Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,836,838	(C)5→4	coding (312/1335 nt)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,836,834	0	C	.	100.0%	33.1 / NA	9	coding (316/1335 nt)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (5/4);  total (5/4)

GGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATC  >  NZ_CP009273/3836750‑3836916                                                                                     |                                                                                   ggATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGccccac                                                                               >  2:365105/1‑88 (MQ=255)             gCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGc                                                                  >  1:321156/1‑90 (MQ=255)             gCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGc                                                                  >  2:199573/1‑90 (MQ=255)                   gCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGCAaagaa                                                            <  2:268731/90‑1 (MQ=255)                                aaCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcccc                                               <  1:75799/90‑1 (MQ=255)                                                   gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCa                            <  2:198166/90‑1 (MQ=255)                                                     gcCCATCCCGGTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACg                          >  2:119112/1‑90 (MQ=255)                                                               gTCTGCCAGGAGATGCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAg                <  2:63073/90‑1 (MQ=255)                                                                             gCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATc  >  1:310731/1‑90 (MQ=255)                                                                             gCCCATCG‑CCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATc  >  2:116293/1‑90 (MQ=255)                                                                                     |                                                                                   GGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATC  >  NZ_CP009273/3836750‑3836916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑