| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,087,995 | 1 | . | G | 26.7% | 25.0 / 13.6 | 15 | coding (445/981 nt) | yggR | type IV pilus twitching motility protein PilT |
| Reads supporting (aligned to +/- strand): ref base . (6/5); new base G (2/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCAAACCAATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCC > NZ_CP009273/3087907‑3088079 | tGCAAACCAATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑g > 2:196530/1‑90 (MQ=255) gCAAACCAATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑gg > 2:151789/1‑90 (MQ=255) aTTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGttga > 2:99281/1‑90 (MQ=255) aTTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGttga > 1:95890/1‑90 (MQ=255) gctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGGCATGTTGATTGAGATAg > 1:81172/1‑90 (MQ=255) gctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGGCATGTTGATTGAGATAg > 1:294145/1‑90 (MQ=255) gATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAAc < 2:135674/90‑1 (MQ=255) aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATc > 2:159191/1‑90 (MQ=255) aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATc < 1:151789/90‑1 (MQ=255) ctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTg < 2:95890/90‑1 (MQ=255) tatTCAACAGGATCTTCCAGCGTCAAAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGCAGATTTGCCa > 2:136838/1‑90 (MQ=255) atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCAc > 2:300614/1‑90 (MQ=255) tCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTc < 1:99281/90‑1 (MQ=255) aCAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTcccc > 2:270356/1‑90 (MQ=255) ccAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCg < 1:159191/90‑1 (MQ=255) gcATCGGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGcc < 2:294145/90‑1 (MQ=255) gcATCGGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGcc < 2:81172/90‑1 (MQ=255) | TGCAAACCAATTTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATC‑GGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCC > NZ_CP009273/3087907‑3088079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |