| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,531,777 | 0 | T | G | 54.5% | 7.7 / 10.8 | 11 | V345G (GTC→GGC) | yhgF | Tex family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTG > NZ_CP009273/3531703‑3531865 | ggcggcCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCACTGGCaa > 2:910/1‑90 (MQ=255) cTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGcgaccgac < 2:397444/90‑1 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGGTGCCACCGGCAAACTGGGGGCGACCCacac > 2:283486/1‑90 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGCGGGGCCCCCGGCCAAATGGGGGGGGCCCacac > 1:272744/1‑90 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGCGATGCCACTGGCCAACAGGTGGgggccggaac > 1:336458/1‑86 (MQ=255) tGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGc < 1:283486/90‑1 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGGGGGCGGTGCCCCCGGCAAAAAGGGGGGGGCCGACACCCTTTACCCCCCCACCCGGCAGGCCGCcaa > 1:372479/1‑90 (MQ=255) tACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAagc < 2:372479/90‑1 (MQ=255) cTGGGGTAAAAGTGGCGGGGGGCGATGCCACCGGCAAACTGGGGGGGACCGACACCACTTTTCCCCCCACCGGGCAGGCCGCaaaaaaag > 1:227772/1‑86 (MQ=255) gTAAAAGTGGCGGTGGTCGATGCCCCCGGCAAACTGGGGGGGGCCGACACCCTTTTCCCCCCCACCCGGCAGGCCCCCAAAAAAGCGGTg > 2:351799/1‑90 (MQ=255) aaaGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAcc < 2:247799/90‑1 (MQ=255) tggtCGATGCCACTGGCAAACTGGTGGCGACCGACCCCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTgcgc < 1:910/90‑1 (MQ=255) gtCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtg < 1:325562/90‑1 (MQ=255) | GGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTG > NZ_CP009273/3531703‑3531865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |