| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,770,507 | 0 | G | T | 58.3% | 9.6 / 18.7 | 12 | V222L (GTG→TTG) | ydiF | acyl CoA:acetate/3‑ketoacid CoA transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base T (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGT > NZ_CP009273/1770428‑1770595 | cTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCa < 1:324973/90‑1 (MQ=255) gAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAg < 2:90348/90‑1 (MQ=255) ctcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGGATTTTTTTGGTGCAGGGGGAGAAAAAGGGTAAGAAAACCACGccg > 1:104610/1‑88 (MQ=255) tcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGc < 2:301311/90‑1 (MQ=255) tcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGc < 2:7294/90‑1 (MQ=255) tcGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGGATTTTGGTGGTGGAGGGGCAGAAAAAGGTTAAGAAAAACAcgccgc > 1:70206/1‑90 (MQ=255) gACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGGTGATGCAGGTGGAGGAAATGGGTAAGAAAGCCACGCTGCAt > 2:56235/1‑90 (MQ=255) aTTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGGATTTTGGTGATGGAGGGGGGGAAAAAGGGTAAAAAAACCACGCCGCATCCTaa > 1:301311/1‑90 (MQ=255) cAGGCGGTGCACAATAACGGCGGGATTTTGGTGATGGAGGGGGAGAAAAAGGGTAAAAAAAACACGCCGCCTCCTTAAAATGTCCGTAtt > 1:360483/1‑90 (MQ=255) gCACAATAACGGCGGGATTTTGATGATGCAGGTGCAGAAAAAGGGTAAAAAAACCACCCCGCCTCCTAAATATTTCCGTATTCCggggtt > 1:116429/1‑87 (MQ=255) aaCGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggtg < 2:406615/90‑1 (MQ=255) aCGGCGGTATTTTGATGATGCAGGGGGGGAAAAAGGTTAAAAAAACCACGCTGCATCCTAAAATTTTCCGTATTTCGGGGTATTtggtgg > 1:406615/1‑90 (MQ=255) tgtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtggt < 2:104610/90‑1 (MQ=255) | CTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGT > NZ_CP009273/1770428‑1770595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |