Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,473,8620TC54.5% 2.7 / 10.5 11P394P (CCT→CCC) dsdAD‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base C (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCAC  >  NZ_CP009273/2473781‑2473933
                                                                                 |                                                                       
cATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCCGGCCCCcgcgcgg                                                                 >  2:144555/1‑89 (MQ=255)
         cATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATc                                                        <  1:134932/90‑1 (MQ=255)
            gCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTGTGGGCGGGCCCCCGCGCGCGTGGGGATTAGt                                                     >  1:74457/1‑90 (MQ=255)
            gCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCCCCGCGCGGGTGTGGATCAGt                                                     >  2:94075/1‑90 (MQ=255)
             cTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTa                                                    <  1:144555/90‑1 (MQ=255)
                    ggctggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGGTGGGCGGGCCCCCGCGCGGGGGGGGATCAGTAAGTTaac                                             >  2:399864/1‑88 (MQ=255)
                                aagaagGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACg                                 <  2:250284/90‑1 (MQ=255)
                                        aTTCGTCTTGAACCTTCGGCACTGGCGGGGGTGGGCGGGCCCCAGCGCGGGTGGGGATCAGGAAATTACCAACAAAAGCGCGGGTTTAgc                         >  1:193857/1‑90 (MQ=255)
                                        aTTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCCCCGCGCGGGGGGGTATCCGTAAAATACCAACAAAAGCGCGGGTTCAgc                         >  1:243164/1‑90 (MQ=255)
                                                       tCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGt          <  2:15072/90‑1 (MQ=255)
                                                               ggCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCAc  <  2:245374/90‑1 (MQ=255)
                                                                                 |                                                                       
CATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGTAAGTTACCAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCAC  >  NZ_CP009273/2473781‑2473933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑