=255) ccTGGCAGGCACTGCTGCGTTATCTGCCGGGGGAAGTGGAAATTCATAAAAATGACAGTGTTTACTTTCTCTGGTTGGAGCTCCCCGAGc < 1:1688873/90‑1 (MQ=255) ggcaggcaCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGt > 2:2338201/1‑90 (MQ=255) ggcaCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGa > 2:2937551/1‑90 (MQ=255) caCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg > 1:1153065/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:2650576/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:2262305/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:2035/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:1546875/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:1268725/1‑90 (MQ=255) tgcGTTATCTGCCTGCGGAAGTGGAAATTCATCAAAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCg < 2:1242401/90‑1 (MQ=255) gTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAAt > 1:1850361/1‑90 (MQ=255) gTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAAt > 1:2386355/1‑90 (MQ=255) gTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAAt > 2:381471/1‑90 (MQ=255) gTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAAt > 2:50271/1‑90 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTTGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 2:1181081/1‑90 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGc < 2:1951393/69‑1 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGc > 1:1951393/1‑69 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 2:1190268/1‑90 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 2:2909054/1‑90 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCGGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTaa < 2:2671877/90‑1 (MQ=255) aTCTGCCTGCGAAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 2:994808/1‑90 (MQ=255) tgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGcc > 1:2368011/1‑90 (MQ=255) gcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCt > 2:592240/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 1:2547205/1‑90 (MQ=255) tgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggc < 1:50271/90‑1 (MQ=255) tgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggc < 2:776138/90‑1 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc > 2:97193/1‑84 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 2:2325886/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCAGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 1:421553/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGc < 1:97193/84‑1 (MQ=255) ggAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcggc < 2:2726363/90‑1 (MQ=255) gAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc < 1:893224/90‑1 (MQ=255) gAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc < 2:1246446/90‑1 (MQ=255) | GCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505343‑1505518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |