| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,953,261 | 0 | T | G | 53.3% | 5.7 / 16.0 | 15 | intergenic (‑88/+111) | ppiC/BW25113_RS25790 | peptidylprolyl isomerase PpiC/type II toxin‑antitoxin system PemK/MazF family toxin |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG > NZ_CP009273/3953173‑3953344 | tGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTa < 1:256179/90‑1 (MQ=255) gAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCaa > 1:376049/1‑90 (MQ=255) gAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCaa > 1:62158/1‑90 (MQ=255) gAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCaa > 2:314507/1‑90 (MQ=255) gAGTGAATAATTGTAAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTAACGATATgggca > 1:263386/1‑87 (MQ=255) ataatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATTGGAAAAAccc > 1:227518/1‑90 (MQ=255) gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAAACCGGGCTTCGCAGGGCCCTc > 1:95313/1‑90 (MQ=255) tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCAGGTGCCTCAAGGGgagga > 1:386589/1‑88 (MQ=255) gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaa < 2:62158/90‑1 (MQ=255) tAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAACCCGGAAAAACCGGGCTTCGCAGGGCCCTCAAGGGGAGAAAAc > 1:190829/1‑90 (MQ=255) agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAACCCGGGCTTCGCAGGTCCCTCCAGGGGAGAAAACTTAGGGGCTCTAt > 1:123715/1‑90 (MQ=255) aGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa < 1:163810/90‑1 (MQ=255) ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAggg < 2:65282/72‑1 (MQ=255) ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg > 2:276050/1‑90 (MQ=255) ggCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAATTAggg > 1:65282/1‑72 (MQ=255) | TGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG > NZ_CP009273/3953173‑3953344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |