| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,884 | C→T | P21L (CCG→CTG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,884 | 0 | C | T | 100.0% | 42.7 / NA | 15 | P21L (CCG→CTG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/9); total (6/9) | |||||||||||
TCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49810‑49965 | tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACctgcctgc < 1:269203/90‑1 (MQ=255) taaaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc < 1:233606/88‑1 (MQ=255) cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCCTGCTGTGGAACCTGCCTGCCGATCTCGCCt > 1:283509/1‑90 (MQ=255) gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc > 1:268509/1‑90 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 1:91666/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 1:221191/90‑1 (MQ=255) gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta < 2:394367/90‑1 (MQ=255) gcgTAGATCTCGTCATGGGCATGGAAAACGCCATGCTGGGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc < 1:196482/88‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 2:263128/1‑90 (MQ=255) ttATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTTCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg > 1:229842/1‑90 (MQ=255) ctCGGCATGGAAAGCCCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGgc < 2:229842/89‑1 (MQ=255) cATGGAAAACGCCATCCTGTGGACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa < 2:7174/90‑1 (MQ=255) aTGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt > 1:37353/1‑61 (MQ=255) aTGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 2:37353/61‑1 (MQ=255) cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa > 1:275179/1‑90 (MQ=255) | TCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGA > NZ_CP009273/49810‑49965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |