Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,659,576	T→C	F14S (TTC→TCC)	clcB →	voltage‑gated ClC‑type chloride channel ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,659,576	0	T	C	100.0%	215.8 / NA	58	F14S (TTC→TCC)	clcB	voltage‑gated ClC‑type chloride channel ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/23);  total (35/23)

CTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAGT  >  NZ_CP009273/1659489‑1659662                                                                                        |                                                                                       ctTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTccc                                                                                      <  1:2156005/90‑1 (MQ=255)      gCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGt                                                                                 >  1:2095841/1‑90 (MQ=255)       ccGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTc                                                                                <  2:918887/90‑1 (MQ=255)       ccGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTc                                                                                <  2:2323735/90‑1 (MQ=255)          cAAAATAGTCGCCCGTGTTTCATTGCCTATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGc                                                                             <  2:2709437/90‑1 (MQ=255)           aaaaTAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCt                                                                            >  2:617110/1‑90 (MQ=255)            aaaTAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCtt                                                                           <  2:77077/90‑1 (MQ=255)                   cGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCaa                                                                    <  2:1836148/90‑1 (MQ=255)                    gCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                   <  2:912132/90‑1 (MQ=255)                    gCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                   <  1:15797/90‑1 (MQ=255)                      ccGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAg                                                                 >  1:2539298/1‑90 (MQ=255)                        gtgtTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtc                                                               <  2:679450/90‑1 (MQ=255)                        gtgtTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtc                                                               >  1:2353312/1‑90 (MQ=255)                               aTTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTa                                                        >  2:287483/1‑90 (MQ=255)                                  gCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATtc                                                     >  1:2655918/1‑90 (MQ=255)                                     cATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTcg                                                  <  1:1017799/90‑1 (MQ=255)                                            gCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCtt                                           >  2:607205/1‑90 (MQ=255)                                            gCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCtt                                           >  2:1666741/1‑90 (MQ=255)                                            gCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCtt                                           >  2:1533420/1‑90 (MQ=255)                                            gCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCtt                                           >  1:135965/1‑90 (MQ=255)                                                 tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtcg                                                              >  2:171765/1‑66 (MQ=255)                                                 tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtcg                                                              <  1:171765/66‑1 (MQ=255)                                                 tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccg                                      >  2:912903/1‑90 (MQ=255)                                                  gCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgt                                     <  1:1653412/90‑1 (MQ=255)                                                      catcTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgttgcc                                 >  1:2636499/1‑90 (MQ=255)                                                         cTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCggg                              >  2:872012/1‑90 (MQ=255)                                                            cacaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGttt                           <  1:234235/90‑1 (MQ=255)                                                            cacaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGttt                           <  2:1086562/90‑1 (MQ=255)                                                             acaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGGATTCTCGCGTCCTTTGCCGTTGCCGGGTTTc                          >  2:2807282/1‑90 (MQ=255)                                                              caTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCg                         >  1:1505830/1‑90 (MQ=255)                                                              caTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCg                         >  2:674001/1‑90 (MQ=255)                                                              caTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCg                         >  1:1431030/1‑90 (MQ=255)                                                               aTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                   >  2:478064/1‑47 (MQ=255)                                                               aTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                   <  1:478064/47‑1 (MQ=255)                                                               aTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGt                        >  2:836698/1‑90 (MQ=255)                                                               aTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGt                        >  2:119382/1‑90 (MQ=255)                                                                tCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTc                       >  1:2700649/1‑90 (MQ=255)                                                                tCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTc                       >  1:905314/1‑90 (MQ=255)                                                                tCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTc                       >  1:702679/1‑90 (MQ=255)                                                                 cTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCa                      >  2:2649063/1‑90 (MQ=255)                                                                  tATCCGGATCTGCGCACTATGTCCCACC‑TCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATg                    >  2:2917722/1‑90 (MQ=255)                                                                   aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATg                    >  1:153136/1‑90 (MQ=255)                                                                   aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATg                    >  1:2145283/1‑90 (MQ=255)                                                                        gATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtg               >  1:2745964/1‑90 (MQ=255)                                                                        gATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtg               <  1:2584690/90‑1 (MQ=255)                                                                          tCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtgct             <  2:407822/90‑1 (MQ=255)                                                                          tCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtgct             <  1:2209402/90‑1 (MQ=255)                                                                          tCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtgct             <  1:674001/90‑1 (MQ=255)                                                                             gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCggg                              <  2:1604698/70‑1 (MQ=255)                                                                             gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCggg                              >  1:1604698/1‑70 (MQ=255)                                                                             gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct          >  2:1274260/1‑90 (MQ=255)                                                                             gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct          >  1:2902849/1‑90 (MQ=255)                                                                             gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGCATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct          >  1:2056902/1‑90 (MQ=255)                                                                                cACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACt       <  2:856529/90‑1 (MQ=255)                                                                                cACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACt       <  1:2994148/90‑1 (MQ=255)                                                                                    aTGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAg   <  2:2700649/90‑1 (MQ=255)                                                                                    aTGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAg   >  2:2113151/1‑90 (MQ=255)                                                                                    aTGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAg   >  1:642326/1‑90 (MQ=255)                                                                                     tGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAGt  <  1:887502/90‑1 (MQ=255)                                                                                        |                                                                                       CTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAGT  >  NZ_CP009273/1659489‑1659662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑