| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 578,971 | (A)6→5 | intergenic (+380/‑166) | BW25113_RS26085 → / → appY | tail fiber assembly protein/DNA‑binding transcriptional activator AppY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 578,966 | 0 | A | . | 87.5% | 18.2 / ‑0.5 | 8 | intergenic (+375/‑171) | BW25113_RS26085/appY | tail fiber assembly protein/DNA‑binding transcriptional activator AppY |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (4/3); total (4/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GCAAGAGGTTTCAGGTGCGTTGTAGTGAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAATAAAAAACAACATACATATAATAATTTAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATAT > NZ_CP009273/578877‑579047 | gCAAGAGGTTTCAGGTGCGTTGTAGTGAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAATa > 1:332677/1‑90 (MQ=255) agGTTTCAGGTGCGTTGTAGTGAGTTTATGTTAATGAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAATaaaaaa > 1:172894/1‑90 (MQ=255) aGGTGCGTTGTAGTGAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAACAacatac < 1:216234/90‑1 (MQ=255) gTTGTAGTGAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAACAACATACATataa > 1:99570/1‑90 (MQ=255) gAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAacaaca < 1:221681/73‑1 (MQ=255) gAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAacaaca > 2:221681/1‑73 (MQ=255) gAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAACAACATACATATAATAATTTaa > 1:71742/1‑90 (MQ=255) cGTTGAAAAATGTAACTTTGAAATAAGTTAGAAT‑AAAAACAACATACATATAATAATTTAATCTTAAATGAAATTTATTAAAATTTGCaa < 2:71742/90‑1 (MQ=255) gTTGAAAAATGTAACTTTGAAATAAGTTAGAATAAAAAACAACATACATATAATAATTTAATCTTAAATGAAATTTATTAAAATTTGCaa < 2:172894/90‑1 (MQ=255) aGTTAGAAT‑AAAAACAACATACATATAATAATTTAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAatat > 2:171416/1‑90 (MQ=255) | GCAAGAGGTTTCAGGTGCGTTGTAGTGAGTTTATGTTAATAAAAAGCATAGTAAGCGTTGAAAAATGTAACTTTGAAATAAGTTAGAATAAAAAACAACATACATATAATAATTTAATCTTAAATGAAATTTATTAAAATTTGCAAACTATAATTTTGTGTATAAAAATAT > NZ_CP009273/578877‑579047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |