Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,623,127	(T)5→4	intergenic (+287/‑24)	yfgG → / → yfgH	YfgG family protein/glycine zipper 2TM domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,623,123	0	T	.	100.0%	15.3 / NA	5	intergenic (+283/‑28)	yfgG/yfgH	YfgG family protein/glycine zipper 2TM domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (3/2);  total (3/2)

CCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCAT  >  NZ_CP009273/2623039‑2623199                                                                                     |                                                                             cccTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTAttttcg                                                                         <  1:6940/90‑3 (MQ=255)    tGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTA‑TTTTCGTaa                                                                     <  1:5156/90‑1 (MQ=255)                                                       tttCCTCTTTTGATGATCTTAAATGTCTTA‑TTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCtgtg                  >  1:326079/1‑90 (MQ=255)                                                          ccTCTTTTGATGATCTTAAATGTCTTA‑TTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCa               >  1:186009/1‑90 (MQ=255)                                                                    tgatCTTAAATGTCTTA‑TTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGt     <  1:38708/90‑1 (MQ=255)                                                                       tCTTAAATGTCTTA‑TTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCAt  >  1:110096/1‑90 (MQ=255)                                                                                     |                                                                             CCCTGCGTGAATTTTAATAAATTTAATCTATCCCTTTATACGCAATACATTTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCAT  >  NZ_CP009273/2623039‑2623199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑