Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,502,0210TA47.8% 18.5 / 15.0 23E384D (GAA→GAT) yhfWphosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (1/11);  new base A (11/0);  total (12/11)
Fisher's exact test for biased strand distribution p-value = 9.61e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAG  >  NZ_CP009273/3501942‑3502101
                                                                               |                                                                                
aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACgg                                                                        <  1:262527/90‑1 (MQ=255)
     cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAGcc                                                                   >  2:28112/1‑90 (MQ=255)
     cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATTACACACGGGAGcc                                                                   >  2:361541/1‑90 (MQ=255)
     cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGGGTGGGAAAAATCTCACCCCGTAggc                                                                   >  2:211521/1‑88 (MQ=255)
       tAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGcccc                                                                 <  2:128771/90‑1 (MQ=255)
        aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGccccc                                                                <  1:252832/90‑1 (MQ=255)
        aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGccccc                                                                <  1:4779/90‑1 (MQ=255)
         gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCca                                                               <  2:186349/90‑1 (MQ=255)
           gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGAGAAAATCACACCCGGTGGCCCCccca                                                             >  2:342604/1‑90 (MQ=255)
            tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACAt                                                            <  1:329028/90‑1 (MQ=255)
            tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACAt                                                            <  1:126524/90‑1 (MQ=255)
             gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAAACACACAACGTAGCCCCcacacc                                                           >  1:75732/1‑88 (MQ=255)
             gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAGCCCCCCCATc                                                           >  2:329028/1‑90 (MQ=255)
             gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGGGGGAAAAAAAAAAAACCGGAGCCCCcaccac                                                           >  1:304628/1‑87 (MQ=255)
             gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGCGCGGGAAAAATTACACACCGGAGCCCCCCCATc                                                           >  2:317662/1‑90 (MQ=255)
                   tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGCGCGGAAAAAATCAAACACGGGAGCCCCCCCATCACaaaa                                                     >  2:237957/1‑89 (MQ=255)
                    gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACAAACCGGAGCCCCCCCAACAGaaaac                                                    >  2:376600/1‑88 (MQ=255)
                     cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGCGCGCGGGAAAAATCACACACCGGAGCCCCCCCAACAAAAAACg                                                   >  2:233956/1‑90 (MQ=255)
                       aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtg                                                 <  1:28112/90‑1 (MQ=255)
                                   gaaagaaaAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCg                                     <  1:308690/90‑1 (MQ=255)
                                      agtaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                  <  2:117517/87‑1 (MQ=255)
                                      agaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                  <  2:241499/90‑1 (MQ=255)
                                                                      gcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAg  >  2:398275/1‑90 (MQ=255)
                                                                               |                                                                                
AAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAG  >  NZ_CP009273/3501942‑3502101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑