Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,573,157	A→T	I148N (ATC→AAC)	yddA ←	ABC transporter ATP‑binding protein/permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,573,157	0	A	T	100.0%	323.0 / NA	81	I148N (ATC→AAC)	yddA	ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (38/43);  total (38/43)

CGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAG  >  NZ_CP009273/1573072‑1573234                                                                                      |                                                                              cgcgCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAg                                                                           >  2:230870/1‑90 (MQ=255)   cgcTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTa                                                                         <  1:2932206/90‑1 (MQ=255)      tttCCCAAAGAATAACGGGAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAaga                                                                      >  1:1863264/1‑90 (MQ=255)       ttCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAagag                                                                     >  2:2066508/1‑90 (MQ=255)         cccAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGaa                                                                   <  1:2111226/90‑1 (MQ=255)             aaGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTc                                                               >  2:1445328/1‑90 (MQ=255)                aaTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTc                                                            <  2:2542948/90‑1 (MQ=255)                aaTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTc                                                            <  1:1808851/90‑1 (MQ=255)                 aTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCa                                                           >  1:1033051/1‑90 (MQ=255)                 aTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCa                                                           >  2:928865/1‑90 (MQ=255)                   aaCGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGc                                                         <  1:2573704/90‑1 (MQ=255)                     cGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGa                                                       <  1:630161/90‑1 (MQ=255)                     cGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGa                                                       <  2:1029244/90‑1 (MQ=255)                         aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                   >  1:392831/1‑90 (MQ=255)                         aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                   >  1:6513/1‑90 (MQ=255)                         aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                   >  1:995378/1‑90 (MQ=255)                         aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                   >  1:1412195/1‑90 (MQ=255)                          aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                  <  2:361875/90‑1 (MQ=255)                          aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                  <  1:279535/90‑1 (MQ=255)                          aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                  <  1:2731660/90‑1 (MQ=255)                             gTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTgg                                               >  2:654268/1‑90 (MQ=255)                              tGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGt                                              <  2:1227463/90‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGt                                                                          >  2:401378/1‑61 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGt                                                                          <  1:401378/61‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATg                                                                 <  2:1505256/70‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATg                                                                 >  1:1505256/1‑70 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             <  1:443774/90‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             >  1:972050/1‑90 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             <  2:1033051/90‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             <  2:2327947/90‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             >  1:1763474/1‑90 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             <  1:1846790/90‑1 (MQ=255)                               gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                             >  2:1846790/1‑90 (MQ=255)                                aTCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa                                            <  2:6513/90‑1 (MQ=255)                                   aGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAggg                                         <  1:2333808/90‑1 (MQ=255)                                    gCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGt                                        <  2:2566442/90‑1 (MQ=255)                                      ctGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                      >  1:2999408/2‑90 (MQ=255)                                      aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                      >  1:1734139/1‑90 (MQ=255)                                      aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                      >  2:2196603/1‑90 (MQ=255)                                      aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                      <  1:1445328/90‑1 (MQ=255)                                        gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc                                    <  2:1710415/90‑1 (MQ=255)                                        gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc                                    <  2:2206871/90‑1 (MQ=255)                                        gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc                                    <  1:1891625/90‑1 (MQ=255)                                        gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTATTCAGCGATACGTTGGTCAGGGTTATc                                    <  2:2426627/90‑1 (MQ=255)                                           aaGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGt                                 <  1:2869351/90‑1 (MQ=255)                                              tGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTAtt                              >  2:2231768/1‑90 (MQ=255)                                              tGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTAtt                              >  2:359650/1‑90 (MQ=255)                                                 cTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAgaga                                                                    >  1:1472303/1‑49 (MQ=255)                                                 cTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAgaga                                                                    <  2:1472303/49‑1 (MQ=255)                                                  tGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTa                          >  1:646841/1‑90 (MQ=255)                                                   ggATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTAt                         >  1:1322529/1‑90 (MQ=255)                                                     aTGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGc                       >  2:1639695/1‑90 (MQ=255)                                                      tGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCt                      <  2:2194283/90‑1 (MQ=255)                                                       gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTTTATTTTTATGCTc                     <  2:1747635/90‑1 (MQ=255)                                                       gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                     <  2:2999408/90‑1 (MQ=255)                                                       gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                     <  1:1673726/90‑1 (MQ=255)                                                       gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                     <  1:654268/90‑1 (MQ=255)                                                       gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                     <  2:1763474/90‑1 (MQ=255)                                                        aaGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTc                                                            <  1:719239/50‑1 (MQ=255)                                                        aaGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTc                                                            >  2:719239/1‑50 (MQ=255)                                                             aaaTGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTa               >  1:2111443/1‑90 (MQ=255)                                                              aaTGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAg              >  1:1362783/1‑90 (MQ=255)                                                                 gAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATc           <  1:928865/90‑1 (MQ=255)                                                                 gAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATc           <  2:1362783/90‑1 (MQ=255)                                                                  aaaGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCt          >  1:2595157/1‑90 (MQ=255)                                                                   aaGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTg         >  2:342504/1‑90 (MQ=255)                                                                    aGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGc        >  2:749790/1‑90 (MQ=255)                                                                      aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt      <  2:972050/90‑1 (MQ=255)                                                                      aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt      <  2:2224905/90‑1 (MQ=255)                                                                      aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt      <  1:2066508/90‑1 (MQ=255)                                                                      aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt      <  1:1760834/90‑1 (MQ=255)                                                                        tGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa    <  1:647523/90‑1 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGc                       <  2:2814924/70‑1 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGc                       >  1:2814924/1‑70 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa   >  2:2824819/1‑90 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa   >  2:2140953/1‑90 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa   >  1:1061463/1‑90 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa    <  2:451689/89‑1 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa    >  1:451689/1‑89 (MQ=255)                                                                         gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGGTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGaa   >  2:2796680/1‑90 (MQ=255)                                                                          aGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAag  >  1:1366297/1‑90 (MQ=255)                                                                                      |                                                                              CGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAG  >  NZ_CP009273/1573072‑1573234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑